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Low temperature storage of plant tissue cultures

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Automation and environmental control in plant tissue culture

Abstract

Micropropagation is firmly established as a global technology for plant production systems and is widely used in teaching and research as well as, on the larger scale, for commercial production. It is extremely labour-intensive for a modern technology, as the interval between subcultures may range, typically, from 14 to 35 days. This creates a high, continual demand for skilled labour, media, culture vessels and space in the specialised environmental conditions required for optimal growth. In any micropropagation facility, whatever its size, there will be a high proportion of material being held for some future use, and which is not part of a current multiplication or experimental programme. Consequently, there will be significant logistic and resource benefits in employing a storage system for this material, to extend significantly the intervals between subculture and other handling. Resource savings will also be made if the storage system can operate at lower temperatures and light levels than those required for optimal growth. Programmed use of such a storage system would also allow greater control of production schedules for micropropagated material, with efficient stockpiling of multiplying cultures or plantlets.

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  1. Crisp Innovar Ltd., Glebe House, Station Road, Reepham, Norfolk, NR10 4NB, UK

    Brian W. W. Grout

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  1. Forest Biotechnology Centre, Carter Holt Harvey Forestry Group, Rotorua, New Zealand

    Jenny Aitken-Christie

  2. Faculty of Horticulture, Chiba University, Matsudo, Chiba, Japan

    Toyoki Kozai

  3. Plant Sciences Lab, University of Illinois, Urbana, Illinois, USA

    Mary Ann Lila Smith

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© 1995 Springer Science+Business Media Dordrecht

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Grout, B.W.W. (1995). Low temperature storage of plant tissue cultures. In: Aitken-Christie, J., Kozai, T., Smith, M.A.L. (eds) Automation and environmental control in plant tissue culture. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-8461-6_21

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  • DOI: https://doi.org/10.1007/978-94-015-8461-6_21

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