Skip to main content
SpringerLink
Log in
Book cover

Perspectives in Inflammation pp 211–223Cite as

Matrix vesicles and apatite nodules in arthritic cartilage

  • Chapter

Part of the book series: Future Trends in Inflammation ((FTIN,volume 3))

Abstract

Early ultrastructural observations of rabbit articular cartilage had shown a pericellular distribution of electron-dense lipidic debris in the matrix surrounding the chondrocytesl. The debris was found to contain rounded membranous bodies (up to 200 nm in diameter) which sometimes had a granular appearance and their accumulation in articular cartilage was age-related. Both fibrillated and non-fibrillated specimens of human articular cartilage showed similar electron-dense particles surrounding healthy cells and it was suggested that these were derived from cytoplasmic processes extending from the chondrocyte into the surrounding matrix2,3. Meachim4 found them in human osteoarthrotic articular cartilage, and their distribution appeared similar to that found for phospholipid and neutral lipid by light microscopic histochemistry5. Similar vesicular bodies were seen in other studies of articular cartilage and they have been variously described as cellular debris6, Corona vesicles7, matrix granules8, membranous debris9 and lipid material10, and extracellular lipid11. The function of these peri-cellular bodies has not been satisfactorily explained. Initially Ghadially et al. 2 considered these osmiophilic bodies to consist of physiologically extruded lipid from the cells. Chrisman, Semonsky and Beusch12, using electron histochemical methods, showed the presence of esterase and cathepsin-type of activity in the extracellular particles in articular cartilage and thought of them as lysosomes. Bonner and Owen13 in an abstract mentioned that these bodies stained with lipid stains and at electron microscopic level reacted with substrates for acid phosphatase, β-glucuronidase and N-acetyl-β-glucosaminidase. They too postulated them as extracellular lysosomes but none of the pictures or evidence has been published in detail. Thyberg and Friberg14 using epiphyseal cartilage showed some acid phosphatase and aryl sulphatase activity to be associated with extracellular vesicles.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barnett, C. H., Cochrane, W. and Palfrey, A. J. (1963). Age changes in articular cartilage of rabbits. Ann. Rheum. Dis., 22, 389

    Article  PubMed  CAS  Google Scholar 

  2. Ghadially, F. N., Meachim, G. and Collins, D. H. (1965). Extracellular lipid in the matrix of human articular cartilage. Ann. Rheum. Dis., 24, 136

    Article  PubMed  CAS  Google Scholar 

  3. Ghadially, F. N. and Roy, S. (1969). Ulstrastructure of Synovial Joints in Health and Disease. ( London: Butterworths )

    Google Scholar 

  4. Meachim, G. (1969). Age and osteoarthritic changes in articular cartilage. In: Ultra-structure of Synovial Joints in Health and Disease. ( F. N. Ghadially and S. Roy, eds.) p. 61. ( London: Butterworths )

    Google Scholar 

  5. Stockwell, R. A. (1965). Lipid in the matrix of ageing articular cartilage. Nature (London), 207, 427

    Article  CAS  Google Scholar 

  6. Rüttner, J. R. and Spycher, M. A. (1968). Electron microscopic investigations on aging and osteoarthrotic human cartilage. Pathol. Microbiol., 31, 14

    Google Scholar 

  7. Spycher, M. A., Moor, H. and Ruettner, J. R. (1969). Electron microscopic investigations of aging and osteoarthrotic human articular cartilage, II. The fine structure of freeze-etched aging hip joint cartilage. Z. Zellforsch., 98, 512

    Article  PubMed  CAS  Google Scholar 

  8. Zimny, M. L. and Redler, I. (1969). An ultrastructural study of patellar chondromalacia in humans. J. Bone Jt. Surg., 51-A, 1179

    Google Scholar 

  9. Ghadially, F. N., Fuller, J. A. and Kirkaldy-Willis, W. H. (1971). Ultrastructure of full-thickness defects in articular cartilage. Arch. Pathol., 92, 356

    PubMed  CAS  Google Scholar 

  10. Silberberg, R. and Hasler, M. (1969). Electron microscopy of articular cartilage: the effect of acute hyperthyroidism. Pathol. Microbiol., 31, 25

    Google Scholar 

  11. Weiss, C. and Mirow, S. (1972). An ultrastructural study of osteoarthritic changes in the articular cartilage of human knees. J. Bone Jt. Surg., 54-A, 954

    Google Scholar 

  12. Chrisman, O. D., Semonsky, C. and Beusch, K. G. (1967). Cathepsins in articular cartilage. In: The Healing of Osseous Tissue. (R. A. Robinson, ed.). p. 169. ( Washington D.C.: National Academy of Science and National Research Council Publications )

    Google Scholar 

  13. Bonner, Jr., W. M. and Owen, C. (1968). Hydrolytic enzymes in extracellular granules of aging articular cartilage. Arthritis Rheum, 11, 816

    Google Scholar 

  14. Thyberg, J. and Friberg, U. (1972). Electron microscopic enzyme histochemical studies on the cellular genesis of matrix vesicles in the epiphyseal plate. J. Ultrastruct. Res., 41, 43

    Article  PubMed  CAS  Google Scholar 

  15. Ali, S. Y. and Evans, L. (1973). Enzymic degradation of cartilage in osteoarthritis, Fed. Proc., 32, 1494

    PubMed  CAS  Google Scholar 

  16. Ali, S. Y. and Bayliss, M. T. (1974). Enzymic changes in human osteoarthrotic cartilage, In S. Y. Ali, D. W. Elves and D. H. Leabech (eds.) Normal and Osteoarthrotic Articular Cartilage, p. 189. ( London: Institute of Orthopaedics Publication )

    Google Scholar 

  17. Ali, S. Y. and Wisby, A. (1975). Ulstrastructural aspects of normal and osteoarthrotic cartilage, Ann. Rheum. Dis., 34, Suppl. 2, 21

    Google Scholar 

  18. Ali, S. Y. and Wisby, A. (1976). The role of matrix vesicles in human osteoarthrotic cartilage. Proc. Roy. Mic. Soc., 11, Pt. 5, 62

    Google Scholar 

  19. Bonucci, E. (1967). Fine structure of early cartilage calcification. J. Ultrastruct. Res., 20, 33

    Article  PubMed  CAS  Google Scholar 

  20. Anderson, H. C. (1969). Vesicles associated with calcification in the matrix of epiphyseal cartilage. J. Cell. Biol., 41, 59

    Article  PubMed  CAS  Google Scholar 

  21. Anderson, H. C. (1976). Matrix vesicles of cartilage and bone. In: The Biochemistry and Physiology of Bone, ( G. H. Bourne, ed.) Vol. IV, p. 135. ( New York: Academic Press )

    Google Scholar 

  22. Matsuzawa, L and Anderson, H. C. (1971). Phosphatases of epiphyseal cartilage studied by electron microscopic cytochemical methods. J. Histochem. Cyotchem.,19 801

    Google Scholar 

  23. Ali, S. Y., Sajdera, S. W. and Anderson, H. C. (1970). Isolation and characterization of calcifying matrix vesicles from epiphyseal cartilage. Proc. Nat. Acad. Sci. U.S., 67, 1513

    Article  CAS  Google Scholar 

  24. Ali, S. Y. (1976). Analysis of matrix vesicles and their role in the calcification of epiphyseal cartilage. Fed. Proc., 35, 135

    PubMed  CAS  Google Scholar 

  25. MATRIX VESICLES AND APATITE NODULES

    Google Scholar 

  26. Ali, S. Y. and Evans, L. (1973). The uptake of (45Ca) calcium ions by matrix vesicles isolated from calcifying cartilage, Biochem. J., 134, 647

    PubMed  CAS  Google Scholar 

  27. Ali, S. Y., Wisby, A., Evans, L. and Craig-Gray, J. (1977). The sequence of calcium and phosphorus accumulation by matrix vesicles. Calc. Tiss. Res.,(In press)

    Google Scholar 

  28. Johnson, L. C. (1962). Joint remodelling as the basis for osteoarthritis. J. Am. Vet. Med. Assoc., 141, 1237

    Google Scholar 

  29. Gersh, I. and Catchpole, H. R. (1960). The nature of ground substance of connective tissue. Perspec. Biol. Med., 3, 282

    CAS  Google Scholar 

  30. Sokoloff, L. (1963). Elasticity of articular cartilage: effect of ions and viscous solutions. Science, 141, 1055

    Article  PubMed  CAS  Google Scholar 

  31. McCarty, D. J. (1970). Crystal deposition diseases. In: Disease-a-Month. (H. F. Dowling, ed.) p. 5. ( Chicago: Year Book Medical Publishers Inc. )

    Google Scholar 

  32. McCarty, D. J. and Gatter, R. A. (1966). Recurrent acute inflammation associated with focal apatite crystal deposition. Arthritis Rheum, 9, 804

    Article  PubMed  Google Scholar 

  33. Dieppe, P. A., Huskisson, E. C., Crocker, P. and Willoughby, D. A. (1976). Apatite deposition disease, a new arthropathy. Lancet, i, 266

    Google Scholar 

  34. Reginato, A. J., Schumacher, H. R. and Martinez, V. A. (1974). The articular cartilage in familial chondrocalcinosis, light and electron microscopic study. Arthritis Rheum, 17, 977

    Google Scholar 

  35. Howell, D. S., Muniz, O. and Pita, J. C. (1974). Extrusion of pyrophosphate into extracellular media by cartilage incubates. In: Normal and Osteoarthrotic Articular Cartilage. ( S. Y. Ali, M. W. Elves and D. H. Leaback, eds.) p. 177 ( London: Institute of Orthopaedics Publication )

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. UK

    S. Yousuf Ali

Authors
  1. S. Yousuf Ali
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Experimental Pathology and Rheumatology, St. Bartholomew’s Hospital, London E.C.1, UK

    D. A. Willoughby (Professor) (Professor)

  2. Department of Pharmacology, Hôpital Côchin, Paris 14, France

    J. P. Giroud (Professor) (Professor)

  3. Department of Pharmacology, Policlinico Borgo, I-37100, Rome, Verona, Italy

    G. P. Velo (Professor) (Professor)

Rights and permissions

Reprints and permissions

Copyright information

© 1977 MTP Press Limited

About this chapter

Cite this chapter

Ali, S.Y. (1977). Matrix vesicles and apatite nodules in arthritic cartilage. In: Willoughby, D.A., Giroud, J.P., Velo, G.P. (eds) Perspectives in Inflammation. Future Trends in Inflammation, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-7185-4_15

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-7185-4_15

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-011-7187-8

  • Online ISBN: 978-94-011-7185-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation