Abstract
The beet cyst nematode (BCN, Heterodera schachtii Schm.) is a major pest of sugarbeet (Beta vulgaris L.). Chemical control of the parasite poses a threat to the environment and a wide crop rotation is economically impractical. Therefore, breeding of nematode resistant sugarbeet varieties is a promising alternative. Monogenic resistance has been introduced to cultivated sugarbeet from nematode resistant wild beet of the section Procumbentes by interspecific crosses. Different nematode resistant cytogenetic mutants have been selected from the offspring. However, the agronomic performance of these lines is poor and the transmission of the resistance reduced due to meiotic disturbances. Molecular markers have been developed suitable for marker-assisted selection (MAS) of nematode resistant plants in the breeding process and for the cloning of the BCN resistance gene. The novel positional cloning strategy described here makes use of repetitive wild beet specific DNA elements that are tightly linked to the resistance gene. The next step of the cloning procedure comprises the construction of representative yeast artificial chromosome (YAC) libraries and the subsequent screening with molecular markers to extract specific YAC clones encompassing the resistance locus. Candidate genes have been selected from root specific cDNA libraries using the YACs as probes. To identify the resistance gene a root specific complementation system has been established using Agrobacterium rhizogenes mediated transformation. Finally, transgenic hairy roots can be analysed for the presence of the BCN resistance gene by in vitro nematode testing.
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Kleine, M. et al. (1997). Breeding for Nematode Resistance in Sugarbeet: A Molecular Approach. In: Fenoll, C., Grundler, F.M.W., Ohl, S.A. (eds) Cellular and Molecular Aspects of Plant-Nematode Interactions. Developments in Plant Pathology, vol 10. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5596-0_14
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DOI: https://doi.org/10.1007/978-94-011-5596-0_14
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