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Endothelial cell function and organ preservation ex vivo

  • J. D. Pearson
  • A. Hutchings
  • J. L. Gordon

Abstract

The endothelial cell monolayer that lines blood vessels forms the interface between circulating blood and extravascular tissue, and it controls the egress of soluble blood components and formed elements of the blood. Excessive leakage of small molecules or of blood cells is prevented, whilst the transport of appropriate nutrients is permitted, as is the emigration of specific classes of white cell from the vessel lumen under certain circumstances.

Keywords

Endothelial Cell Smooth Muscle Cell Plasminogen Activator Factor VIII Human Endothelial Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Pegg, D.E. (1971). Vascular resistance of the isolated rabbit kidney. Cryobiology, 8, 431PubMedCrossRefGoogle Scholar
  2. 2.
    Belzer, F.O., Hoffman, R., Huang, J. and Downes, G. (1972). Endothelial damage in perfused dog kidney and cold sensitivity of vascular Na-K-ATPase. Cryobiology, 9, 457PubMedCrossRefGoogle Scholar
  3. 3.
    Pegg, D.E. and Green, C.J. (1973). The functional state of kidneys perfused at 37°C with a bloodless fluid. J. Surg. Res., 15, 218PubMedCrossRefGoogle Scholar
  4. 4.
    Dvorak, H.F., Mihm, M.C., Dvorak, A., Barnes, B. A., Manseau, E.J. and Galli, S.J. (1979). Rejection of first-set skin allografts in man. The microvasculature is the critical target of the immune response. J. Exp. Med., 150, 322PubMedCrossRefGoogle Scholar
  5. 5.
    Paul, L. C., Van Es, L. A., Van Rood, J. J., Van Leeuwen, A., de la Riviere, G. B. and de Graeff, J. (1979). Antibodies directed against antigens on the endothelium of peritubular capillaries in patients with rejecting renal allografts. Transplantation, 27, 175Google Scholar
  6. 6.
    Thorgeirsson, G. and Robertson, A. L. (1978). The vascular endothelium - pathobiologic significance. Am. J. Pathol., 93, 803PubMedGoogle Scholar
  7. 7.
    Gimbrone, M. A. (1976). Culture of vascular endothelium. In Spaet, T. H. (ed.), Progress in Hemostasis and Thrombosis. Vol. III, pp. 1–28. ( New York: Grune & Stratton )Google Scholar
  8. 8.
    Weksler, B.B. (1982). Prostaglandins and the endothelial cell. In Herman, A.G., Vanhoutte, P. M., Denolin, H. and Goossens, A. (eds.) Cardiovascular Pharmacology of the Prostaglandins. ( New York: Raven Press) (In press )Google Scholar
  9. 9.
    Jaffe, E.A., Hoyer, L.W. and Nachman, R.L. (1973). Synthesis of antihemophilic factor antigen by cultured human endothelial cells. J. Clin. Invest., 52, 2757PubMedCrossRefGoogle Scholar
  10. 10.
    Ryan, U. S., Ryan, J. W., Whitaker, C. and Chiu, A. (1976). Localization of angiotensin converting enzyme II immunocytochemistry and immunofluorescence. Tissue Cell, 8, 125PubMedCrossRefGoogle Scholar
  11. 11.
    Bakhle, Y.S. and Vane, J.R. (1974). Pharmacokinetic functions of the pulmonary circulation. Pharmacol. Rev., 54, 1007Google Scholar
  12. 12.
    Gillis, C.N. and Roth, J.A. (1976). Pulmonary disposition of circulating vasoactive hormones. Biochem. Pharmacol., 25, 2547PubMedCrossRefGoogle Scholar
  13. 13.
    Trevethick, M.A., Olverman, H.J., Pearson, J.D., Gordon, J.L., Lyles, G.A. and Callingham, B. A. (1981). Monoamine oxidase activities of porcine vascular endothelial and smooth muscle cells. Biochem. Pharmacol., 30, 2209PubMedCrossRefGoogle Scholar
  14. 14.
    Pearson, J.D., Carleton, J.S., Hutchings, A. and Gordon, J.L. (1978). Uptake and metabolism of adenosine by pig aortic endothelial and smooth muscle cells in culture. Biochem. J., 170, 265PubMedGoogle Scholar
  15. 15.
    Pearson, J.D., Carleton, J.S. and Gordon, J.L. (1980). Metabolism of adenine nucleotides by ectoenzymes of vascular endothelial and smooth muscle cells in culture. Biochem. J., 190, 421PubMedGoogle Scholar
  16. 16.
    Moncada, S., Gryglewski, R., Bunting, S. and Vane, J.R. (1976). An enzyme isolated from arteries transforms prostaglandin endoperoxides to an unstable substance that inhibits platelet aggregation. Nature (Lond.), 263, 663CrossRefGoogle Scholar
  17. 17.
    Weksler, B.B., Marcus, A.J. and Jaffe, E. A. (1977). Synthesis of prostaglandin I2 by cultured human and bovine endothelial cells. Proc. Natl. Acad. Sci., (USA), 74, 3922CrossRefGoogle Scholar
  18. 18.
    Maclntyre, D.E., Pearson, J.D. and Gordon, J.L. (1978). Localization and stimulation of prostacyclin production in vascular cells. Nature (Lond.), 271, 549CrossRefGoogle Scholar
  19. 19.
    Best, L.C., Martin, T.J., Russell, R.G.G. and Preston, F.E. (1977). Prostacyclin increases cyclic AMP levels and adenylate cyclase activity in platelets. Nature (Lond.), 267, 850CrossRefGoogle Scholar
  20. 20.
    Czervionke, R.L., Smith, J.B., Fry, G.L., Hoak, J.C. and Haycraft, D.L. (1979). Inhibition of prostacyclin by treatment of endothelium with aspirin. Correlation with platelet adherence. J. Clin. Invest., 63, 1089PubMedCrossRefGoogle Scholar
  21. 21.
    Curwen, K.D., Gimbrone, M.A. and Handin, R.I. (1980). In vitro studies of thrombo- resistance. The role of prostacyclin in platelet adhesion to cultured normal and virally transformed human vascular endothelial cells. Lab. Invest., 42, 366Google Scholar
  22. 22.
    Higgs, E. A., Moncada, S., Vane, J. R., Caen, J. P., Michel, M. and Tobelem, G. (1978). Effect of prostacyclin on platelet adhesion to rabbit arterial subendothelium. Prostaglandins, 16, 17PubMedCrossRefGoogle Scholar
  23. 23.
    Weksler, B.B., Ley, C.W. and Jaffe, E. A. (1978). Stimulation of endothelial prostacyclin production by thrombin, trypsin, and the ionophore A23187. J. Clin. Invest., 62, 923PubMedCrossRefGoogle Scholar
  24. 24.
    Hong, S. L. (1980). Effect of bradykinin and thrombin on prostacyclin synthesis in endothelial cells from calf and pig aorta and human umbilical vein. Thromb. Res., 18, 787PubMedCrossRefGoogle Scholar
  25. 25.
    Needleman, P., Wyche, A. and Raz, A. (1979). Platelet and blood vessel arachidonate metabolism and interactions. J. Clin. Invest., 63, 345PubMedCrossRefGoogle Scholar
  26. 26.
    Marcus, A.J., Weksler, B.B., Jaffe, E.A. and Broekman, M.J. (1980). Synthesis of prostacyclin from platelet-derived endoperoxides by cultured human endothelial cells. J. Clin. Invest., 66, 979PubMedCrossRefGoogle Scholar
  27. 27.
    Coughlin, S.R., Moskowitz, M.A., Zetter, B.R., Antoniades, H.N. and Levine, L. (1980). Platelet-dependent stimulation of prostacyclin synthesis by platelet-derived growth factor. Nature (Lond.), 288, 600CrossRefGoogle Scholar
  28. 28.
    Lollar, P. and Owen, W. G. (1980). Clearance of thrombin from circulation in rabbits by high-affinity binding sites on endothelium. J. Clin. Invest., 66, 1222PubMedCrossRefGoogle Scholar
  29. 29.
    Awbrey, B.J., Hoak, J.C. and Owen, W.G. (1979). Binding of human thrombin to cultured endothelial cells. J. Biol. Chem., 254, 4092PubMedGoogle Scholar
  30. 30.
    Pearson, J. D. and Gordon, J. L. (1979). Vascular endothelial and smooth muscle cells in culture selectively release adenine nucleotides. Nature (Lond.), 281, 384CrossRefGoogle Scholar
  31. 31.
    Lollar, P. and Owen, W.G. (1980). Evidence that the effects of thrombin on arachidonate metabolism in cultured human endothelial cells are not mediated by a high affinity receptor. J. Biochem., 255, 8031Google Scholar
  32. 32.
    Lollar, P. and Owen, W.G. (1981). Active site dependent, thrombin-induced release of adenine nucelotides from cultured human endothelial cells. Ann. N. Y. Acad. Sci., 307, 51CrossRefGoogle Scholar
  33. 33.
    Chan, V. and Chan, K. (1979). Anti-thrombin III in fresh and cultured human endothelial cells: a natural anticoagulant from the vascular endothelium. Thromb. Res., 15, 209PubMedCrossRefGoogle Scholar
  34. 34.
    Kowalski, S. and Finlay, T. H. (1979). Heparin and the inactivation of thrombin by anti-thrombin III. Thromb. Res., 14, 387PubMedCrossRefGoogle Scholar
  35. 35.
    Buonassisi, V. (1973). Sulfated mucopolysaccharide synthesis and secretion in endothelial cell cultures. Exp. Cell Res., 76, 363PubMedCrossRefGoogle Scholar
  36. 36.
    Busch, C., Ljungman, C., Holdin, C.-M., Waskson, E. and Obrink, B. (1979). Surface properties of cultured endothelial cells. Haemostasis, 8, 142PubMedGoogle Scholar
  37. 37.
    Long, W. F., Williamson, F. B., Kindness, G. and Edward, M. (1980). The anticoagulant activity of dermatan sulphates. Thromb. Res., 18, 493PubMedCrossRefGoogle Scholar
  38. 38.
    Busch, C., Dawes, J., Pepper, D. S. and Wasteson, A. (1980). Binding of platelet factor 4 to cultured human umbilical vein endothelial cells. Thromb. Res., 19, 129PubMedCrossRefGoogle Scholar
  39. 39.
    Todd, A.S. (1959). The histological localisation of fibrinolysin activator. J. Pathol. Bacteriol, 78, 281PubMedCrossRefGoogle Scholar
  40. 40.
    Pugatch, E.M.J, and Poole, J.C.F. (1969). Studies on the fibrinolytic activity of an extract from vascular endothelium. Q. J. Exp. Physiol., 54, 80Google Scholar
  41. 41.
    Izaki, S. and Kitaguchi, H. (1977). Calcium dependent and independent release of plasminogen activator from the vessel wall. Thromb. Res., 10, 765PubMedCrossRefGoogle Scholar
  42. 42.
    Rijken, D.C., Wijngaards, G. and Welbergen, J. (1980). Relationship between tissue plasminogen activator and the activators in blood and vascular wall. Thromb. Res., 18, 815PubMedCrossRefGoogle Scholar
  43. 43.
    Loskutoff, D.J. and Edgington, T.S. (1977). Synthesis of a fibrinolytic activator and inhibitor by endothelial cells. Proc. Natl. Acad. Sci., (USA), 74, 3903CrossRefGoogle Scholar
  44. 44.
    Levin, E.G. and Loskutoff, D.J. (1980). Serum-mediated suppression of cell-associated plasminogen activator activity in cultured endothelial cells. Cell, 22, 701PubMedCrossRefGoogle Scholar
  45. 45.
    Maynard, J.R., Dreyer, B.E., Stemerman, M.B. and Pitlick, F. A. (1977). Tissue factor coagulant activity of cultured human endothelial and smooth muscle cells and fibroblasts. Blood, 50, 387PubMedGoogle Scholar
  46. 46.
    Jaffe, E.A., Hoyer, L.E. and Nachman, R.L. (1974). Synthesis of von Willebrand factor by cultured human endothelial cells. Proc. Natl. Acad. Sci., (USA), 71, 1906CrossRefGoogle Scholar
  47. 47.
    Shearn, S.A.M., Peake, I.R., Giddings, J.C., Humphrys, J. and Bloom, A.L. (1977). The characterisation and synthesis of antigens related to factor VIII in vascular endothelium. Thromb. Res., 11, 43PubMedCrossRefGoogle Scholar
  48. 48.
    Stead, N. W. and McKee, P. A. (1979). The effect of cultured endothelial cells on factor VIII procoagulant activity. Blood, 54, 560PubMedGoogle Scholar
  49. 49.
    Woodruff, J.J. and Kuttner, B.J. (1980). Adherence of lymphocytes to the high endothelium of lymph nodes in vitro. CIBA Found. Symp., 71, 243PubMedGoogle Scholar
  50. 50.
    Graham, R.C. and Shannon, S.L. (1972). Peroxidase arthritis II lymphoid cell-endo- thelial interactions during a developing immunologic inflammatory response. Am. J. Pathol., 69, 7PubMedGoogle Scholar
  51. 51.
    Smith, J.B., Mcintosh, G.M. and Morris, B. (1970). The migration of cells through chronically inflamed tissues. J. Pathol., 100, 21PubMedCrossRefGoogle Scholar
  52. 52.
    Wilkinson, P.C. and Lackie, J.M. (1979). The adhesion, migration and Chemotaxis of leucocytes in inflammation. Curr. Top. Pathol., 68, 47PubMedCrossRefGoogle Scholar
  53. 53.
    MacGregor, R. R. (1980). Granulocyte adherence. In Weissman, G. (ed.), Cell Biology of Inflammation, pp. 267–298. ( Amsterdam: Elsevier )Google Scholar
  54. 54.
    Pearson, J.D. and Gordon, J.L. (1981). Granulocyte interactions with endothelium. In Gordon, J.L. and Dingle, J.T. (eds.) Cellular Interactions, pp. 107–118. ( Amsterdam: Elsevier )Google Scholar
  55. 55.
    Beesley, J.E., Pearson, J.D., Carleton, J.S., Hutchings, A. and Gordon, J.L. (1978). Interactions of leukocytes with vascular cells in culture. J. Cell Sci., 33, 85PubMedGoogle Scholar
  56. 56.
    Lackie, J.M. and Smith, R.P.C. (1980). Interactions of neutrophil granulocytes and endothelium. In Curtis, A.S.G. and Pitts, J.D. (eds.) Cell Adhesion and Motility, pp. 235–272. ( Cambridge: Cambridge University Press )Google Scholar
  57. 57.
    Beesley, J.D., Pearson, J.D., Hutchings, A., Carleton, J.S. and Gordon, J.L. (1979). Granulocyte migration through endothelium in culture. J. Cell Sci., 38, 237PubMedGoogle Scholar
  58. 58.
    Thompson, J.S., Overlin, V., Severson, C.D., Parsons, T.J., Herbick, J., Strauss, R. G., Burns, C. P. and Claas, F. H. J. (1980). Demonstration of granulocyte, monocyte, and endothelial cell antigens by double fluorochromatic microcytotoxicity testing. Transpl. Proc., 12 (suppl. 1), 26Google Scholar
  59. 59.
    Stasny, P. (1980). Endothelial-monocyte antigens. Transpl. Proc., 12 (suppl. 1), 32Google Scholar
  60. 60.
    Cerilli, J. and Brasile, L. (1980). Endothelial cell alloantigens. Transpl. Proc., 12 (suppl. 1), 37Google Scholar
  61. 61.
    Porter, K. A., Calne, R. Y. and Zukoski, C.F. (1964). Vascular and other changes in 200 canine renal homotransplants treated with immunosuppressive drugs. Lab. Invest., 13, 809Google Scholar
  62. 62.
    Hirschberg, H., Moen, T. and Thorsby, E. (1979). Specific destruction of human endothelial cell monolayers by anti-DRW antisera. Transplantation, 28, 116PubMedCrossRefGoogle Scholar
  63. 63.
    Burger, D.R., Ford, D., Hamblin, A. and Dumonde, D. (1981). Endothelial cell presentation of antigen to human T cells. Hum. Immunol., 2 (In press)Google Scholar
  64. 64.
    De Bono, D. (1976). Endothelium-lymphocyte interactions in vitro. I. Adherence of non-allergised lymphocytes. Cell Immunol., 26, 78PubMedCrossRefGoogle Scholar
  65. 65.
    De Bono, D. (1979). Endothelium-lymphocyte interactions in vitro. II. Adherence of allergised lymphocytes. Cell Immunol., 44, 64PubMedCrossRefGoogle Scholar

Copyright information

© MTP Press Limited 1982

Authors and Affiliations

  • J. D. Pearson
  • A. Hutchings
  • J. L. Gordon

There are no affiliations available

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