Abstract
Developments in laboratory medicine and technology in recent years have greatly increased the demands for high quality animal antisera. The widespread use of antisera in immunochemical procedures and other techniques results from several unique properties possessed by these molecules. Firstly, they have the ability to detect single substances, ranging from high molecular weight proteins to low molecular weight drugs, in complex mixtures of closely related substances. Secondly, reactions involving antibodies are quantitative in nature and thirdly, these reactions may be made extremely sensitive and precise by the application of the appropriate methodology. However, the specificity, sensitivity and precision of immunochemical assays depend to a large extent on the intrinsic characteristics of the antibodies used.
Keywords
- High Molecular Weight Protein
- Radial Immunodiffusion
- Immunochemical Technique
- Antiserum Production
- Laser Nephelometry
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References
MRC Working Party (1971). Recommendations made on the clinical use of immunological reagents. Immunology, 20, 3
Fergusson, M., Hunter, I., Munro, A. C. and Watt, A. (1977). Problems associated with the production of anti-immunoglobulin sera. Presented at Scotblood 77, February 26, Edinburgh
Powell, L. W., Alpert, E., Isselbacher, K. J. and Drysdale, J. W. (1975). Human isoferritins: organ specific iron and apoferritin distribution. Br. J. Haematol., 30, 47
Hazard, J. T., Yokota, M., Arosio, P. and Drysdale, J. W. (1977). Immunologic differences in human isoferritins: complications for immunologic quantitation of serum ferritin. Blood, 49, 139
Van der Giessen, M., de Lange, B. and Van der Lee, B. (1974). The production of precipitating antiglobulin reagents specific for the sub-classes of human IgG. Immunology, 27, 655
Beck, M. L. and Marsh, W. L. (1977). Complement and the antiglobulin. Transfusion, 17, 529
Reimer, C. B., Phillips, D. J., Maddison, S. E. and Shore, S. L. (1970). Comparative evaluation of commercial precipitating antisera against human IgM and IgG. J. Lab. Clin. Med., 76, 949
Phillips, D. J., Shore, S. L., Maddison, S. E., Gordon, D. S. and Reimer, C. B. (1971). Comparative evaluation of commercial precipitating antisera against human IgA. J. Lab. Clin. Med., 77, 639
Reimer, C. B. and Maddison, S. E. (1976). Standardization of human immunoglobulin quantitation: a review of current status and problems. Clin. Chem., 22, 577
Ritchie, R. F. (1972). Evaluation of antibody preparations for the AIP system. Presented at the Colloquium on AIP, May 2, Brussels
Moore, J. A. and Chaplin, H. Jr. (1974). Anti-C3d antiglobulin reagents I. Characteristics of the anti-C3c and anti-C3d responses during hyperimmunization in rabbits. Transfusion, 14, 407
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© 1979 MTP Press Limited
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Munro, A.C. (1979). Antiserum requirements. In: Ward, A.M., Whicher, J.T. (eds) Immunochemistry in Clinical Laboratory Medicine. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-6591-4_4
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DOI: https://doi.org/10.1007/978-94-011-6591-4_4
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-011-6593-8
Online ISBN: 978-94-011-6591-4
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