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Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 70))

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Abstract

16S rRNA sequences were used to measure phylogenetic and evolutionary relationships among bacteria belonging to the alpha subdivision of the proteobacteria. Partial 16S rDNA sequences were determined for the five bacterial species belonging to the genus Azospirillum and compared with ribosomal sequences from other bacteria belonging to the alpha subdivision of the proteobacteria. The five Azospirillum species, A. lipoferum, A. brasilense, A. irakense, A. amazonense and A. halopraeferens, were grouped into a cluster which has been found to be related to Magnetospirillum magnetotacticum.

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References

  • Bally R, Thomas-Bauzon D, Heulin T, Balandreau J, Richard C, De Ley J 1983. Determination of the most frequent N2-fixing bacteria in a rice rhizosphere. Can. J. Microbiol., 29, 881–887.

    Google Scholar 

  • Bally R, Simonet P, Haurat J, Normand P 1992. Characterization of Azospirillum irakense and Azospirillum lipoferum by direct sequencing of a PCR amplified 16S rDNA gene. Symbiosis, 13, 47–53.

    CAS  Google Scholar 

  • Eden P A, Schmidt T M, Blakemore R P, Pace N R 1991. Phylogenetic analysis of Aquaspirillum magnetotacticum using polymerase chain reaction-amplified 16S rRNA specific DNA. Int. J. Syst. Bacteriol., 41, 324–325.

    Google Scholar 

  • Felsenstein J 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39, 783–791.

    Article  Google Scholar 

  • Higgins D G, Sharp P M 1988. Clustal: a package for performing multiple alignment on a microcomputer. Gene 73, 237–244.

    Article  PubMed  CAS  Google Scholar 

  • Jukes T H, Cantor C R 1969. Evolution of protein molecules. In: Munro H N, ed. Mammalian Protein Metabolism. Academic Press, New York, 21–132.

    Google Scholar 

  • Khammas K M, Ageron E, Grimont P A D, Kaiser P 1989. Azospirillum irakense sp. nov., a nitrogen-fixing bacterium associated with rice roots and rhizosphere soil. Res. Microbiol., 140, 679–693.

    Google Scholar 

  • Li W T 1981. Simple method for constructing phylogenetic trees from distance matrices. Proc. Natl. Acad. Sci. USA, 78, 1085–1089.

    Article  PubMed  CAS  Google Scholar 

  • Magalhaes F M M, Baldani, J I, Souto S M, Kuykendall J R, Döberreiner J 1983. A new acid tolerant Azospirillum species. An. Acad. Bras. Cienc., 55, 417–430.

    Google Scholar 

  • Maniatis T, Fritsch E F, Sambrook J 1982. Molecular Cloning, a Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

    Google Scholar 

  • Nazaret S, Cournoyer B, Normand P, Simonent P 1991. Phylogenetic relationships among Frankia genomic species determined by use of amplified 16S rDNA sequences. J Bacteriol., 173, 4072–4078. Nei M 1987. Molecular Evolutionary Genetics. Columbia University Press, New York.

    Google Scholar 

  • RauĂ© H A, Klootwijk J, Musters W 1988. Evolutionary conservation of structure and function of high molecular weight ribosomal RNA. Prog. Biophys. Mol. Biol., 51, 77–129.

    Google Scholar 

  • Reinhold B, Hurek T, Fendrik I. 1987. Azospirillum halopraeferens sp. nov., a nitrogen-fixing organism associated with roots of Kallar grass (Leptochloa fusca Kunth). Int. J. Syst. Bacteriol., 37, 43–51.

    Google Scholar 

  • Saitou N, Nei M 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol., 4, 406–425.

    Google Scholar 

  • Sanger F, Nicklen S, Coulson A R 1977. DNA sequencing with chain-terminating inhibitor. Proc. Natl. Acad. Sci. USA, 74, 5463–5467.

    Google Scholar 

  • Sneath P H A, Sokal R R 1973. Numerical Taxonomy. Freeman WH, San Francisco.

    Google Scholar 

  • Tarrand J J, Krieg N R, Döberreiner J 1978. A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov. Can. J. Microbiol., 24, 967–980.

    Google Scholar 

  • Winship P R 1989. An improved method for directly sequencing PCR amplified material using dimethyl sulfoxide. Nucl. Acids Res., 17, 1266.

    Article  PubMed  CAS  Google Scholar 

  • Woese C R 1987. Bacterial evolution. Microbiol. Rev., 51, 221–271.

    PubMed  CAS  Google Scholar 

  • Young J P W, Downer H L, Eardly B D 1991. Phylogeny of the phototrophic Rhizobium strain BTAi1 by polymerase chain reaction-based sequencing of a 16S rRNA gene segment. J. Bacteriol., 173, 2271–2277.

    PubMed  CAS  Google Scholar 

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© 1996 Springer Science+Business Media Dordrecht

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Bally, R., Kabir, M., Rahman, M., Haurat, J., Normand, P. (1996). Azospirillum phylogeny based on 16S rRNA sequences. In: Rahman, M., Podder, A.K., Van Hove, C., Begum, Z.N.T., Heulin, T., Hartmann, A. (eds) Biological Nitrogen Fixation Associated with Rice Production. Developments in Plant and Soil Sciences, vol 70. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-8670-2_24

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  • DOI: https://doi.org/10.1007/978-94-015-8670-2_24

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-4742-7

  • Online ISBN: 978-94-015-8670-2

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