Abstract
The mode of action of the endopolygalacturonase from Fusarium moniliforme was studied towards a series of pectins with different amounts and distribution patterns of substituents. In homogalacturonan, the enzyme hydrolysed the linkages between two galacturonic acid residues according to a multi-chain attack mechanism, and the final products were monomer and dimer. The enzyme was able to degrade methylated pectins with a degree of methylation up to 70 and acetylated homogalacturonans with a degree of acetylation up to 38 to a lower extend but not xylogalacturonans.
When methylated pectins or acetylated homogalacturonan were used as substrates, the final percentage of hydrolysis decreased with increasing the degree of substitution. A blockwise distribution of the methyl-groups was more favourable to a higher velocity than a random one. In every case, the enzyme was shown to follow a multi-chain attack. The final products included methyl esterified oligogalacturonates. The enzyme seemed to degrade first the non-methylated area of the blockwise methylated substrate. The detailed structure of the oligomers was analysed by mass spectrometry, and showed that the enzyme was able to accomodate methylated or acetylated galacturonic acid residues in its active site.
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Bonnin, E., Le Goff, A., Thibault, JF. (2003). Degradation of Pectins with Different Nature, Amount and Distribution of the Substituents by Endopolygalacturonase of Fusarium Moniliforme . In: Voragen, F., Schols, H., Visser, R. (eds) Advances in Pectin and Pectinase Research. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0331-4_28
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DOI: https://doi.org/10.1007/978-94-017-0331-4_28
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