Abstract
Resistant potato clones were separated from susceptible ones by dual in vitro culture of Phytophthora infestans (Mont.) de Bary and micropropagated plantlets. This test was applied to plantlets of cv. Judith (a new promising belgian variety) regenerated from internode calli, using a regeneration medium containing either zeatin or 2iP. A collection of 2,500 calliclones were inoculated with a suspension of P. infestans sporangia; differences in resistance were observed only in plants regenerated from zeatin containing medium.
Some 68 resistant calliclones were micropropagated and their progeny was inoculated in vitro with sporangia of the late blight fungus; 90 % of this material exhibited in vitro resistance compared to 100 % susceptibility for the parent cv. Judith. Resistant clones were transferred to the nursery for further studies, and duplicates were planted at Libramont (Belgian Ardennes) to assess their possible field resistance.
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© 1986 Springer Science+Business Media Dordrecht
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Meulemans, M., Duchene, D., Tegera, P., Fouarge, G. (1986). Selection of Variants by Dual Cultures of Potato and Phytophthora Infestans. In: Somaclonal Variations and Crop Improvement. Advances in Agricultural Biotechnology, vol 20. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-7733-5_22
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DOI: https://doi.org/10.1007/978-94-015-7733-5_22
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