Skip to main content
SpringerLink
Log in

Investigation of Type 1 Copper Site Geometry by Spectroscopy and Molecular Redesign

  • Chapter
Bioinorganic Chemistry of Copper

Abstract

Resonance Raman (RR) spectra of type 1 Cu proteins (cupredoxins) show a multiplicity of vibrational fundamentals between 250 and 500 cm-1 that is ascribed to kinematic coupling of the Cu-S(Cys) stretch with deformations of the Cys and His ligand side chains. A similar set of vibrational frequencies is observed for 11 different cupredoxins. These findings suggest that all cupredoxins have a highly conserved Cu(His)2Cys geometry including (i) a trigonal planar array for the three Cu ligands and (ii) a coplanar arrangement of the Cu-S-C ß-Cα-N atoms in the Cu-cysteinate moiety.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. E. T. Adman, Adv. Protein Chem. 45, 145 (1991).

    Article  Google Scholar 

  2. E. T. Adman, in Metalloproteins, P. M. Harrison, Ed., (Verlag Chemie, Weinheim, 1985), Part I, pp. 1–42.

    Google Scholar 

  3. V. A. Roberts, H. C. Freeman, A. J. Olsen, J. A. Tainer, and E. D. Getzoff,J.Biol Chem. 266, 13431 (1991).

    PubMed  CAS  Google Scholar 

  4. L. Chen, R. Durley, B. J. Poliks, K. Hamada, Z. Chen, F. S. Mathews, V. L. Davidson, Y. Satow, E. Huizinga, F. M. D. Vellieux, and W. G. J. Hol, Biochemistry 31, 4959 (1992).

    Article  PubMed  CAS  Google Scholar 

  5. J. W. Godden, S. Turley, D. C. Teller, E. T. Adman, M. Y. Liu, W. J. Payne, and J. LeGall, Science 253, 438 (1991).

    Article  PubMed  CAS  Google Scholar 

  6. A. Messerschmidt, R. Ladenstein, R. Huber, M. Bolognesi, L. Avigliano, R. Petruzelli, A Rossi, and A Finazzi-Agro,J.Mol. Biol. 224, 179 (1992).

    Article  PubMed  CAS  Google Scholar 

  7. J. Han, E. T. Adman, T. Beppu, R. Codd, H. C. Freeman, L. Huq, T. M. Loehr, and J. Sanders-Loehr, Biochemistry 30, 10904 (1991).

    Article  PubMed  CAS  Google Scholar 

  8. E. I. Solomon, M. J. Baldwin, and M. D. Lowery, Chem. Rev. 92, 521 (1992).

    Article  CAS  Google Scholar 

  9. E. W. Ainscough, A.G. Bingham, A.M. Brodie, W. R. Ellis, H. B. Gray, T. M. Loehr, J. E. Plowman, G. E. Norris, and E. N. Baker, Biochemistry 26, 71 (1987).

    Article  PubMed  CAS  Google Scholar 

  10. K. D. Sharma, T. M. Loehr, J. Sanders-Loehr, M. Husain, and V. L. Davidson,J.Biol Chem. 263, 3303 (1988).

    PubMed  CAS  Google Scholar 

  11. T. J. Thamann, P. Frank, L. J. Willis, and T. M. Loehr, Proc. Natl. Acad. Sci. U.S.A. 79, 6396 (1982).

    Article  PubMed  CAS  Google Scholar 

  12. L. Nestor, J. A. Larrabee, G. Woolery, B. Reinhammar, and T. G. Spiro, Biochemistry 23, 1084 (1984).

    Article  CAS  Google Scholar 

  13. D. F. Blair, G. W. Campbell., J. R. Schoonover, S. I. Chan, H. B. Gray, B. G. Malmström, I. Pecht, B. I. Swanson, W. H. Woodruff, W. K. Cho, A. M. English, H. A. Fry, V. Lum, and K. A Norton,J.Am. Chem. Soc. 107, 5755 (1985).

    Article  CAS  Google Scholar 

  14. T. den Blaauwen, M. van de Kamp, and G. W. Canters,J.Am. Chem. Soc. 113, 5050 (1991).

    Article  Google Scholar 

  15. T. den Blaauwen, G. W. Canters, J. Han, T. M. Loehr, and J. Sanders-Loehr, manuscript in preparation.

    Google Scholar 

  16. S. Han, R. S. Czernuszewicz, and T. G. Spiro,J.Am. Chem. Soc. 111, 3496 (1989).

    Article  CAS  Google Scholar 

  17. W. Maret, A. K. Shiemke, W. D. Wheeler, T. M. Loehr, and J. Sanders-Loehr,J.Am. Chem. Soc. 108, 6351 (1986).

    Article  CAS  Google Scholar 

  18. P. R. Carey in Biochemical Applications of Raman and Resonance Raman Spectroscopies (Academic Press, New York, 1982) p. 47.

    Google Scholar 

  19. J. Han, T. M. Loehr, Y. Lu, J. S. Valentine, B. A. Averill, and J. Sanders-Loehr, manuscript submitted.

    Google Scholar 

  20. Y. Lu, E. B. Gralla, J. A Roe, and J. S. Valentine,J.Am. Chem. Soc. 114, 3560 (1992).

    Article  CAS  Google Scholar 

  21. A. A. Gewirth, S. L. Cohen, H. J. Schugar, and E. I. Solomon, Inorg. Chem. 26, 1133 (1987).

    Article  CAS  Google Scholar 

  22. A. Romero, C. W. G. Hoitink, H. Nar, R. Huber, A. Messerschmidt, and G. W. Canters, manuscript submitted.

    Google Scholar 

  23. N. Kitajima, K. Fujisawa, M. Tanaka, and Y. Moro-oka, manuscript submitted.

    Google Scholar 

  24. T. den Blaauwen and G. W. Canters, manuscript submitted.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical and Biological Sciences, Oregon Graduate Institute of Science & Technology, Beaverton, OR, 97006-1999, USA

    Joann Sanders-Loehr

Authors
  1. Joann Sanders-Loehr
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. The Johns Hopkins University, USA

    Kenneth D. Karlin  & Zoltán Tyeklár  & 

Rights and permissions

Reprints and permissions

Copyright information

© 1993 Chapman & Hall, Inc.

About this chapter

Cite this chapter

Sanders-Loehr, J. (1993). Investigation of Type 1 Copper Site Geometry by Spectroscopy and Molecular Redesign. In: Karlin, K.D., Tyeklár, Z. (eds) Bioinorganic Chemistry of Copper. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-6875-5_4

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-6875-5_4

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-011-6877-9

  • Online ISBN: 978-94-011-6875-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation