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Measurement of cytokine antibodies. Test development

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Antibodies in Cytokines

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Abstract

Several assays have been used for detection of antibodies against cytokines. The choice of assay is greatly dependent on the intended goal, e.g. detection of naturally occurring antibodies or therapy induced antibodies. The different assays can be grouped in 2 categories. The interference or indirect assays are based on the detection of the test sample interference with the biological activity, with detection of the cytokine in EIA or with binding to cellular receptors. In direct assays cytokine antibodies are detected by binding to solid phase fixed cytokines, followed by incubation with a secondary enzyme-labelled anti-human Ig antibody or by binding to 125I-labelled cytokines in RIA.

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Abbreviations

Ab-IFNα:

antibodies to alpha interferon

Ab-IFNβ :

antibodies to beta interferon

Ala:

alanine

Arg:

arginine

Asp:

aspartic acid

Bo:

bovine

Cys:

Cysteine

Gln:

glutamine

Glu:

Glutamic Acid

Gly:

glycine

His:

histidine

Hu:

human

IFN:

interferon

IFNα:

alpha interferon

IFNβ :

beta interferon

IFNγ:

gamma interferon

IFNω:

omega interferon

IFNÏ„:

tau interferon

IFNA:

gene of alpha interferon

IFNB:

gene of beta interferon

IFNW:

gene og omega interferon

Ile:

isoleucine

Jak:

Janus kinase

LE-IFNα:

leukocyte alpha interferon

Leu:

leucine

LY-IFNω:

lymphoblastoid alpha interferon

Lys:

Lysine

mAb:

monoclonal antibody

Met:

methionine

Mu:

Murine

N-Ab-IFNα:

antibodies to alpha interferon neutralizing in vitro antiviral activity of alpha interferon

NN-Ab:

antibodies to alpha interferon non neutralizing in vitro antiviral activity of alpha interferon

NN-Ab-IFNβ :

antibodies to beta interferon non neutralizing in vitro antiviral activity of beta interferon

Pab:

polyclonal antibody

Phe:

phenylalanine

poly I:C:

polyinosinic:cytidylic acid

Pro:

proline

rIFNα:

recombinant alpha interferon

SDS-PAGE:

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Ser:

serine

Thr:

threonine

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Authors and Affiliations

  1. Dept. of Immunology and Infectious Diseases, Diagnostic Centre SSDZ, Delft, The Netherlands

    Paul Herbrink

  2. Institute for Inflammation Research, RHIMA Center, Rigshospitalet, Copenhagen, Denmark

    Morten Svenson

Authors
  1. Morten Svenson
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  2. Paul Herbrink
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Editor information

Editors and Affiliations

  1. Diagnostic Centre SSDZ, Delft, The Netherlands

    Huub Schellekens

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© 1997 Springer Science+Business Media Dordrecht

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Svenson, M., Herbrink, P. (1997). Measurement of cytokine antibodies. Test development. In: Schellekens, H. (eds) Antibodies in Cytokines. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5664-6_9

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  • DOI: https://doi.org/10.1007/978-94-011-5664-6_9

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6388-3

  • Online ISBN: 978-94-011-5664-6

  • eBook Packages: Springer Book Archive

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