Abstract
In 1972, in search of the biological receptor molecule of the phallotoxins, microfilamentous structures were detected in liver membrane preparations of poisoned rats. These microfilaments were scattered over or loosely attached to the plasma membrane fragments1. The chemical nature of the microfilaments was suspected to be actin; the filaments, however, could not be dissolved in 0.6 M KI, conditions which normally depolymerise filamentous actin and hence are a reliable test for this protein. It took more than a year for the microfilaments to be identified as actin by other methods, and it was then realised that the experiment with KI had failed because the toxic action of phalloidin causes irreversible polymerisation of actin.
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References
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© 1975 D. Keppler
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Wieland, T., Schäfer, A., Govindan, V.M., Faulstich, H. (1975). Interaction of phalloidin with actin. In: Keppler, D. (eds) Pathogenesis and Mechanisms of Liver Cell Necrosis. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-6618-8_17
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DOI: https://doi.org/10.1007/978-94-011-6618-8_17
Publisher Name: Springer, Dordrecht
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