Abstract
IN THIS CHAPTER THE MAJOR PHYSICAL METHODS THAT ARE APPLICABLE TO the study of membranes will be outlined. It is not possible in the space available either to treat fully the theoretical basis of the methods or to give a comprehensive coverage of their applications. Accordingly, the principles of the methods will be dealt with, only in so far as is necessary to indicate the physical significance of the parameters that are measured in membrane studies. The examples of application of the methods are chosen so as to give an idea of the range of information that can be obtained by use of each technique, and to indicate the potential and relative values of the methods.
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References
General
Anderson, H. C. (1978) ‘Probes of membrane structure’ Ann. Rev. Biochem, 47, 359–383.
Nuclear Magnetic Resonance
Bergelson, L. D. (1978) ‘Paramagnetic hydrophilic probes in NMR investigations of membrane systems’ in Methods in Membrane Biology (ed. E. D. Korn), vol. 9, Plenum Press, London, 275–335.
Cullis, P. R. and McLaughlin, A. C. (1977) ‘Phosphorus nuclear magnetic resonance studies of model and biological membranes’ Trends in Biochem. Sci, 196–199.
Lee, A. G. (1978) ‘Fluorescence and NMR studies of membranes’ in Receptors and Recog-nition, vol. AS (ed. P. Cuatrecasas and M. F. Greaves), Chapman and Hall, London,89–131.
Mantsch, H. H., Saito, H. and Smith, I. C. P. (1977) ‘Deuterium magnetic resonance, applications in chemistry, physics and biology’ Prog. NMR Spectroscopy, 11, 211–271.
Seelig, J. (1977) ‘Deuterium magnetic resonance: theory and applications to lipid membranes’ Quart. Rev. Biophys, 10, 353–418.
Seeling, J. (1978) ‘31P Nuclear magnetic resonance and the head group structure of phospholipids in membranes’ Biochim. Biophys. Acta, 515, 105–140.
Electron spin resonance
Berliner, L. J. (ed.) (1976) Spin Labelling, Theory and Applications, Academic Press, New York.
Gaffney, B. J. and Chen, S-C. (1977) ‘Spin-label studies of membranes’ in Methods in
Membrane Biology, vol. 8 (ed. E. D. Korn), Plenum Press, London, 291–358.
Schreier, S., Polnaszek, C. F. and Smith, I. C. P. (1978) ‘Spin labels in membranes.Problems in practice’ Biochim. Biophys. Acta, 515, 375–436.
Fluorescence
Lee, A. G. (1978) ‘Fluorescence and NMR studies of membranes’ in Receptors and Recognition, vol. A5 (ed. P. Cuatrecasas and M. F. Greaves ), Chapman and Hall, London, 81–131.
Radda, G. K. (1975) ‘Fluorescent probes in membrane studies’ in Methods in Membrane Biology, vol. 4 (ed. E. D. Korn ), Plenum Press, New York, 97–188.
Shinitzsky, M. and Barenholz, Y. (1978) ‘Fluidity parameters of lipid regions determined by fluorescence polarization’ Biochim. Biophys. Acta, 515, 367–394.
Diffraction methods
Carey, P. R. (1978) ‘Resonance Raman spectroscopy in biochemistry and biology’ Quart. Rev. Biophys, 11, 309–370.
Henderson, R. and Unwin, P. N. T. (1975) ‘Three dimensional model of purple membrane obtained by electron microscopy’ Nature, 257, 28–32.
Herbette, L., Marguadt, J., Scarpa, A. and Blasie, J. K. (1977) ‘A direct analysis of lamellar X-ray diffraction from hydrated orientated multilayers of fully functional sarcoplasmic reticulum’ Biophys. J, 20, 245–272.
Hui, S. W. (1977) ‘Electron diffraction studies of membranes’ Biochim. Biophys. Acta, 472, 345–371.
Pape, E. H., Klott, K. and Kreutz, W. (1977) ‘The determination of the electron density profile of the human erythrocyte membrane by small-angle X-ray diffraction’ Biophys. J, 19, 141–160.
Schoenborn, B. P. (1976) ‘Neutron scattering for the analysis of membranes’ Biochim. Biophys. Acta, 457, 41–55.
Worthington, C. R. (1976) ‘X-ray studies on membranes’ in The Enzymes of Biological Membranes (ed. A. Martonosi ), Plenum Press, 1–29.
Calorimetry
Mabrey, S. and Sturtevant, J. M. (1978) ‘High sensitivity differential scanning calorimetry in the study of biomembranes and related model systems’ in Methods in Membrane Biology, vol. 9 (ed. E. D. Korn ), Plenum Press, New York, 237–274.
Melchior, D. L. and Steim, J. M. (1976) ‘Thermotropic transitions in biomembranes’ Ann. Rev. Biophys. Bioeng, 5, 205–238.
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© 1980 R. Harrison, G. G. Lunt
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Harrison, R., Lunt, G.G. (1980). Physical Methods Used in the Study of Membranes. In: Biological Membranes. Tertiary Level Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-6857-1_10
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DOI: https://doi.org/10.1007/978-94-011-6857-1_10
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-216-90998-4
Online ISBN: 978-94-011-6857-1
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