Renal function impairment in swelling with and without anoxia
As improvement in organ preservation has been obtained with hyperosmolar solutions, it has been deduced that inhibition of cellular swelling was beneficial, and that cellular swelling was one of the major pathways leading to cellular death. Disruption of cellular architecture and spatial separation of key enzymes are the suspected mechanisms. If this is the case, swelling in absence of anoxia should produce the same defects as anoxia. We have therefore attempted to produce similar swelling by flushing kidneys with hypotonic solutions or by submitting them to normothermic anoxia. The effects on glomerular function tested immediately or after 24 h were markedly different according to the origin of the oedema.
KeywordsRenal Blood Flow Organ Preservation Hypotonic Solution Cellular Death Isotonic Solution
Unable to display preview. Download preview PDF.
- 2.Krebs, H. (1971). Report of a meeting: workshop on organ preservation. Surgery, 69, 321Google Scholar
- 9.Grundmann, R., Eichmann, J., Keckstein, J., Raab, M., Meusel, E. and Pichlmaier, H. (1977). Relationship between the prolongation of warm ischemia and the maximum available preservation period. Surgery, 81, 512Google Scholar
- 10.Grundmann, R., Bischoff, A., Albrod, A. and Pichlmaier, H. (1979). Canine kidney perfusion after various warm ischaemic periods. In Pegg, D. E. and Jacobsen, I. A. (eds.) Organ Preservation II, pp. 33–45. ( Edinburgh: Churchill Livingstone )Google Scholar
- 13.Green, C.J. and Pegg, D.E. (1979). The effect of variation in electrolyte composition and osmolality of solutions for infusion and hypothermic storage of kidneys. In Pegg, D.E. and Jacobsen, I.A. (eds.) Organ Preservation II, pp. 86–100. ( Edinburgh: Churchill Livingstone )Google Scholar
- 14.Lambotte, L. and Wojcik, S. (1979). Ouabain inhibits the recovery of cellular volume in liver perfused with hypo-osmolar solution. Arch. Int. Phys. Bioch., 87, 335Google Scholar
- 16.Fischer, J. H., Miyata, M., Isselhard, W. and Casser, H. R. (1979). Hypotherme lagerung unter aeroben bedingungen. Einfluss unterschiedlicher freispiillosungen auf die function- serhaltung der niere. Langenbecks Arch. Chir., suppl., 307Google Scholar