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In Vitro Regeneration and Genetic Transformation of Chickpea

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Applied Genetics of Leguminosae Biotechnology

Part of the book series: Focus on Biotechnology ((FOBI,volume 10B))

Abstract

Chickpea is one of the major grain legume crops of the world and of the Indian subcontinent. Production of chickpea has not made any significant increase in the last few decades as vulnerability of the crop to various diseases and pests seriously hamper the crop yield. Attempts to introgress desirable traits from wild relatives into cultivated chickpea have had limited success due to sexual incompatibilities and high degree of autogamy. Genetic engineering techniques to incorporate useful traits have not made any significant progress for want of an efficient regeneration protocol. Although chickpea has been successfully regenerated in vitro using diverse explants on different media and somatic embryogenesis has been reported in various genotypes of chickpea, rooting of shoots and conversion of embryos to plants is still problematic besides the genotype dependency of the reported methods. Regeneration of plants from protoplast and anther has not been given due attention. Even though the genetic transformation of chickpea has been reported where the primary transformants were confirmed by molecular analysis, the methods were genotype dependent and produced low frequencies of transformed tissue or plantlets. This review presents the current status of research encompassing various aspects of shoot regeneration in tissue culture and genetic transformation of chickpea. Critical analysis and directions for future strategy of research on genetic manipulation of chickpea are also presented.

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Authors and Affiliations

  1. Department of Biosciences, M.D. University, Rohtak, 124 001, India

    Sonia, Rana P. Singh & Pawan K. Jaiwal

  2. Genetic Resources and Enhancement Program, Genetic Transformation Laboratory, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Andhra Pradesh, 502 324, India

    K. K. Sharma

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  1. Sonia
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  2. Rana P. Singh
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  3. K. K. Sharma
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  4. Pawan K. Jaiwal
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  1. Department of Biosciences, M.D. University, Rohtak, 124001, India

    Pawan K. Jaiwal  & Rana P. Singh  & 

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Sonia, Singh, R.P., Sharma, K.K., Jaiwal, P.K. (2003). In Vitro Regeneration and Genetic Transformation of Chickpea. In: Jaiwal, P.K., Singh, R.P. (eds) Applied Genetics of Leguminosae Biotechnology. Focus on Biotechnology, vol 10B. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0139-6_5

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  • DOI: https://doi.org/10.1007/978-94-017-0139-6_5

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