Abstract
Heterologous expression of proteins can be dramatically improved as a result of optimized mRNA translation. 5’-Untranslated mRNA sequences (leaders of translation) are one of the possible targets for such an optimization. “Translational enhancers” for foreign protein expression in plants could be derived from 5’-leaders of genomic RNAs of positive-sense RNA viruses. Genomic RNA of these viruses functions as messenger RNA in infected plant cell and apparently efficiently competes with host mRNAs for the components of translation machinery. Alternatively, subgenomic RNAs for viral coat proteins, featuring their own translation initiation sites should be efficiently translated, since coat proteins are synthesized in a substantial amount during viral reproduction.
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Bagyan, I.L., Kraev, A.S., Pozmogova, G.E., Skryabin, K.G. (1997). The 5’-Untranslated Leaders of BSMV RNAgamma and PVX Coat Protein mRNA as Translational Enhancers in Tobacco Protoplasts. In: Nicolini, C. (eds) Genome Structure and Function. NATO ASI Series, vol 31. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5550-2_15
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DOI: https://doi.org/10.1007/978-94-011-5550-2_15
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