Abstract
The barley stem rust resistance genes Rpg1 and rpg4 were mapped in barley on chromosomes 1P and 7M, respectively and the syntenous rice chromosomes identified as 6P and 3P by mapping common probes in barley and rice. Rice yeast artificial chromosome (YAC), bacterial artificial chromosome (BAC) and cosmid clones were used to isolate probes mapping to the barley Rpg1 region. The rice BAC isolated with the pM13 probe was a particularly excellent source of probes. A high-resolution map of the Rpg1 region was established with 1400 gametes yielding a map density of 3.6 markers per 0.1 cM. A detailed physical map was established for the rice BAC fragment containing the Rpg1-flanking markers pM13 and B24. This fragment covers a barley genetic distance of 0.6 cM and a rice DNA physical distance of ca. 70 kb. The distribution of barley cross-overs in relation to the rice DNA physical distances was extremely uneven. The barley genetic distance between the pM13 marker and Rpg1 was 0.1 cM per ca. 55 kb, while on the proximal side it was 0.5 cm per ca. 15 kb. Three probes from the distal end of the pM 13 BAC mapped 3.0 cm proximal of Rpg1 and out of synteny with rice. These experiments confirm the validity of using large insert rice clones as probe sources to saturate small barley (and other large genome cereals) genome regions with markers. They also establish a note of caution that even in regions of high microsynteny, there may be small DNA fragments that have transposed and are no longer in syntenous positions.
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References
Ahn S, Tanksley SD: Comparative linkage maps of the rice and maize genomes. Proc Natl Acad Sci USA 90: 7980–7984 (1993).
Arumuganathan K, Earle ED: Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9: 208–218 (1991).
Ausubel FM, Brent R, Kingston RE, Moore DM, Seidman JG, Smith JA, Strahl K: Current Protocols in Molecular Biology. John Wiley, New York (1987).
Bennetzen JL, Freeling M: Grasses as a single genetic system: genome composition, collinearity and compatibility. Trends Genet 9: 259 (1993).
Bent AF, Kunkel BN, Dahlbeck D, Brown KL, Schmidt R, Giraudat J, Leung J, Staskawicz BJ: RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes. Science 265: 1856–1860 (1994).
Borovkova IG, Steffenson BJ, Jin Y, Rasmussen JB, Kilian A, Kleinhofs A, Rossnagel BG, Kao KN: Identification of molecular markers linked to the stem rust resistance gene rpg4 in barley. Phytopathology 85: 181–185 (1995).
Devos KM, Millan T, Gale MD: Comparative RFLP maps of homoeologous chromosomes of wheat, rye and barley. Theor Appl Genet 85: 784–792 (1993).
Dubcovsky J, Luo M-C, Zhong G-Y, Kilian A, Kleinhofs A, Dvorak J: Genetic map of diploid wheat, Triticum monococcum L., and its comparison with maps of Hordeum vulgare L. Genetics, in press (1996).
Dunford RP, Kurata N, Laurie DA, Money TA, Minobe Y, Moore G: Conservation of fine-scale DNA marker order in the genomes of rice and the Triticeae. Nucl Acids Res 23: 2724–2728 (1995).
Flor HH: Inheritance of reaction to rust in flax. J Agric Res 74: 241–262 (1947).
Harlan JR: Crops & Man. American Society of Agronomy; Crop Science Society of America, Madison, WI (1992).
Jin Y, Steffenson BJ, Fetch TG: Sources of resistance to pathotype QCC of Puccinia graminis f. sp. tritici in barley. Crop Sci 34: 285–288 (1994).
Jin Y, Steffenson BJ, Miller JD: Inheritance of resistance to pathotypes QCC and MCC of Puccinia graminis f. sp. tritici in barley line Q21861 and temperature effects on the expression of resistance. Phytopathology 84: 452–455 (1994).
Johal GS, Briggs SP: Reductase activity encoded by the HMI disease resistance gene in maize. Science 258: 985–987 (1992).
Jones DA, Thomas CM, Hammond-Kosack KE, Balint-Kurti PJ, Jones JDG: Isolation of the tomato Cf-9 gene for resistance to Cladosporium fulvum by transposon tagging. Science 266: 789–793 (1994).
Kilian A, Kudrna DA, Kleinhofs A, Yano M, Kurata N, Steffenson B, Sasaki T: Rice-barley synteny and its application to saturation mapping of the barley Rpgl region. Nucl Acids Res 23: 2729–2733 (1995).
Kilian A, Steffenson BJ, Maroof MAS, Kleinhofs A: RFLP markers linked to the durable stem rust resistance gene Rpgl in barley. Mol Plant-Microbe Interact 7: 298–301 (1994).
Kleinhofs A, Kilian A, Maroof MAS, Biyashev RM, Hayes P, Chen FQ, Lapitan N, Fenwick A, Blake TK, Kanazin V, Ananiev E, Dahleen L, Kudrna D, Bollinger J, Knapp SJ, Liu B, Sorrells M, Heun M, Franckowiak JD, Hoffman D, Skadsen R, Steffenson BJ: A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theor Appl Genet 86: 705–712 (1993).
Kurata N, Moore G, Nagamura Y, Foote T, Yano M, Minobe Y, Gale M: Conservation of genome structure between rice and wheat. Bio/technology 12: 276–278 (1994).
Kurata N, Nagamura Y, Yamamoto K, Harushima Y, Sue N, Wu J, Antonio BA, Shomura A, Shimizu T, Lin S-Y, Inoue T, Fukuda A, Shimano T, Kuboki Y, Toyama T, Miyamoto Y, Kirihara T, Hayasaka K, Miyao A, Monna L, Zhong HS, Tamura Y, Wang Z-X, Momma T, Umehara Y, Yano M, Sasaki T, Minobe Y: A 300 kilobase interval genetic map of rice including 883 expressed sequences. Nature Genet 8: 365–372 (1994).
Lamb CJ: Plant disease resistance genes in signal perception and transduction. Cell 76: 419–422 (1994).
Lawrence GJ, Finnegan EJ, Ayliffe MA, Ellis JG: The L6 gene for flax rust resistance is related to the Arabidopsis bacterial resistance gene RPS2 and the tobacco viral resistance gene N. Plant Cell 7: 1195–1206 (1995).
Martin GM, Brommonschenkel SH, Chunwongse J, Frary A, Ganal MW, Spivey R, Wu T, Earle ED, Tanksley SD: Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science 262: 1432–1436 (1993).
Melake-Berhan A, Hulbert SH, Butler LG, Bennetzen JL: Structure and evolution of the genomes of Sorghum bicolor and Zea mays. Theor Appl Genet 86: 598 (1993).
Mindrinos M, Katagiri F, Yu G-L, Ausubel FM: The A. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats. Cell 78: 1089–1099 (1994).
Moore G, Abbo S, Cheung W, Foote T, Gale MD, Koebner R, Leitch A, Leitch I, Money T, Stancombe P, Yano M, Flay-ell R: Key features of cereal genome organization as revealed by the use of cytosine-methylation sensitive restriction endonucleases. Genomics 15: 472–482 (1993).
Roelfs AP, Casper DH, Long DL, Roberts JJ: Races of Puccinia graminis in the United States in 1989. Plant Dis 75: 1127–1130 (1991).
Roelfs AP, Long DL, Roberts JJ: Races of Puccinia graminis in the United States during 1991. Plant Dis 77: 129–132 (1993).
Siedler H, Graner A: Construction of physical maps of the Horl locus of two barley cultivars by pulsed field gel electrophoresis. Mol Gen Genet 226: 177–181 (1991).
Song W-Y, Wang G-L, Chen L-L, Kim H-S, Pi L-Y, Gardner J, Holsten T, Wang B, Zhai W-X, Zhu L-H, Fauquet C, Ronald P: A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Science 270: 1804–1806 (1995).
Sorensen MB: Mapping of the Hor2 locus in barley by pulsed field gel electrophoresis. Carlsberg Res Commun 54: 109–120 (1989).
Steffenson BJ: Analysis of durable resistance to stem rust in barley. Euphytica 63: 153–167 (1992).
Sun Y, Steffenson BJ, Jin Y: Genetics of resistance to Puccinia graminis f. sp. secalis in barley line Q21861. Phytopathology 86, in press (1996).
Traut TW: The functions and consensus motifs of nine types of peptide segments that form different types of nucleotide-binding sites. Eur J Biochem 222: 9–19 (1994).
Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Homes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M: AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 23: 4407–4414 (1995).
Whitkus R, Doebley J, Lee M: Comparative genome mapping of sorghum and maize. Genetics 132: 1119–1130 (1992).
Whitman S, Dinesh-Kumar SP, Choi D. Hehl R, Corr C, Baker B: The product of the tobacco mosaic virus resistance gene N: similarity to toll and the interleukin-1 receptor. Cell 78: 1101–1115 (1994).
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Kilian, A., Chen, J., Han, F., Steffenson, B., Kleinhofs, A. (1997). Towards map-based cloning of the barley stem rust resistance genes Rpg1 and Rpg4 using rice as an intergenomic cloning vehicle. In: Sasaki, T., Moore, G. (eds) Oryza: From Molecule to Plant. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5794-0_18
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DOI: https://doi.org/10.1007/978-94-011-5794-0_18
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