Summary
Hybrid clones and subclones between human lymphoblastoid FV5 cells and MCB2 fibroblasts were examined for the presence of Epstein-Barr virus (EBV) DNA, the expression of the virus-determined nuclear antigen (EBNA), and the presence of human chromosomes, in the course of serial passage in vitro. Among 14 hybrid clones examined, three were positive for EBNA and EBV DNA and had human chromosome 14, but the remaining 11 clones were totally negative for both EBV markers and did not reveal the presence of this particular chromosome. Furthermore, ten subclones isolated from one of the three positive clones contained only chromosome 14 as the human chromosome. In all of these subclones EBNA, EBV DNA and chromosome 14 were segregated concordantly. These results seem to indicate an apparent linkage relationship between human chromosome 14 and the EBNA antigen gene in human lymphoblastoid cells.
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Yamamoto, K., Mizuno, F., Matsuo, T., Tanaka, A., Nonoyama, M., Osato, T. (1981). Assignment of Epstein-Barr Virus-determined Nuclear Antigen (EBNA) Gene to Chromosome 14 in Human Lymphoblastoid Cells. In: Becker, Y. (eds) Herpesvirus DNA. Developments in Molecular Virology, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-6897-5_16
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DOI: https://doi.org/10.1007/978-94-015-6897-5_16
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