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Some applications of quantitative two-dimensional immunoelectrophoresis in the study of the systemic acute-phase reaction of the rat

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Perspectives in Inflammation

Part of the book series: Future Trends in Inflammation ((FTIN,volume 3))

Abstract

By means of two-dimensional immunoelectrophoresis according to the method of Clarke and Freeman1 it is possible to determine quantitatively at least 30 immunologically distinct rat serum proteins’. Fifteen of these proteins were identified by specific staining methods, physicochemical parameters or biochemical functions (Table 39.1).

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References

  1. Clarke, M. H. G. and Freeman, T. (1968). Quantitative immunoelectrophoresis of human serum proteins. Clin. Sci. 35, 403.

    PubMed  CAS  Google Scholar 

  2. DiRosa, M. D. (1972). Biological properties of carrageenan. J. Pharm. Pharmacol., 24, 89

    CAS  Google Scholar 

  3. Abd-El-Fattah, M., Scherer, R. and Ruhenstroth-Bauer, G. (1976). Application of quantitative two-dimensional immunoelectrophoresis in the study of the acute-phase reaction following injection of the lipid A component of bacterial lipopolysaccharides in rats. J. Mol. Med. 1, 211.

    CAS  Google Scholar 

  4. Billingham, M. E. and Gordon, A. H. (1975). The role of the acute phase reaction in inflammation. In: Future Trends in Inflammation II (J.-P. Giroud, D. A. Willoughby and G. P. Velo, eds.) pp. 195–200. ( Basel and Stuttgart: Birkhäuser Verlag )

    Google Scholar 

  5. Pekarek, R., Wannemacher, R., Powanda, M., Abeles, F., Mosher, D., Dintermann, R. and Beisel, W. (1974). Further evidence that leukocytic endogenous mediator (LEM) is not endotoxin. Life Sci., 14, 1765

    Article  PubMed  CAS  Google Scholar 

  6. Pekarek, R., Wannemacher, R. W., Chapple, F. E., Powanda, M. C. and Beisel, W. (1972). Further characterization and species specificity of leukocytic endogenous mediator (LEM). Proc. Soc. Exp. Biol. Med., 141, 643

    PubMed  CAS  Google Scholar 

  7. Kampschmidt, R. F. and Upchurch, H. F. (1974). Effect of leukocytic endogenous mediator on plasma fibrinogen and haptoglobin. Proc. Soc. Exp. Biol. Med., 146, 904

    PubMed  CAS  Google Scholar 

  8. Eddington, C. L., Upchurch, H. F. and Kampfschmidt, R. F. (1972). Quantitation of plasma a-2-AP globulin before and after stimulation with leukocytic extracts. Proc. Soc. Exp. Biol. Med., 139, 565

    PubMed  CAS  Google Scholar 

  9. Pekarek, R. S., Powanda, M. C. and Wannemacher, R. W. (1972). The effect of leukocytic endogenous mediator on serum copper and coeruloplasmin concentrations in the rat. Proc. Soc. Exp. Biol. Med., 141, 1029

    PubMed  CAS  Google Scholar 

  10. Beisel, W. R. (1975). Metabolic response to infection. In: Annual Review of Medicine (W. P. Creger, C. H. Coggins and E. W. Hancock, eds.) Vol. 26, pp. 9–20

    Google Scholar 

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Scherer, R., Abd-El-Fattah, M., Ruhenstroth-Bauer, G. (1977). Some applications of quantitative two-dimensional immunoelectrophoresis in the study of the systemic acute-phase reaction of the rat. In: Willoughby, D.A., Giroud, J.P., Velo, G.P. (eds) Perspectives in Inflammation. Future Trends in Inflammation, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-7185-4_39

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  • DOI: https://doi.org/10.1007/978-94-011-7185-4_39

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-011-7187-8

  • Online ISBN: 978-94-011-7185-4

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