Abstract
The storage triacylglycerols of oil-rich seeds are hydrolyzed during germination of such seeds by the action of the endogenous lipase (triacylglycerol acylhydrolases, EC 3.1.1.3) and the lipolysis products, i. e. fatty acids and glycerol, are metabolized further in glyoxysomes and other organelles. It is well known that germinating seedlings of many oilseeds are rich source of lipases which have been isolated, purified and characterized from such tissues and some of these purified enzyme preparations have been used as catalyst in biotransformation of lipids [1,2]. Especially, the lipase from germinating seedlings of the very common oilseed — rape (Brassica napus L.) — has been isolated [3,4] partially purified [5,6] and used as biocatalyst after immobilization [7–10].
Part of the doctoral thesis of Iván Jachmanián to be submitted to Facultad de Quimica, Universidad de la República, Montevideo, Uruguay.
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References
Mukherjee KD. Plant lipases and their application in lipid biotransformations. Prog Lipid Res 1994; 33:165–174.
Mukherjee KD, Hills MJ. Lipases from plants. In: Woolley P, Petersen SB, editors. Lipases — their structure, biochemistry and application. Cambridge: Cambridge University Press, 1994:49–75.
Hassanien FR, Mukherjee KD. Isolation of lipase from germinating oilseeds for biotechnological processes. J Am Oil Chem Soc 1986; 63:893–897.
Theimer RR, Rosnitschek I. Development and intracellular localization of lipase activity in rapeseed (Brassica napus L.) cotyledons. Planta 1978; 139:249–256.
Hills MJ, O’Sullivan JN. Partial purification of lipase from cotyledons of oilseed rape (Brassica napus L.). Biochem Soc Trans 1989; 17:480.
Weselake RJ, Thomson LW, Tenaschuk D, Mackenzie SL. Properties of a solubilized microsomal lipase from germinating Brassica napus. Plant Physiol 1989;91:1303–1307.
Hills MJ, Mukherjee KD. Triacylglycerol lipase from rape (Brassica napus L.) suitable for biotechnological purposes. Appl Biochem Biotechnol 1990; 26:1–10.
Hills MJ, Kiewitt I, Mukherjee KD. Synthetic reactions catalyzed by immobilized lipase from oilseed rape (Brassica napus L.). Appl Biochem Biotechnol 1991; 27:123–129.
Hills MJ, Kiewitt I, Mukherjee KD. Lipase from Brassica napus L. discriminates against cis-4 and cis-6 unsaturated fatty acids and secondary and tertiary alcohols. Biochim Biophys Acta 1990; 1042:237–240.
Hills MJ, Kiewitt I, Mukherjee KD. Enzymatic fractionation of evening primrose oil by rape lipase: Enrichment of γ-linolenic acid. Biotechnol Lett 1989; 11:629–632.
Mukherjee KD, Kiewitt I, Hills MJ. Substrate specificity of lipases in view of kinetic resolution of unsaturated fatty acids. Appl Microbiol Biotechnol 1993; 40:489–493.
Rangheard M-S, Langrand G, Triantaphylides C, Baratti J. Multi-competitive enzymatic reactions in organic media: a simple test for the determination of lipase fatty acid specificity. Biochim Biophys Acta 1989; 1004:20–28.
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© 1995 Springer Science+Business Media Dordrecht
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Mukherjee, K.D., Jachmanián, I. (1995). Germinating Rapeseed as Biocatalyst for Lipolytic and Synthetic Reactions. In: Kader, JC., Mazliak, P. (eds) Plant Lipid Metabolism. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-8394-7_153
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DOI: https://doi.org/10.1007/978-94-015-8394-7_153
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