Renal viability testing during preservation by metabolic parameters
Many attempts have been made to find a method for renal viability testing during the preservation period. Most of the techniques have turned out to be of rather limited value. Because of the central importance of the energy metabolism for the survival of cells in unphysiological situations, the substances of the energy-distributing system (in the kidney, mainly the adenine nucleotides) as well as the metabolites of the glycolytic pathway (the only way for energy production in anaerobiosis) should be excellent parameters for viability1–4. Our experience includes experiments on several species (dogs, rats and guinea pigs) as well as different preservation techniques (normothermic ischaemia, hypothermic ischaemia, continuous hypothermic perfusion or retrograde oxygen persufflation (ROP)5–8).
KeywordsAdenine Nucleotide Organ Preservation Continuous Perfusion Preservation Period Normothermic Ischaemia
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