Abstract
The aim of the present work was to investigate the sequence of some cellular and metabolic events occurring in the embryonic axes, which might be related to the loss of viability of recalcitrant Araucaria angustifolia embryos during dehydration in the open air at 25°C and 55% relative humidity. The decreases in the ability for protein synthesis and in the capacity to convert 1-aminocyclopropane 1-carboxylic acid to ethylene, which were observed respectively at 0.5 and 1.5 h of dehydration, were very early indicators of deterioration. A high increase in leakage of electrolytes, which indicated a deterioration of cell membrane properties, was observed by the third–fourth h of desiccation. ATP content and energy charge also rapidly decreased during dehydration. However, energy charge cannot be a good marker of damage, since reimbibition of embryos restored its value close to that measured in non-dehydrated axes.
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References
Atkinson, D.E. 1968. Biochemistry 7: 4030–4034.
Becwar, M.R., Stanwood, P.C. and Roos, E.E. 1982. Plant Physiology 69: 1132–1135.
Bewley, J.D. 1979. Annual Review of Plant Physiology 30: 195–238.
Bewley, J.D. and Black, M. 1994. Seeds. Physiology of Development and Germination, Second Edition, pp. 445. New York, London: Plenum Press.
Ghin, H.F. and Roberts, E.H. 1980. Recalcitrant Crop Seeds, pp. 152. Kuala Lumpur: Tropical Press SDN.
Corbineau, F. and Côme, D. 1986. Seed Science and Technology 14: 585–591.
Corbineau, F. and Côme, D. 1988. Seed Science and Technology 16: 97–103.
Fu, J.R., Zhang, B.Z., Wang, X.F., Qiao, Y.Z. and Huang, X.L. 1990. Seed Science and Technology 18: 743–754.
Hendry, G.A.F., Finch-Savage, W.E., Thorpe, P.C., Atherton, N.M., Buckland, S., Nilson, K.A. and Seel, W.E. 1992. New Phytologist 122: 273–279.
King, M.W and Roberts, E.H. 1979. The storage of recalcitrant seeds. Achievements and possible approaches, pp. 96. Rome: International Board for Plant Genetic Resources.
Leprince, O., Deltour, R., Thorpe, P.C., Atherton, N.M. and Hendry, G.A.F. 1990. New Phytologist 116: 573–580.
Odawara, S.A., Watanabe, H. and Imaseki, H. 1977. Plant Physiology 18: 569–575.
Olempska-Beer, Z. and Bautz Freeze, E. 1984. Analytical Biochemistry 140: 236–245.
Pammenter, N.W., Vertucci, C. and Berjak, P. 1991. Plant Physiology 96: 1093–1098.
Poulsen, K.M. and Eriksen, E.N. 1992. Seed Science Research 2: 215–221.
Priestley, D.A. 1986. Seed aging. Implications for seed storage and persistance in the soil, pp. 304. Ithaca, New York: Cornell University Press.
Roberts, E.H. 1973. Seed Science and Technology 1: 499–514.
Saglio, P.H., Daniels, M. J. and Pradet, A. 1979. Journal of General Microbiology 110: 13–20.
Salmen Espindola, L., Noin, M., Corbineau, F. and Côme, D. 1994. Seed Science Research 4: 193–201.
Strehler, B.L. and Totter, J.R. 1952. Archives of Biochemistry and Biophysics 40: 28–40.
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Corbineau, F., Espindola, L.S., Vinel, D., Côme, D. (1997). Cellular and Metabolic Events Associated with Dehydration of Recalcitrant Araucaria angustifolia Embryos. In: Ellis, R.H., Black, M., Murdoch, A.J., Hong, T.D. (eds) Basic and Applied Aspects of Seed Biology. Current Plant Science and Biotechnology in Agriculture, vol 30. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5716-2_78
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DOI: https://doi.org/10.1007/978-94-011-5716-2_78
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