Abstract
The formation of glycerol-3-phosphate (G3P) in plants as a substrate for glycerolipid biosynthesis can be accomplished by either a glycerol-3-phosphate dehydrogenase (GPDH, EC 1.1.1.8) or a glycerol kinase (GK). However, it is likely that GPDH rather than GK is responsible for the major part of G3P formation, since GPDH the activity of GK is particularly low in oil-synthesizing plant tissues [1]. Two highly regulated isoforms of GPDH, one plastidic and the other cytosolic, have been described [2]. In order to investigate the role of GPDH in glycerolipid biosynthesis a cDNA encoding GPDH was isolated from an expression library of immature embryos from Cuphea lanceolata [3] by functional complementation of an Escherichia coli (E. coli) G3P auxotroph. The cDNA was completely sequenced and the longest open reading frame (ORF) consisting of 372 codons was found to share about 50% amino acid identity with animal GPDH sequences.
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© 1995 Springer Science+Business Media Dordrecht
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Hausmann, L., Schell, J., Töpfer, R. (1995). Cloning of a cDNA Coding for a Glycerol-3-Phosphate Dehydrogenase from Cuphea Lanceolata . In: Kader, JC., Mazliak, P. (eds) Plant Lipid Metabolism. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-8394-7_148
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DOI: https://doi.org/10.1007/978-94-015-8394-7_148
Publisher Name: Springer, Dordrecht
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