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Plant Regeneration from Cultured Protoplasts

  • Chapter
Morphogenesis in Plant Tissue Cultures

Abstract

The protoplasts isolated from various plant species, organs or tissues, can regenerate cell wall, divide and form cell colonies and calli, followed by plant regeneration via different pathway of morphogenesis, under proper culture conditions. Since the first report of plant regeneration from mesophyll protoplasts of tobacco (Nagata and Takebe, 1971; Nitsch and Ohyama, 1971), the number of species with successful plant regeneration from protoplasts reached about 100 by mid 1980’s (Binding, 1985; Maheshwari et al.,1986). According to Roest and Gilissen (1989), this number increased to 212 during the next 4–5 years. Unfortunately, these authors missed several Chinese contributions which appeared in Chineses language (see Loo and Xu, 1986; Xia, 1992). According to our recent survey (Xu and Xue, 1995), there are 368 species or subspecies of higher plants, covering 161 genera of 46 families, for which plant regeneration from protoplasts has been achieved. Among them, the Solanaceous members appear to be most responsive followed, in the declining order, by Leguminosae, Gramineae, Compositae, Cruciferae, Umbelliferae and Rosaceae (See Table 1).

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References

  • Akashi R & Adachi T (1992) Plant regeneration from suspension cultured-derived protoplasts of apomictic dallisgrass (Paspalum dilatatum Poir.). Plant Sci. 82: 219–225.

    CAS  Google Scholar 

  • Al-Atabee JS, Mulligan BJ & Power JB (1990) Interspecific somatic hybrids of Rudbeckia hirta and Rlaciniata (Compositae). Plant Cell Rep. 8: 517–520.

    Google Scholar 

  • An LJ, Luo XM, Li XW, Li FX, He MY & Hao S (1991) Plant regeneration from mesophyll protoplasts of Astragalus tenuis. Acta Bot. Sinica 33: 38–42.

    Google Scholar 

  • An LJ, Zhang JM, Li FX, Luo XM, He MY & Hao S (1993) Study on high-frequency plant regeneration from monocell suspension cultures of Astragalus grubovii. Acta BotSinica, 35 (Suppl.): 153–156.

    Google Scholar 

  • Arcioni S, Davey MR, dos Santos AVP & Cocking EC (1982) Simatic embryogenesis in tissues from mesophyll and cell suspension protoplasts of Medicago coerulea and M.glutinose. Z. Pflanzenphysiol. 106: 105–110.

    CAS  Google Scholar 

  • Arcioni S, Mariotti D & Pezzotti M (1985) Hedysarum coronarium L. in vitro conditions for plant regeneration from protoplasts and callus of various explants. J. Plant Physiol. 121: 141–148.

    CAS  Google Scholar 

  • Arcioni S, Mariotti D & Pezzotti M (1985) Plant regeneration of Medicago arborea from protoplast culture and preliminary experiments of somatic hybridization with Medicago sativa. GenetAgraria 39: 307.

    Google Scholar 

  • Arcioni S, Mariotti D & Pezzotti M (1985a) Plant regeneration of Medicago arborea from protoplast culture and preliminary experiments of somatic hybridization with Medicago sativa. Genet. Agraria 39: 307.

    Google Scholar 

  • Ahuja PS, Lu DY, Cocking EC & Davey MR (1983b) An assessment of the cultural capabilities of Trifolium repens L. (white clover) and Onobrychis viciifolia Scop. (sainfoin) mesophyll protoplasts. Plant Cell Rep. 2:269–272.

    Google Scholar 

  • Asano Y & Sugiura K (1990) Plant regeneration from suspension culture-derived protoplasts of Agrostic alba L. (Redtop). Plant Sci. 72: 267–273.

    Google Scholar 

  • Attree SM, Bekkaoui F, Dunstan DI & Fowke LC (1987) Regeneration of somatic embryos from protoplasts isolated from an embryogenic suspension culture of white spruce (Picea glauca). Plant Cell Rep. 6: 480–483.

    Google Scholar 

  • Attree SM, Dunstan DI & Fowke LC (1989) Plantlet regeneration from embryogenic protoplasts of white spruce (Picea glauca). Biotechnology 7:1061–1062.

    Google Scholar 

  • Bapat VA, Gill R & Rao PS (1985) Regeneration of somatic embryos and plantlets from stem callus protoplasts of sandalwood tree (Santalum album L.). Curr. Sci. 54:978–982.

    Google Scholar 

  • Barakat MN & Cocking EC (1983) Plant regeneration from protoplast-derived tissues of Linum usitatissimum L. (flax). Plant Cell Rep. 2:314–317.

    Google Scholar 

  • Bhadra SK, Hammatt N, Power JB & Davey MR (1994) A reproducible procedure for plant regeneration from seedling hypocotyl protoplasts of Vigna sublobata L. Plant Cell Rep. 14:174–179.

    Google Scholar 

  • Binding H & Nehls R (1977) Regeneration of isolated protoplasts to plants in Solanum dubcamara L. Z. Planzenphysiol. 85:279–280.

    Google Scholar 

  • Binding H & Nehls R (1980) Protoplast regeneration to plants in Senncio vulgaris L. Z. Pflanzenphysiol. 99:183–185.

    Google Scholar 

  • Binding H (1974) Regeneration of haploid and diploid plants from protoplastts of Petunia hybrida L. Z. Pflanzenphysiol 74: 327–356.

    Google Scholar 

  • Binding H (1985) Regeneration of plants. In: Fowke LC & Constabel F (eds.) Plant Protoplasts (pp:21–27) CRC Press, Boca Raton.

    Google Scholar 

  • Binding H, Bunning D, Gorschen E, Jorgensen J, Kollmann R, Krumbiegal-schroeren G, Ling HQ, Monzer J, Mordhorst G, Rudnick J, Sauer A, Witt D & Zuba M (1988) Uniparental, fusant, and chimeric plants regenerated from protoplasts after streak plating in agarose gels. Plant Cell Tissue Organ Cult. 12:133–135.

    Google Scholar 

  • Binding H, Jain SM, Finger J, Mordhorst G, Nehls R & Gressel J (1982) Somatic hybridization of an atrazine resistant biotype of Solanum nigrum L. Clonal variation in morphology and in ztrazine sensitivity. Theor. Appl. Genet. 63:273–277.

    Google Scholar 

  • Binding H, Nehis R & Kock R (1980) Nersuche zur protoplastenregeneration dikotyler pflanzen unterschiedlicher systematischer zugehorigkeit. Ber. Dtsch.Bot.Ges. 93:667–671.

    Google Scholar 

  • Binding H, Nehls R, Kock R, Finger J & Mordhorst G (1981) Comparative studies on protoplast regeneration in herbaceous species of the dicotyledoneae. Z .Pflanzenphysiol 101:119–130.

    Google Scholar 

  • Binding H, Rgensen J, Krumbiegel-Schroeran G, Finger J, Mordhorst G & Suchowiat G (1983) Culture of apical protoplasts from shoot cultures in the orders Fabales, Tosales, and Caryophyllales. In: Potrykus I et al. (eds.) Protoplasts 1983, Poster Proceedings (pp.90) Birkhauser, Basel.

    Google Scholar 

  • Bokelmann GS & Roest S (1983) Plant regeneration from protoplasts of potato (Solanum tuberosum cv. Bintje). Z.Pflanzenphysiol. 109:259–263.

    CAS  Google Scholar 

  • Burrus M, Chanace C, Alibert G & Bidney D (1991) Regeneration of fertile plants from protoplasts of sunflower (Helianthus annuus L.). Plant Cell Rep. 10:161–166

    CAS  Google Scholar 

  • Caboche M (1980) Nutritional requirements of protoplast-derived, haploid tobacco cells grown at low cell densities in liquid medium. Planta 149:7–18.

    CAS  Google Scholar 

  • Cai QG, Guo ZC, Qian YQ, Jiang RX & Zhao YL (1987) Plant regeneration from protoplasts of corn (Zea mays L.). Acta Bot.Sinica 29:453–459.

    Google Scholar 

  • Carlberg I, Glimelius K & Eriksson T (1984) Nuclear DNA content during the initiation of callus formation from isolated protoplasts of Solanum tuberos um. Plant Sci Lett 35:225–230.

    CAS  Google Scholar 

  • Chand PK, Ochatt SJ, Rech EL, Power JB & Davey MR (1988) Electroporation stimulates plant regeneration from protoplasts of the woody medicinal species Solanum dulcamara L.. J.Exp.Bot. 39:1267–1274.

    Google Scholar 

  • Chen MH & Chen CC (1992) Somatic embryogenesis and plant regeneration from embryo and protoplast culture of Carica. Abstract Symp. on Botany, Inst. of Botany, Acad.Sinica, Taipei, pp:69–70.

    Google Scholar 

  • Chen SC, Tam YY, Chang RL, Chen HR, Len TH & Chang WC (1990) In vitro flowering of ginseng protoplast-derived plantlets. Abstract plant Biotechnology Symp. (Taipei, Nov.19–29, 1990). p.33.

    Google Scholar 

  • Chen WH, Davey MR, Power JB & Cocking EC (1988) Sugarcane protoplasts: factors affecting division and plant regeneration. Plant Cell Rep. 7:344–347.

    CAS  Google Scholar 

  • Chen ZX, Li SJ, Yue IX, Jiao GL, Liu SX, She JM, Wu JY & Wang HB (1989) Plantlet regeneration from protoplasts isolated from an embryogenic suspension culture of cotton (Gossypium hirsutum L.) Acta Bot. Sinica 31:966–969.

    Google Scholar 

  • Coulibaly MY & Demarly Y (1986) Regeneration of plantlets from protoplasts of rice, Oryza sativa L.. Z.Pflanzenzucht. 96:79–81.

    Google Scholar 

  • Creissen GP & Karp A (1985) Karyotypic changes in potato plants regenerated from protoplasts. Plant Cell Tissue Organ Cult. 4:171–182.

    Google Scholar 

  • Dalton SJ (1988a) Plant regeneration from cell suspension protoplasts of Festuca arundinacea Schreb.(ta11 fescue) and Lolium perenne L.(perennial ryegrass). J.Plant Physiol. 132:170–175.

    Google Scholar 

  • Dalton SJ (1988b) Plant regeneration from cell suspension protoplasts of Festuca arundinacea Schreb. Lolium perenne L. and L.multiflorum Lam.. Plant Cell Tissue Organ Cult. 12:137–140.

    Google Scholar 

  • Davey MR & Power JB (1980) Aspects of protoplast culture and plant regeneration. Plant Cell Tissue Organ Cult. 112:115–125.

    Google Scholar 

  • Davey MR, Bush E & Power JB (1974) Cultural studies of a dividing legume leaf protoplast system. Plant Sci.Lett. 3:127–133.

    Google Scholar 

  • Debeaujon I & Branchard M (1992) Induction of somatic embryogenesis and caulogenesis from cotyledon and leaf protoplast-derived colonies of melon (Cucumis melo L.). Plant Cell Rep. 12:37–40.

    Google Scholar 

  • Deng JJ & Xian JA (1989) Plantlet regeneration Setaric italica protoplasts. Plant Physiol.Commic. (Shanghai) (2):56–57.

    Google Scholar 

  • Deng XX, Deng ZA & Wan SY (1989) Formation of embryogenic calli and regeneration of plants from protoplasts of Citrus sinensis (cv. Jincheng) and Fortunella hindsii. Chinese Bull. Agr. 3:13–15.

    Google Scholar 

  • Deng XX, Zhang WC & Wan SY (1988) Studies of the isolation and plant regeneration of protoplast in Citrus. Acta Hortic. Sinica 15:99–102.

    Google Scholar 

  • Dhir SK, Dhir S & Wildhom JM (1991) Plantlet regeneration from immature cotyledon protoplasts of soybean (Glycine max L.). Plant Cell Rep. 10:39–43.

    Google Scholar 

  • Ding AP, Wang HP & Cao YP (1994) Protoplast culture and plant regeneration of Malus pumila. Acta Bot.Sinica 36:271–277.

    Google Scholar 

  • Dong JJ & Xia ZA (1990) Protoplast culture and plant regeneration of foxtail amillet. Chinese Sci. Bull. 35:1560–1564.

    Google Scholar 

  • Donn G (1978) Cell division and callus regeneration from leaf protoplasts of Vicia narbonensis. Z.Pflanzenphysiol. 86:65–75.

    CAS  Google Scholar 

  • Dos Santos AVP, Davey MR & Cocking EC (1983) Cultural studies of protoplasts and leaf callus of Trigonella corniculata and T.foenum-graecum Z. Pflanzenphysiol. 109:227–234.

    Google Scholar 

  • Dos Santos AVP, Outka DE, Cocking EC and Davey MR (1980) Organogenesis and somatic embryogenesis in tissues derived from leaf protoplasts and leaf explants of Medicago sativa. Z.Planzenphysiol. 99:261–270.

    Google Scholar 

  • Dudits D, Kao KN, Constabel F & Gamborg, OL (1976) Embryogenesis and formation of tetraploid and hexaploid plants from carrot protoplasts. Can. J.Bot. 54:1063–1067.

    CAS  Google Scholar 

  • Eigel L & Koop HU (1989) Nurse culture of individual cells: Regeneration of colonies from single protoplasts of Nicotiana tabacum, Brassica napus and Hordeum vulgare. J.Plant Physiol. 134: 577–581.

    Google Scholar 

  • Engler DE & Grogan RG (1984) Variation in lettuce plants regenerated from protoplasts. J.Hered 75:426–430.

    Google Scholar 

  • Eriksson T & Johansson L. V Intl.Cogr.On Plant Tissue and Cell Culture -Abstracts, IAPTC,p.210.

    Google Scholar 

  • Eriksson TR (1985) Protoplast isolation and culture. In: Fowke LC & Constabel F (eds.) Plant Protoplasts (pp.1–19) CRC Press.Inc.Boca Raton, Florida

    Google Scholar 

  • Ferreira PCG, Hemerly AS, de Almeida Engler J, Van Montagu M, Engler G & Inze D (1994) Developmental expression of the Arabidopsis cyclin gene cycl At. Plant cell 6:1763–1774.

    PubMed  CAS  Google Scholar 

  • Fu YY, Jia SR & Lin Y (1985) Plant regeneration from mesophyll protoplast culture of cabbage (Brassica oleracea var. capitata). Theor.Appl.Genet. 71: 495–499.

    Google Scholar 

  • Fujimura T, Sakurai M, Akagi H, Negishi T & Hirose A (1985) Regeneration of rice plants from protoplasts. Plant Tissue Cult. Lett. 2:74–75.

    Google Scholar 

  • Galbraith DW & Shields BA (1982) The effects of inhibitors of cell wall synthesis on tobacco protoplast development. Physiol.Plant. 55:25–30.

    CAS  Google Scholar 

  • Gamborg OL, Davis BP & Stahlhut RW (1983) Cell division and differentiation on protoplasts from cell cultures of Glycine species and leaf tissues of soybean. Plant Cell Rep. 2:213–215.

    Google Scholar 

  • Ge Kouling, Wang Yunzhu, Yuan Xumnei, Huang Peiming, Yang Jinshui, Nie Zhipin, Testa D & Lee N (1989) Plantlet regeneration from protoplasts isolated from mesophyll cells of adsuke bean (Phoseolus angularis, Wight). Plant Sci. 63:209–216.

    Google Scholar 

  • Gill R & Eapen S (1986) Plant regeneration from hypocotyl protoplasts of mothbean (Vigna aconitifolia). Curr. Sci. 55:100–102.

    Google Scholar 

  • Gill R, Rashid A & Maheshwari SC (1979) Isolation of mesophyll protoplasts of Nicotiana rustica and their regeneration into plants flowering in vitro. Physiol.Plant 47:7–10.

    CAS  Google Scholar 

  • Gilmour DM, Davey MR & Cocking EC (1987) Plant regeneration from cotyledon protoplasts of wild Medicago species. Plant Sci 48:107–112.

    Google Scholar 

  • Gihnour DM, Davey MR, Cocking EC & Pental D (1987) Culture of low numbers of forage legume protoplasts in membrane chambers. J.Plant Physiol. 126:457–465.

    Google Scholar 

  • Glimelius K (1984) High growth rates and regeneration capacity of hypocotyl protoplasts in some Brassicaceae. Physiol.Plant. 61:38–44.

    CAS  Google Scholar 

  • Golds TJ, Babczinsky J & Mordhorst AP (1994) Protoplast preparation without centrifugation: plant regeneration of barley (Hordeun vulgare L.). Plant Cell Rep. 13:188–192.

    CAS  Google Scholar 

  • Gosch G, Bajaj YPS & Reinert J (1975) Isolation, culture, and induction of embryogenesis in protoplasts from cell-suspensions of Atropa belladonna. Protoplasma 86:405–410.

    CAS  Google Scholar 

  • Gresshoff PM (1980) In vitro culture of white clover: callus, suspension, protoplast culture, and plant regeneration. Bot.Gaz. 141:157–164.

    Google Scholar 

  • Grosser JW & Collins GB (1984) Isolation and culture of Trifolium rubens protoplasts with whole plant regeneration. Plant Sci. Lett. 37:165–170.

    Google Scholar 

  • Guo GQ, Xia GM, Li ZY & Chen HM (1990) Direct somatic embryogenesis and plant regeneration from protoplast-derived cells of wheat (Triticum aestivum L.). Sci.Sinica 9:970–974.

    Google Scholar 

  • Gupta JN, Kaur R & Cheema GS (1994) Abstract 8th Intern. Congress of Plant Tissue and Cell Culture, p.30. Firenze, Italy.

    Google Scholar 

  • Hammatt N, Knn HA, Davey MR, Nelson RS & Cocking EC (1987) Plant regeneration from cotyledon protoplasts of Glycine canescens and C.clandestina. Plant Sci. 48:129–135.

    Google Scholar 

  • Harris R, Wright M, Byrne M, Varnum J, Brightwell B & Schubert K (1988) Callus formation and plantlet regeneration from protoplasts derived from suspension cultures of wheat (Triticum aestivem L.). Plant Cell Rep. 7:337–340.

    Google Scholar 

  • Hartmann S, Lang H & Reuther G (1992) Differentiation of somatic embryos from protoplasts isolated from embryogenic suspension cultures of Abies alba L.. Plant Cell Rep. 11:554–557.

    Google Scholar 

  • He Z, Cai Q, Ke S, Qian Y & Xu L (1995) Cytogenetic sutdies on regenerated plants derived from protoplasts of Actinidia deliciosa. I. Variation of chromosome number of somatic cells. J.Wuhan Bot.Res. 13:97–101.

    Google Scholar 

  • Heyser JW (1984) Callus and shoot regeneration from protoplasts of proso millet (Panicum miliaceum L.). Z.Pflanzenphysiol. 113:293–299.

    CAS  Google Scholar 

  • Hohn PB, Knudsen S, Mouritzen R, Negri D, Olsen FL & Roue C (1994) Regeneration of fertile barley plants from mechanically isolated protoplasts of the fertilized egg cell. Plant Cell 6:531–543.

    Google Scholar 

  • Holm PB, Knudsen S, Mouritzen R, Negri D, Olsen FL & Roue C (1995) Fertile barley plants can be regenerated from mechanically isolated protoplasts of fertilized egg cells. In: Terzimn M et al.(eds). Current Issue in Plant Molecular and Cellular Biology, (pp. 213–218) Kluwer Academic Publishers, Dordrecht.

    Google Scholar 

  • Horn ME, Conger BV & Harms CT (1988) Plant regeneration from protoplasts of embryogenic suspension cultures of orchardgrass (Dactylis glomerata L.) Plant Cell Rep. 7:371–374.

    Google Scholar 

  • Jahne A, Lazzeri PA & Lorz H (1991) Regeneration of fertile plants from protoplasts derived from embryogenic cell suspensions of barley (Hordeum vulgare L.). Plant Cell Rep. 10:1–6.

    Google Scholar 

  • Jia JF, Wu NF, Shi YJ & Zhang Q (1989) Plant regeneration from callus protoplasts in sainfoin (Onobrychis viciaefolia scop.). Acta Biol. Exp. Sinica, 22:353–355.

    Google Scholar 

  • Jones H, Karp A & Jones MGK (1980) Isolatzon, culture and regeneration of plants from potato protoplasts. Plant Cell Rep. 8:307–310.

    Google Scholar 

  • Kameya T (1975) Culture of protoplasts from chimeral plant tissue of nature. Japan. J. Genet. 50:417–420.

    Google Scholar 

  • Kamo KK, Chang K1, Lynn ME & Hodges TK (1987) Embryogenic callus formation from maize protoplasts. Planta 172:245–251.

    CAS  Google Scholar 

  • Kao KN & Michayluk MR (1975) Nutrient requirements for growth of Vicia hajastana cells and protoplasts at very low population density in liquid media. Planta 126:105–110.

    CAS  Google Scholar 

  • Kao KN & Michayluls MR (1980) Plant regeneration from mesophyll protoplasts of alfalfa. Z.Pflanzenphysiol. 96:135–141.

    Google Scholar 

  • Kirti PB (1988) Somatic embryogenesis in hypocotyl protoplast culture of rapeseed (Brassica napus L.). Plant Breeding 100:222–224.

    Google Scholar 

  • Klein AS, Montezinos D & Delmer DP (1981) Cellulose and 1,3-glucan synthesis during the early stages of wall regeneration in soybean protoplasts. Planta 152:105–114.

    CAS  Google Scholar 

  • Klimaszewska K (1989) Recovery of somatic embryos and plantlets from protoplast cultures of Larix × eurolepis. Plant Cell Rep. 8:440–444.

    Google Scholar 

  • Kobayashi S, Uchimiya H & Ikeda I (1983) Plant regeneration from ‘Trovita’ orange protoplasts. Japan J.Breed. 33:119–122.

    Google Scholar 

  • Kolenbach HW, Wanzel G & Hoffman F (1982) Regeneration of Brassica napus plantlets in cultures from isolated protoplasts of haploid stem embryos as campared with leaf protoplasts. Z.Pflanzenphysiol. 105:131–142.

    Google Scholar 

  • Kouider M, Hauptamann R, Widholm JM, Skirvin RM & Korban SS (1984) Callus formation from Malus x domestica cv. “Jonathan” protoplasts. Plant Cell Rep. 3:142–145.

    Google Scholar 

  • Kranz E & Lorz H In Vitro fertilization with isolated, single gametes results in zygotic embryogenesis and fertile maize plants. Plant Cell 5:739–746.

    Google Scholar 

  • Krasnyanski S, Polgar Z, Nemeth G & Lupotto E (1992) Plant regeneration from callus and protoplast cultures of Helianthus giganteus L.. Plant Cell Rep. 11:7–10.

    CAS  Google Scholar 

  • Kunitake H & Mii M (1990) Somatic embryogenesis and plant regeneration from protoplasts of asparagus (Aspaaragus officinalis L.). Plant Cell Rep. 8:706–710.

    CAS  Google Scholar 

  • Kyozuka J, Hayashi Y & Shimamoto K (1987) High frequency plant regeneration from rice protoplasts by novel nurse culture methods. Mol. Gen. Genet. 206: 408–413.

    CAS  Google Scholar 

  • Lehminger-Mertens R & Jacobsen HJ (1989) Plant regeneration from pea protoplasts via somatic embryogenesis. Plant Cell Rep. 8:379–382.

    Google Scholar 

  • Li LC & Kohlenbach HW (1982) Somatic embryogeneis in quite a direct way in cultures of mesophyll protoplasts of Brassica napus L.. Plant Cell Rep. 1:209–211.

    Google Scholar 

  • Li LC, Chen YM & Chen Y (1988) Studies on protoplast culture of rice (Oryza sativa L.) and plant regeneration from protoplast-derived calli. Acta Genet. Sinica 15:321–328.

    Google Scholar 

  • Li SJ, Meng ZZ, Sheng FJ & Li DB (1992) Plant regeneration from mesophyll protoplasts from axenic of Chinese cabbage (Brassica pekinensis Rupr.) Chinese J.Biotech. 8:249–254.

    CAS  Google Scholar 

  • Li WA & Wang YQ (1979) Observation on the induction of flower bud initiation from mesophyll cell protoplast culture of Nicotiana rustica. Acta Phytophysiol.Sinica 5:379–384.

    Google Scholar 

  • Li WA (1987) Flower development from Nicotiana rustica mesophyll protoplasts. Sci.Sinica (Ser.B) 30:481–485.

    Google Scholar 

  • Li XB & Xu ZH (1997) Protoplast culture of leguminous plants. In: Xu ZH & Wei ZM (eds.) Plant Protoplast Culture and Genetic Transformation. Chapter 11 (p. 105). Shanghai Sci. & Tech. Publ. Shanghai.

    Google Scholar 

  • Li XB, Xu ZH, Wei ZM & Bai YY (1993) Somatic embryogenesis and plant regeneration from protoplasts of cowpea (Vigna sinensis). Acta Bot.Sinica, 35:632–636.

    Google Scholar 

  • Li XQ, Chatrit P, Mathieu C, Vedel F, Paepe D, Remy R & Ambard-Bretteville F (1980) Regeneration of cytoplasmic male sterile protoclones of Nicotiana sylvestris with motochondrial variations. Curr.Genet. 13:261–266.

    Google Scholar 

  • Li Z, Xia GM & Chen HM (1992) Somatic embryogenesis and plant regeneration from protoplasts isolated from embryogenic cell suspensions of wheat (Triticum aestivem L.). Plant Cell Tissue Organ Cult. 28:79–85.

    Google Scholar 

  • Liao ZZ, Chen MZ, Liao QX, Yan QS & Zhang YQ (1994) Plant regeneration from protoplasts of sugarcane. Acta Bot.Sinica, 36:375–379.

    Google Scholar 

  • Lillo C & Shahin EA (1986) Rapid regeneration of plants from hypocotyl protoplasts and root segments of cabbage. Hort.Sci. 21:315–317.

    CAS  Google Scholar 

  • Ling HQ & Binding H (1987) Plant regeneration from protoplasts in Linum. Plant Breed. 98:312–317

    Google Scholar 

  • Liu CM & Xu ZH (1988) Plant regeneration and transformation of mesophyll protoplasts of Nicotiana glutinosa L.. Acta.Biol.Exp.Sinica 21:273–283.

    Google Scholar 

  • Loo SW & Xu ZH (1986) Recent advances in plant tissue and cell culture work in China. Advances in Science of China, Biology 1:295–354.

    Google Scholar 

  • Lu CY, Vasil V & Vasil IK (1981) Isolation and culture of protoplasts of Panicum maximum Jacq. (guinea grass):somatic embryogenesis and plantlet formation. Z.Planzenphysiol. 104:311–318.

    Google Scholar 

  • Lu DY, Davey MR & Cocking EC (1982) Somatic embryogenesis from mesophyll protoplasts of Trigonella corniculata (Leguminosae). Plant Cell Rep. 1:278–280.

    CAS  Google Scholar 

  • Lu DY, Davey MR & Cocking EC (1983) A comparison of the cultural behaviour of protoplasts from leaves, cotyledons and roots of Medicago sativa. Plant Sci.Lett. 31:87–99.

    Google Scholar 

  • Lu DY, Li FL, Chen YM & Chen Y (1987) Somatic embryogenesis and plant regeneration from protoplasts and explants of seedlings of crownvetch (Forage legume). Acta Biol.Exp.Sinica, 20:31–39.

    Google Scholar 

  • Lu DY, Pental D & Cocking EC (1982) Plant regeneration from seedling cotyledon protoplasts. Z. Pflanzenphysiol. 107: 59–63.

    Google Scholar 

  • Luhrs R & Lorz H (1988) Initiation of morphogenic cell-suspension and protoplast cultures of barley (Hordeum vulgare L.). Planta 175:71–81.

    Google Scholar 

  • Luo XM, Zhao GL, Xie XJ, Liu YZ, He MY & Hao S (1991) Plant regeneration from protoplast culture of Astragalus huangheensis. Acta Genet.Sinica, 18:239–243.

    Google Scholar 

  • M et al. (eds.) Current Issues in Plant Molecular & Cellular Biology (pp:213–218) Kluwer Acad.Pulb., Dordrecht.

    Google Scholar 

  • Maheshowari SC, Gill R, Maheshwari N & Gharyal PK (1986) Isolation and regeneration of protoplasts from higher plants. In: Reinert J & Binding H (eds.) Differentiation of Protoplasts and of Transformed Plant Cells (pp:3–36) Springer, Berlin.

    Google Scholar 

  • Malaure RS, Davey MR & Power JB (1990) Plant regeneration from protoplasts of Felicia and Brachycome. Plant Cell Rep. 9:109–112.

    Google Scholar 

  • Mariotti D, Arcioni S & Pezzotti M (1984) Regeneration of Medicago arborea L. plants from tissue and protoplast cultures of different organ origin. Plant Sci.Lett. 37:149–156.

    Google Scholar 

  • Meijer EGM & Steinbiss HH (1983) Plantlet regeneration from suspension and protoplast cultures of the tropical pasture legume Stylosanthes guyanensis (Aubl.). Sw.Ann.Bot. 52:305–310.

    Google Scholar 

  • Merkle SA & Sommer HE (1987) Regeneration of Liriodendron tulipifera (family Magnoliaceae) from protoplast culture. Am.J.Bot. 74:1317–1321.

    Google Scholar 

  • Meyer Y & Cooke R (1979) Time course of hormonal control of the first mitosis in tobacco mesophyll protoplasts cultivated in vitro. Planta 147:181–185.

    CAS  Google Scholar 

  • Meyer Y & Herth W (1978) Chemical inhibition of cell wall formation and cytokinesis, but not of nuclear division in protoplasts of Nicotiana tabacum L. cultured in vitro. Planta 142:253–262.

    CAS  Google Scholar 

  • Mii M & Ohashi H (1988) Plantlet regeneration from protoplasts of kiwifnuit, Actinidia chinensis. Acta.Hort. 230:167–170.

    Google Scholar 

  • Millam SR, Burns ATH, Hocking TJ & Cattell KJ (1988) Effects of a Ficollagarose system on early division and development of mesophyll protoplasts of winter oilseed rape (Brassica napus L.) Plant Cell Tissue Organ Cult. 12:285–290.

    Google Scholar 

  • Nagata T & Takebe I (1971) Plating of isolated tobacco mesophyll protoplasts on agar medium. Planta 99:12–20.

    Google Scholar 

  • Narasimhulu SB & Chopra VL (1988) Species specific shoot regeneration response of cotyledonary explants of Brassicas. Plant Cell Rep. 7:104–106.

    Google Scholar 

  • Negrutiu I & Mousseau J (1980) Protoplasts culture from in vitro grown plants in Nicotiana sylvestris Spegg. and Comes. Z.Pflanzenphysiol. 100:373–379.

    Google Scholar 

  • Newell CA & Luu HT (1985) Protoplast culture and plant regeneration in Glycine canescens F. J. Herm. Plant Cell Tissue Organ Cult. 4:145–149.

    Google Scholar 

  • Nielsen KA, Larsen E & Knudsen E (1993) Regeneration of protoplast-derived green plants of kentucky blue grass (Poa pratensis L.). Plant Cell Rep. 12:537–540.

    CAS  Google Scholar 

  • Nitsch JP & Ohyama K (1971) Obtention de plantes a partir de protoplasts haploides cultives in vitro. C.R.Acad.Sci.(Paris) 273:801–804.

    CAS  Google Scholar 

  • Ochatt SJ & Caso OH (1986) Shoot regeneration from leaf mesophyll protoplasts of wild pear (Pyrus communis var. Pyraster L.). J.Plant Physiol. 122:243–249.

    CAS  Google Scholar 

  • Ochatt SJ & Power JB (1988) Plant regeneration from mesophyll protoplasts of Williams’ Bon Chretien (Syn.Bartiett) pear (Pyrus communis L.). Plant Cell Rep. 7:587–589.

    Google Scholar 

  • Ochatt SJ (1990) Plant regeneration from root callus protoplasts of sourberry (Prunus cerasus L.). Plant Cell Rep. 9:268–271.

    Google Scholar 

  • Ochatt SJ (1992) The development of protoplast-to-tree systems for Prunus cerasifera and Prunus spinosa. Plant Sci. 81:253–259.

    CAS  Google Scholar 

  • Ochatt SJ, Chand PK, Rech EL, Davey MR & Power JB (1988) Electroporation-mediated improvement of plant regeneration from colt cheny (Prunus avium × Pseudocerasus) protoplasts. Plant Sci. 54:165–169.

    Google Scholar 

  • Ochatt SJ, Cocking EC & Power JB (1987) Isolation, culture and plant regeneration of colt cherry (Prunus avium x pseudocerasus) protoplasts. Plant Sci. 50:139–143.

    Google Scholar 

  • Oka S & Ohyama K (1985) Plant regeneration from leaf mesophyll protoplasts of Broussonetia kazinaki Sieb. (paper mulbeny). J.Plant Physiol. 119:455–460.

    Google Scholar 

  • Oliviea MM & Pais MSS (1991) Plant regeneration from protoplasts of long-term callus cultures of Actinidia deliciosa var. deliciosacv. Hayward (kiwifruit). Plant Cell Rep. 9:643–646.

    Google Scholar 

  • Park YG & Son SH (1992) In vitro shoot regeneration from leaf mesophyll protoplasts of hybrid poplar (Populus nigra × P.maximowiczii). Plant Cell Rep. 11:2–6.

    CAS  Google Scholar 

  • Perales EH & Schieder O (1993) Plant regeneration from leaf protoplasts of apple. Plant Cell Tissue Organ Cult. 34:71–76.

    CAS  Google Scholar 

  • Petersen WL, Sulc S & Armstrong CL (1992) Effect of nurse cultures on the production of macro-calli and fertile plants from maize embryogenic suspension culture protoplasts. Plant Cell Rep. 10:591–594.

    Google Scholar 

  • Pillai V, Davey MR & Power JB (1990) Plant regeneration from mesophyll protoplasts of Centaurea cyanus, Senecio × Hybridus and Callistephus chinensis. Plant Cell Rep. 9:402–405.

    Google Scholar 

  • Power JB & Beny SF (1979) Plant regeneration from protoplasts of Browallia viscosa. Z.Planzenphysiol. 94:469–471.

    Google Scholar 

  • Pua EC (1990) Somatic embryogenesis and plant regeneration form hypocotyl protoplasts of Brassica juncea (L.) Czern & Coss. Plant Sci 68:231–238.

    CAS  Google Scholar 

  • Puonti-Kaerlase J & Eriksson T (1988) Improved protoplast culture and regeneration of shoots in pea (Pisum sativum L.). Plant Cell Rep. 7:242–245.

    Google Scholar 

  • Rao NR, Mehta U & Mohan Ram HY (1982) Whole plant regeneration form cotyledonary 3.protoplasts of Crotalaria juncea. In: Fujiwara A (ed.) Plant Tissue Culture (pp.595–596) Japanese Association for Plant Tissue Culture, Tokyo.

    Google Scholar 

  • Rao PS & Ozias-Akins P (1985) Plant regeneration through somatic embryogenesis in protoplast cultures of sandalwood (Santalum album L.). Protoplasma 124:80–86.

    Google Scholar 

  • Rasheed JH, Al-Mallah MK, Cocking EC & Davey MR (1990) Root hair protoplasts of Lotus corniculatus L. (birdsfoot trefoil) express their totipotency. Plant Cell Rep. 8:65–569.

    Google Scholar 

  • Ren YG, Jia JF & Zheng GC (1990) Plantlet regeneration from protoplasts of millet (Setaria italica L.). Chinese J.Bot. 2:103–108.

    Google Scholar 

  • Rhodes CA, Lowe KS & Ruby KL (1988) Plant regeneration from protoplasts isolated from embryogenic maize cell cultures. Biotechnology 6:56–60.

    Google Scholar 

  • Robertson D & Earle ED (1986) Plant regeneration from leaf protoplasts of Brassica oleracea var. italica cv. Green Comet broccoli. Plant Cell Rep. 5: 61–64.

    Google Scholar 

  • Robertson D, Earle ED & Mutschler MA (1988) Increased totipotency of protoplasts from Brassica oleracea plants previously regenerated in tissue culture. Plant Cell Tissue Organ Cult. 1415–24.

    Google Scholar 

  • Roest S & Gilissen LJW (1989) Plant regeneration from protoplasts: a literature review. Acta Bot.Nearl 38:1–23.

    Google Scholar 

  • Russell JA & McCown BH (1986) Culture and regeneration of Populus leaf protoplasts isolated from non-seedling tissue. Plant Sci. 46:133–142.

    Google Scholar 

  • Russell JA & McCown BH (1988) Recovery of plants from leaf protoplasts of hybrid-poplar and aspen clones. Plant Cell Rep. 7:59–62.

    Google Scholar 

  • Saxena PK & Gill R (1987) Plant regeneration from mesophyll protoplasts of the tree legume Pithecellobium dulce Benth. Plant Sci. 53:257–262.

    CAS  Google Scholar 

  • Schieder O (1980) Somatic hybrids between a herbaceous and two tree-Datura speies. Z. Pflanzenphysiol. 98 :119–127.

    Google Scholar 

  • Schopke C, Muller LE & Kohlenbach HW (1987) Somatic embryogenesis and regeneration of plantlets in protoplast culture from somatic embryos of coffee (Coffea canephora P.ex Fr). Plant Cell Tissue Organ Cult. 8:243–248.

    Google Scholar 

  • Scowcroft WR, Davey MR & Power JB (1973) Crown gall protoplasts-isolation, culture and ultrastructure. Plant Sci. Lett. 1:451–456.

    Google Scholar 

  • Shahin EA & Shepard JF (1980) Cassava mesophyll protoplasts: isolation, proliferation, and shoot formation. Plant Sci. Lett. 17:459–465.

    CAS  Google Scholar 

  • She JM, Zhou HY, Lu WZ, Wu HM, Li XH & Sun YR (1990) Characters of the regenerated plants and their progenies (Rz) from rice protoplasts. Acta Genet.Sinica 17:438–442.

    Google Scholar 

  • Shekhawat NS & Galston AW (1983 a) Mesophyll protoplasts of fenugreek (Trigonella foenum-graecum): isolation, culture and shoot regeneration. Plant Cell Rep. 2:119–121.

    CAS  Google Scholar 

  • Shekhawat NS & Galston AW (1983 b) Isolation, culture and regeneration of moth bean Vigna aconitifolia leaf protoplasts. Plant Sci. Lett. 32:43–51.

    Google Scholar 

  • Shepard JF (1980) Abscisic acid-enhanced shoot initiation in protoplast-derived calli of potato. Plant Sci. Lett. 18:327–333.

    CAS  Google Scholar 

  • Shepard JF (1980) Mutant selection and plant regeneration from potato mesophyll protoplasts. In: Rubenstein I et al. (eds.) Genetic Improvement of Crops. (pp:185–219).

    Google Scholar 

  • Sim GE, Loh CS & Goh CJ (1988) Direct somatic embryogenesis from protoplasts of Citrus mitis Blanco. Plant Cell Rep. 7:418–420.

    Google Scholar 

  • Sinha RR & Das K (1986) Anther-derived callus of Dolichos biflorus L., its protoplast culture and their morphogenic potential. Curr. Sci. 55:447–452.

    Google Scholar 

  • Sinha RR, Das K & Sen SK (1983) Embryoids from mesophyll protoplasts of Vigna mungo L. Hepper, a seed legume crop plant. In: Plant Cell Culture in Crop Improvement (pp.209–214) Plenum Press, New York.

    Google Scholar 

  • Sree Ramulu K, Dijkhuis P, Roest S, Bokehnann GS & De Groot B (1984) Early occurrence of genetic instability in protoplast cultures of potato. Plant Sci. Lett. 36:79–86.

    Google Scholar 

  • Srinivasan C & Vasil IK (1985) Callus formation and plantlet regeneration from sugarcane protoplasts isolated from embryogenic cell suspension cultures. Am. J. Bot. 72:833.

    Google Scholar 

  • Sun JS, Lu TG & Sondahl MR (1989) Establishment and characterization of haploid suspension cells in supersweet maize. Acta Bot. Sinica 31:742–749.

    Google Scholar 

  • Tegeder M, Schieder O & Pickardt T (1995) Breakthrough in the plant regeneration from protoplasts of Vicia faba and V. narbonensiis. In: Terzimn et al. (eds.) Current Issues in Plant Molecular and Cellular Biology (pp:75–80) Kluwer Academic Publishers, Dordrecht.

    Google Scholar 

  • Terakawa T, Sato T & Koike M (1992) Plant regeneration from protoplasts isolated from embryogenic suspension cultures of creeping bentgrass (Agrostis palustris Huds.). Plant Cell Rep. 11:457–461.

    CAS  Google Scholar 

  • Thompson JA, Abdullah R & Cocking EC (1986) Protoplast culture of rice (Oryza sativa L.) using media solidified with agarose. Plant Sci. 47:123–133.

    Google Scholar 

  • Toriyama K, Hmata K & Sasaki T (1986) Haploid and diploid plant regeneration from protoplasts of anther callus in rice. Theor.Appl.Genet. 73:16–19.

    Google Scholar 

  • Tsai CK (1988) Plant regeneration from leaf callus protoplasts of Actinidia chinensis Planch. var. chinensis. Plant Sci. 54:231–235.

    Google Scholar 

  • Van Der Valk P & Zaal MACM (1988) Regeneration of plantlets from protoplasts of Poa pratensis L.(Kentucky bluegrass). In: Puite KJ et al. (eds.) Progress in Plant Protoplast Research (pp.59–60) Kluwer Academic Publishers, Dordrecht.

    Google Scholar 

  • Vardi A & Spiegel-Roy P (1982) Plant regeneration form Citrus protoplasts: variability in methodological requirements among cultivars and species. Theor.Appl.Genet 62:171–176.

    Google Scholar 

  • Vardi A, Hutchison DJ & Galun E (1986) A protoplast-to-tree system in Microcitrus based on protoplasts derived from a sustained embryogenic callus. Plant Cell Rep. 5:412–414.

    Google Scholar 

  • Vardi A, Spiegel-Roy P & Galun E (1975) Citrus cell culture: isolation of protoplasts, plating densities, effect of mutagens and regeneration of embryos. Plant Sci. Lett. 4:231–236.

    Google Scholar 

  • Vardi A, Spiegel-Roy P & Galun E (1983) Protoplast isolation, plant regeneration and somatic hybridization in different citrus species and microcitrus. In: Potrykus et al. (eds.) Protoplast 1983, Poster Proc. of 6th Intern. Protoplast Symp. Experientia(Suppl.) 45:284–285.

    Google Scholar 

  • Vasil IK & Vasil V (1992) Advances in cereal protoplast research. Physiol.Plant. 85:279–283.

    CAS  Google Scholar 

  • Vasil IK (1995) Cellualr and molecular genetic improvement of cereals. In: Terzi M et al. (eds.) Current Issues in Plant Molecular and Cellular Biology (pp.5–18) Kluwer Academic Publishers, Dordrecht.

    Google Scholar 

  • Vasil V & Vasil IK (1980) Isolation and culture of cereal protoplasts. Part.2: Embryogenesis and plantlet formation from protoplasts of Pennisetum americanum. Theor.Appl.Genet. 56:97–99.

    Google Scholar 

  • Vasil V & Vasil IK (1987) Formation of callus and somatic embryos from protoplasts of a commercial hybrid of maize (Zea mays L.). Theor.Appl. Genet. 73:793–798.

    Google Scholar 

  • Vasil V, Redway F & Vasil IK (1990) Regeneration of plants from embryogenic suspension culture protoplasts of wheat (Triticum aestivum L.). Bio/Technology 8:429–434.

    Google Scholar 

  • Vasil V, Wang DY & Vasil IK (1983) Plant regeneration from protoplasts of napier grass (Pennisetum purpureum Schum.). Z.Pflanzenphysiol. 111:233–239.

    CAS  Google Scholar 

  • Wang D, Miller PD & Sondahl MR (1989) Plant regeneration from protoplasts of Indica type rice and CMS rice. Plant Cell Rep. 8:329–332.

    Google Scholar 

  • Wang F, Shao XH & Chen RM (1992) Studies on protoplast culture and plant regeneration of Dimocarpus longan. Proc of the 1 st Symp. of Chinese Soc. of Agricultural Biotechnology (pp.109–112).

    Google Scholar 

  • Wang GY & Xia ZA (1987) Mature plant regeneration from rice protoplasts. Acta Biol.Exp.Sinica 20:253–257.

    Google Scholar 

  • Wang GY, Xia ZA & Wang LF (1986) Regenerated plantlets from cultured mesophyll protoplasts of onion (Allium cepa L.). Acta Bio,.Exp.Sinica 19:409–413.

    Google Scholar 

  • Wang SP, Xu ZH & Wei ZM (1991) Culture and regeneration of poplar mesophyll protoplasts. Sci.Sinica 34:587–592.

    CAS  Google Scholar 

  • Wang Y, Huang MR, Chen DM, Xu N, Wei ZM & Xu ZH (1995a) Plants regenerated from mesophyll protoplasts of cottonwood new clone. Acta Bot.Sinica 37:379–385.

    Google Scholar 

  • Wang Y, Huang MR, Wei ZM, Sun YR, Chen DM, Xu ZH, Zhang LM & Xu N (1995b) Regeneration of simon poplar (Populus simonii) from protoplast culture. Plant Cell Rep. 14:442–445.

    Google Scholar 

  • Wei ZM & Xu ZH (1988) Plant regeneration from protoplasts of soybean (Glycine max L.). Plant Cell Rep. 7:348–351.

    Google Scholar 

  • Wei ZM & Xu ZH (1989) Plant regeneration from Sorghum protoplast culture. Plant Physiol. Commic (Shanghai) 6:45–46.

    Google Scholar 

  • Wei ZM & Xu ZH (1990) Factors affecting culture of hypocotyl protoplasts of cauliflower and plantlet regeneration. Acta Phytophysiol.Sinica 16:394–400.

    Google Scholar 

  • Wei ZM & Xu ZH (1990) Protoplast culture and plant regeneration of soybean (Glycine max and G.soja). Acta Bot.Sinica 32:582–588.

    Google Scholar 

  • Wei ZM & Xu ZH (1990) Regeneration of fertile plants from embryogenic suspension culture protoplast of Sorghum vulgare. Plant Cell Rep. 9: 51–53.

    CAS  Google Scholar 

  • Wei ZM, Xu ZH, Huang JQ & Huang MR (1994) Plants regenerated from mesophyll protoplasts of white mulbeny. Cell Res. 4:183–189.

    Google Scholar 

  • Wei ZM, Xu ZH, Xu N & Huang MR (1991) Mesophyll protoplast culture and plant regeneration of oriental pinetree (Platanus orientalis). Acta Bot.Sinica 33:813–818.

    CAS  Google Scholar 

  • Wei ZM, Zhang Y & Xu ZH (1993) Plant regeneration iin peanut protoplast culture. Ann.Rep. of National Laboratory of Plant Mol.Genet.(1992). p.22–23.

    Google Scholar 

  • White DWR (1983) Plant regeneration from mesophyll protoplast of white clover (Trifolium repens L.). In: Potrykus I et al. (eds) Protoplast 1983, Poster Proc. of 6th Intern.Protoplast Symp.(pp.60–61) Birkhauser Verlag,Basel.

    Google Scholar 

  • Wilson VW, Haq N & Evans PK (1985) Protoplast isolation, culture and plant regeneration in the winged bean, Psophocarpus tetragonolobus (L.). Plant Sci. 41:61–68.

    CAS  Google Scholar 

  • Xia ZA (1992) Plant protoplast culture in China, Advaces in Science of China, Biology 3:77–86.

    Google Scholar 

  • Xiong XS, Jia SR & Fu YY (1988) Protoclonal variation in cabbage (Brassica oleracea L. var. capitata). Acta Horticult.Sinica 15:120–124.

    Google Scholar 

  • Xu XX & Xu ZH (1988) Plant regeneration from mesophyll protoplasts of Orychophragmus violaceus. Acta Phytophysiol. Sinica 14:170–174.

    Google Scholar 

  • Xu ZH, Davey NR & Cocking EC (1981) Isolation and sustained division of Phaseolus aureus (mung bean) root protoplasts. Z.Pflanzenphysiol. 104:289–298.

    Google Scholar 

  • Xu ZH & Wei ZM (1993) Protoplast culture of grain legumes. In: Soh WY et al. (eds.) Advances in Developmental Biology and Biotechnology of Higher Plants. (pp.197–209) Korean Soc. Plant Tissue Culture, Taejeon

    Google Scholar 

  • Xu ZH & Xue HW (1997) The species of plants regenerated from protoplasts of higher plants. In: Xu ZH & Wei ZM (eds.) Plant Protoplast Culture and Genetic Transformation. Chapter 3. Shanghai Sci. & Tech. Publ. P246.

    Google Scholar 

  • Xu ZH, Davey MR & Cocking EC (1982) Organogenesis from root protoplasts of the forage legumes Medicago sativa and Trigonella foenum-graecum Z. Pflanzenphysiol. 107:231–235.

    Google Scholar 

  • Xu ZH, Davey MR & Cocking EC (1984) Root protoplast isolation and culture in higher plants. Sci.Sinica (11):1012–1018.

    Google Scholar 

  • Xue HW, Wei ZM & Xu ZH (1994) Construction of high efficient regeneration system from hypocotyl protoplasts of Brassica oleracea. Acta.Biol.Exp.Sinica 27:259–269.

    Google Scholar 

  • Yamada Y, Yang ZQ & Tang DT (1986) Plant regeneration from protoplast-derived callus of rice (Oryza sanva L.). Plant Cell Rep. 5:85–88.

    Google Scholar 

  • Yamashita Y & Shimamoto K (1989) Regeneration of plants from cabbage (Brassica oleracea var. capitata) protoplasts. In: Bajajyps (ed) Biotechnology in Agriculture & Forestry, 8:193–205. Springer-Verlag, Berlin.

    Google Scholar 

  • Yan CQ, Bian ZX, Zhang DS, Yang JS & Ge KL (1991) Plantlet regeneration from mesophyll protoplasts of Canavallis ensiformis. J.Fudan Univer. (Natural Sci.) 30:381–386.

    Google Scholar 

  • Yan Q, Zhang X, Shi J & Li J (1990) Green plant regeneration from protoplasts of barley (Hordeum vulgare L.). Kexue Tongbao (20):1581–1583.

    Google Scholar 

  • Yan Q, Zhang X, Teng S, Huang C, Yan Q & Wang J (1995) Establishment of a system of culture technique from rice protoplasts. Acta Agric. Sinica,No2. Special issue on protoplast culture of field crops (pp.20–26).

    Google Scholar 

  • Yang JB, Wu JD, Wei ZM & Xu ZH (1992) Plant regeneration from protoplasts of Indica rece. Chinese J.Biotech. 8:60–64.

    Google Scholar 

  • Yang YM, He DD & Scott KJ (1993) Plant regeneration from protoplasts of dunum wheat (Tri ticum durum De st.cv. D6962). Plant Cell Rep. 12:320–323.

    Google Scholar 

  • Yang ZN & Xu ZH (1997) Protoplast culture of Cruciferous plants. In: Xu ZH & Wei ZM (eds.) Plant Protoplast Culture and Genetic Transformation. Chapter 12.Shanghai Sci. & Tech. Publ. (in Chinese) 124–141.

    Google Scholar 

  • Yang ZN, Xu ZH & Wei ZM (1994) Cauliflower inflorescence protoplast culture and plant regeneration. Plant Cell Tissue Organ Cult. 36:191–195.

    Google Scholar 

  • Yang ZQ, Shikanai K, Mori K & Yamada Y (1989) Plant regeneration from cytoplasmic hybrids of rice (Oryza sativa L.). Theor.Appl.Genet. 77:305–310.

    CAS  Google Scholar 

  • Yarrow SA & Barsby TL (1988) Recent advances in protoplast culture of horticultural crops: Vegetable crops. Sci.Horticult. 37:179–200.

    Google Scholar 

  • Yasugi S (1989) Isolation and culture of orchid protoplasts. In: Bajaj YPS (ed.) Biotechnology in Agriculture & Forestry 8:235–253. Springer, Berlin.

    Google Scholar 

  • Yin Y, Li S, Chen Y, Guo H, Tian W, Chen Y & Li L (1993) Fertile plants regenerated from suspension culture-derived protoplasts of an indica type rice (Oryza sativa L.). Plant Cell Tissue Organ Cult. 32:61–68.

    CAS  Google Scholar 

  • Zakri AH (1984) Plant regeneration from protoplast-derived callus of winged bean. In: Laned W et al. (eds.) Efficiency in Plant Breeding (pp.363) PUDOS,Wageningen.

    Google Scholar 

  • Zapata FJ & Sink KC (1981) Somatic embryogenesis from Lycopersicon peruvianum leaf mesophyll protoplasts. Theor.Appl.Genet. 59:265–268.

    Google Scholar 

  • Zhang GF, Huang BL, Wei L, Luo XM, Zheng XF, He MY & Hao S (1993) Plant regeneration from protoplasts derived from suspension cultures in Ceymus regemosus. Acta Bot.Sinica, 35:422–428.

    Google Scholar 

  • Zhang GF, Luo XM, Li FX & An LJ (1994) Plantlet regeneration of protoplasts derived from cell-suspension cultures of Oxytropis leptophylla. Acta Biol.Exp.Sinica, 27:117–121.

    CAS  Google Scholar 

  • Zhang H, Tian HQ & Li QM (1994) Plant regeneration from cell and protoplast cultures of Triticum aestivum-Haynaldia villosa hybrid. Acta Bot.Sinica, 36:479–482.

    Google Scholar 

  • Zhang YJ, Wu XJ, Cai QG, Zhou YL & Qian YQ (1995) Plantlet regeneration from leaf protoplasts in seedlings of Actinidia eriantha benth. Acta Bot.Sinica, 37:48–52.

    Google Scholar 

  • Zhang ZQ, Zhong WJ, Tang KX, Zhou Y & Zhu MF (1994) Plant regeneration from allotriploid (O.sativa X O.minuta) Hybrid protoplast. Acta.Crops.Sinica. 20: 578–581.

    Google Scholar 

  • Zhao GL & Luo XM (1990) Plant regeneration from protoplasts soybean (G4,ciine max L.). Acta Bot.Sinica, 32:616–627.

    Google Scholar 

  • Zhao GL (1990) Plant regeneration from saintoin (Onobrychis viciaefolia) protoplasts derived from cell suspensions of hypocotyl. Acta Bot.Sinica, 32:973–976.

    Google Scholar 

  • Zhao YX & Yao DY (1993) Plant regeneration from protoplasts isolated from callus tissue of Hibiscus syriacus L. Chinese J.Bot. 3:29–33.

    Google Scholar 

  • Zhao YX (1993) The isolation, culture and genetic manipulation of protoplast from salt and drought tolerant leguminous plants. Ph.D. Thesis, Coventy University, U.K.

    Google Scholar 

  • Zhao YX, Harris PJC & Yao DY (1995) Plant regeneration from protoplasts isolated from cotyledons of Sesbania bispinosa. Plant Cell Tissue Organ Cult. 40:119–123.

    Google Scholar 

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  1. National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, Shanghai, 200032, China

    Zhi-Hong Xu & Hong-Wei Xue

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Xu, ZH., Xue, HW. (1999). Plant Regeneration from Cultured Protoplasts. In: Soh, WY., Bhojwani, S.S. (eds) Morphogenesis in Plant Tissue Cultures. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9253-6_2

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