Abstract
Selective biosensor systems for determination of heavy metal ions based on ion sensitive field effect transistors and thin-film interdigitated planar (conductometric) electrodes have been developed. Glucose oxidase, alcohol oxidase and butyril cholinesterase immobilised on the transducer surfaces have been used as bioactive elements for preparation of Ag+, Hg2+ and Pb2+ sensitive biosensors. The biosensors developed exhibit a dynamic range of 1 – 100 μM for these heavy metal ions. The performance characteristics of the biosensors are discussed.
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References
Nikol’skaya E.B., Evtyugin G.A. and Shekhovtsova T.N. (1994) Problems and prospects the application of enzymes to environmental analysis, Journal of Analytical Chemistry (Russia) 49, 452–461.
Karube, I. (1988) Biosensing, Keigaku Publishing,Tokyo.
Guilbaut, G.G. and Mascini, M. (1988) Analytical Uses of Immobilized Biological Compounds for Detection, Medical and Technical Uses, D. Reidel Publishing. Co., Dordrecht.
Gayet, J-C., Haouz, A., Geleso-Meyer, A. and Burstein, C. (1993) Detection of heavy metal salts with biosensors built with an oxygen electrode coupled to varied immobilized oxidases and dehydrogenases, Biosensors and Bioelectronics 8, 177–183.
Zhylyak G.A., Korpan Y.1., Dzyadevich S.V., Soldatkin A.P. and El’skaya A.V. (1995) Application of urease conductometric biosensor for heavy metal ions determination, Sensors and Actuators B 24–25, 145–148.
Shul’ga, A.A., Netchiporouk, L.I., Sandrovsky, A.K., Abalov, A.A., Frolov, O.S., Kononenko, Yu..G., Maupas, H. and Martelet, C. (1995) Operation of an ISFET with non-insulated substrate directly exposed to the solution, Sensors and Actuators B 30, 101–105.
Soldatkin, A. P.; Shul’ga, A. A.; Martelet, C.; Jaffresic-Renault, N.; Maupas, H.; El’skaya, A. V. French Patent, # 93 05 941, May 12. 1993
Gibson, T.D. and Woodward, J.R. (1992) Protein stabilization in biosensor systems, in P.G. Edelman and J. Wang (eds.), Biosensors and Chemical Sensors, ACS, Washington, pp. 40–55.
Shul’ga, A.A., Sandrovsky, A.K., Strikha, V.I., Soldatkin, A.P., Starodub, N.F. and EI’skaya, A. V. (1992) Overall characterisation of ISFET-based glucose biosensor, Sensors and Actuators B 10, 41–46.
Dzyadevich, S.V., Shul’ga, A.A., Soldatkin, A.P., Nyamsi-Hendji, A.M., Jaffresic-Renault, N. and Martelet, C. (1994) Conductometric biosensors based on cholinesterases for sensitive detection of pesticides, Electroanalysis 6. 752–758.
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Soldatkin, A.P., Korpan, Y.I., Zhylyak, G.A., Martelet, C., El’Skaya, A.V. (1998). Selective Determination of Heavy Metal Ions with Sensors Coupled to Immobilised Enzymes. In: Nikolelis, D.P., Krull, U.J., Wang, J., Mascini, M. (eds) Biosensors for Direct Monitoring of Environmental Pollutants in Field. NATO ASI Series, vol 38. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-8973-4_26
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DOI: https://doi.org/10.1007/978-94-015-8973-4_26
Publisher Name: Springer, Dordrecht
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