Abstract
The method for total phenol is useful in order to know the efficiency of extraction of phenolics in solvents. This method can be coupled with the use of insoluble matrix, polyvinyl polypyrrolidone (PVPP; binds tannin-phenolics) for measurement of tannins (see below). The results can be expressed as tannic acid equivalent. The nature of tannic acid varies from one commercial source to the other [2]. Tannic acid from Merck was found to be most appropriate; highest efficiency of binding to bovine serum albumin (BSA), the least threshold at which it starts precipitating BSA and also the highest change in absorption per unit change in tannic acid level. Furthermore, there does not seem to be batch-to-batch variation in tannic acid from Merck.
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© 2003 Springer Science+Business Media Dordrecht
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Makkar, H.P.S. (2003). Measurement of Total Phenolics and Tannins Using Folin-Ciocalteu Method. In: Quantification of Tannins in Tree and Shrub Foliage. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0273-7_3
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DOI: https://doi.org/10.1007/978-94-017-0273-7_3
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-6428-8
Online ISBN: 978-94-017-0273-7
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