Abstract
Using flow cytometry, we observed that priming treatments in PEG solutions might induce DNA replication in the embryo root tips of pepper seeds. Under the same osmotic condition the amount of induced DNA synthesis was proportional to the length of the treatment and its effectiveness in improving seed performance, as measured by the reduction in mean germination time (MGT). However, different osmotic treatments might exert the same effect on MGT while inducing a different amount of nuclei to enter the synthetic phase. The activation and progress in DNA synthesis during priming, therefore, appear to be strongly influenced by the osmotic conditions and length of the treatment.
Osmoconditioning of controlled deteriorated seeds exerted various effects on seed germination, depending on seed deterioration. Under the same treatment, the amount of priming-induced DNA synthesis was lower than in unaged seeds or it was not induced at all.
Osmotic treatments considerably lowered seed tolerance to adverse storage conditions as compared with untreated seeds. However, seeds in which DNA replication was induced by priming were more sensitive to controlled deterioration than seeds in which priming did not induce nuclei to enter the synthetic phase.
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References
Argerich, CA., Bradford, K.J. and Tarquis, A.M. 1989. Journal of Experimental Botany 40: 593–598.
Ashraf, M. and Bray, CM. 1993. Seed Science Research 3: 15–23.
Bradford, K.J. 1990. Plant Physiology 94: 840–849.
Bray, CM., Davison, P.A., Ashraf, M. and Taylor, R.M. 1989. Annuls of Botany 63:185–193.
Coolbear, P., Slater, R.J. and Bryant, J. A. 1990. Annals of Botany 65: 187–195.
Cruz García, F., Jimenez, L.F. and Vázquez-Ramos, J.M. 1995. Seed Science Research 5: 15–33.
Davison, P.A. and Bray, CM. 1991. Seed Science Research 1: 29–35.
Dell’Aquila, A., Savino, G. and De Leo, P. 1978. Plant and Cell Physiology 19: 348-354.
Fujikura, Y. and Karssen, C 1992. Seed Science Research 2: 23–31.
Georghiou, K., Thanos, CA. and Passam, H.C 1987. Annals of Botany 60: 279–285.
ISTA. 1993. Seed Science and Technology 21, Supplement, Rules 1993.
Koopman, M.J.F. 1963. Proceedings of the International Seed Testing Associations 28: 853–860.
Lanteri, S., Kraak, L., De Vos, C.H.R. and Bino, R.J. 1993. Physiologia Plantarum 89: 433–440.
Osborne, D.J. 1983. Canadian Journal of Botany 61: 3568–3577.
Pandey, D.K. 1989. Seed Science and Technology 17: 391–397.
Roberts, E.H. 1981. Seed Science and Technology 9: 359–372.
Saxena, P.K. and King, J. 1988. In: Biotechnology in Agricultural and Forestry. Plant Protoplast and Genetic Engineering, pp 328–342 (ed. Y.P.S Bajaj). New York: Springer-Verlag.
Tarquis, A.M. and Bradford, K.J. 1992. Journal of Experimental Botany 43: 307–317.
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Lanteri, S., Belletti, P., Marzach, C., Nada, E., Quagliotti, L., Bino, R.J. (1997). Priming-induced Nuclear Replication Activity in Pepper (Capsicum annuum L.) Seeds. Effect on Germination and Storability. In: Ellis, R.H., Black, M., Murdoch, A.J., Hong, T.D. (eds) Basic and Applied Aspects of Seed Biology. Current Plant Science and Biotechnology in Agriculture, vol 30. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5716-2_49
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DOI: https://doi.org/10.1007/978-94-011-5716-2_49
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