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Engineering aspects of plant propagation in bioreactors

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Automation and environmental control in plant tissue culture

Abstract

Millions of plants are produced every year through micropropagation worldwide. However, these methods are labour intensive and there has to be a good reason for choosing micropropagation. Either the crop is so valuable in itself, or the product from micropropagation is of superior quality and therefore able to obtain a higher price. Or, traditional propagation is so costly, difficult or impossible that micropropagation solves a problem (Hvoslef-Eide 1987; Aitken-Christie 1991). Jones and Sluis (1991) claim that price is the single greatest barrier to true exploitation of mass production opportunities. Automation of somatic embryogenesis/organogenesis in bioreactors has been advanced by several authors, among them Styer (1985), Preil et al. (1988) and Levin et al. (1988), as a possible way of reducing the labour costs of micropropagation. Bioreactors have traditionally been used for bacterial fermentation or for large scale production of secondary metabolites from plant cells. Growing plants cells for production of somatic embryos in bioreactors designed for bacterial growth and production of secondary metabolites is not as straightforward as first thought. Experience from a European collaboration on regeneration from suspension cultures has lead us to believe that the shear forces from fast propellers and foam formation in bubble aerated reactors seem to have caused most trouble.

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Authors and Affiliations

  1. Department of Agricultural Engineering, Agricultural University of Norway, P.O. Box 5065, N-1432, Ås, Norway

    Petter Hieronymus Heyerdahl & Odd Arild Sollien Olsen

  2. Department of Horticulture and Crop Sciences, Agricultural University of Norway, P.O. Box 5022, N-1432, Ås, Norway

    Anne Kathrine Hvoslef-Eide

Authors
  1. Petter Hieronymus Heyerdahl
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  2. Odd Arild Sollien Olsen
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  3. Anne Kathrine Hvoslef-Eide
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Editor information

Editors and Affiliations

  1. Forest Biotechnology Centre, Carter Holt Harvey Forestry Group, Rotorua, New Zealand

    Jenny Aitken-Christie

  2. Faculty of Horticulture, Chiba University, Matsudo, Chiba, Japan

    Toyoki Kozai

  3. Plant Sciences Lab, University of Illinois, Urbana, Illinois, USA

    Mary Ann Lila Smith

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© 1995 Springer Science+Business Media Dordrecht

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Heyerdahl, P.H., Olsen, O.A.S., Hvoslef-Eide, A.K. (1995). Engineering aspects of plant propagation in bioreactors. In: Aitken-Christie, J., Kozai, T., Smith, M.A.L. (eds) Automation and environmental control in plant tissue culture. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-8461-6_5

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  • DOI: https://doi.org/10.1007/978-94-015-8461-6_5

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-4405-1

  • Online ISBN: 978-94-015-8461-6

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