Abstract
Calli and shoot cultures of H. perforatum were established. The phenolic compounds accumulated in these types of cultures, over a period of four years, were compared by HPLC-DAD with those produced by in vivo plants. Calli and suspended cells did not accumulate detectable amounts of hypericins or phloroglucinols, independently of the several hormonal variants tested. The major phenolics accumulated by these type of biomass were xanthones and some luteolin type flavones. Besides these types of compounds, shoot cultures also accumulated hypericins, hyperforins, flavonols and caffeioylquinic acids. In shoot cultures the production of hypericins decreased continuously during the 4-year culture period whereas hyperforins accumulation remained similar.
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Dias, A.C.P., Ferreira, M.F. (2002). Production of Phenolics by in Vitro Cultures of Hypericum perforatum: A Case Study. In: Rauter, A.P., Palma, F.B., Justino, J., Araújo, M.E., dos Santos, S.P. (eds) Natural Products in the New Millennium: Prospects and Industrial Application. Proceedings of the Phytochemical Society of Europe, vol 47. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9876-7_36
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DOI: https://doi.org/10.1007/978-94-015-9876-7_36
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