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Microprojectile-Mediated Transformation of Peanut

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Applied Genetics of Leguminosae Biotechnology

Part of the book series: Focus on Biotechnology ((FOBI,volume 10B))

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Abstract

Like many legumes peanut has been recalcitrant to genetic transformation. Here I describe some of the parameters that must be considered when establishing a transformation system with reference to legumes in general and peanut in particular. Firstly, I discuss the relative advantages and disadvantages of organogenic and embryogenie tissue culture systems. A procedure for the efficient conversion of peanut somatic embryos into fertile plants is described. Secondly, consideration is given to the important parameters involved in the optimisation of gene transfer using microprojectiles. Finally, I give a detailed description of a protocol to efficiently transform cultivars in both botanical types of peanut (Spanish and Virginia), by particle bombardment into embryogenie callus derived from mature seeds, followed by single-step selection for hygromycin B resistance. This method produces three to six independent non-chimeric transgenic plants per bombardment of 10 cm2 embryogenie callus. Copy number of integrated transgenes ranged from one to twenty with a mean of four and 57% coexpression of hph and luc or uidA genes coprecipitated on separate plasmids. Potted transgenic plant lines can be regenerated within 9 months of callus initiation or 7 months after bombardment.

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  1. CSIRO Tropical Agriculture, 120 Meiers Rd., Indooroopilly, Queensland, 4068, Australia

    D. Malcolm Livingstone

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  1. Department of Biosciences, M.D. University, Rohtak, 124001, India

    Pawan K. Jaiwal  & Rana P. Singh  & 

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Livingstone, D.M. (2003). Microprojectile-Mediated Transformation of Peanut. In: Jaiwal, P.K., Singh, R.P. (eds) Applied Genetics of Leguminosae Biotechnology. Focus on Biotechnology, vol 10B. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0139-6_12

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  • DOI: https://doi.org/10.1007/978-94-017-0139-6_12

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