Abstract
Cutaneous leishmaniasis is present in two different regions of the State of Parana, (South of Brazil): in the Northern area and the Ribeira River Valley. In 1994 there was an outbreak with a total of 1395 leishmaniosis cases. Here, we report on the isolation and characterization of 52 Leishmania stocks isolated between 1995 and 2000 from humans, dogs, and wild reservoir and phlebotomine hosts. The material, obtained by biopsy (punch no. 4) or by aspiration puncture, was inoculated in two media (NNN and Tobbie & Evans) and maintained at a temperature of 24°C. After isolation, parasites were grown in C.C.S. medium (in Erlenmeyer and bottle of Roux) for protein extract preparation, awaiting identification. Isoenzymatic characterisation was made by electrophoresis in starch thick gel, using 13 enzymatic systems. Leishmania stocks were obtained from active lesions of humans coming from 26 counties in the northern region of Parana and 6 from the Adrianópolis borough (Ribeira River Valley). In the northern area of the state stocks were isolated from 8 dogs (Canis familiaris); 1 stock from Nectomys sp., and 3 stocks were isolated from Lutzomyia whitmani. All stocks were identified as Leishmania (Viannia) braziliensis. Fifteen zymodemes were characterized. Zymodemes LMP1 and LPM2 showed a large distribution in both regions studied. These zymodemes are present from the south of Mexico to the North of Argentina.
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Thomaz-Soccol, V. et al. (2003). Leishmania Species in Two Regions of Paraná, Brazil: Biochemical Characterization by Isoenzyme Electrophoresis. In: Roussos, S., Soccol, C.R., Pandey, A., Augur, C. (eds) New Horizons in Biotechnology. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0203-4_35
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