Abstract
Heterologous expression of proteins can be dramatically improved as a result of optimized mRNA translation. 5’-Untranslated mRNA sequences (leaders of translation) are one of the possible targets for such an optimization. “Translational enhancers” for foreign protein expression in plants could be derived from 5’-leaders of genomic RNAs of positive-sense RNA viruses. Genomic RNA of these viruses functions as messenger RNA in infected plant cell and apparently efficiently competes with host mRNAs for the components of translation machinery. Alternatively, subgenomic RNAs for viral coat proteins, featuring their own translation initiation sites should be efficiently translated, since coat proteins are synthesized in a substantial amount during viral reproduction.
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References
Current Protocols in Molecular Biology, unit 9.7A, John Willey and Sons, Inc., 1995.
Carrington, J.C., Freed, D.D. (1990) Cap-independent translation by a plant potyvirus 5’-nontranslated regionJ. Virol. 64, 1590–1597.
Gallie, D.R., Sleat, D.E., Watts, J.W., Turner, P.C., Wilson, T.M.A. (1987) The 5’-leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcriptsin vitroandin vivo,Nucl. Acids Res. 15, 3257–3273.
Gallie, D.R., Sleat, D.E., Watts, J.W., Turner, P.C., Wilson, T.M.A. (1987) A comparison of eukaryotic viral 5’-leader sequences as enhancers of mRNA expressionin vivo Nucl. Acids Res. 15, 8693–8711.
Jefferson, R.A., Kavanagh, T.A., Bevan, M.W. (1987) GUS fusions: bglucuronidase as a sensitive and versatile gene fusion marker in higher plantsEMBO J. 6, 3901–3907.
Jobling, S.A., Gehrke, L. (1987) Enhanced translation of chimaeric messenger RNA containing a plant viral untranslated leader sequenceNature325, 622–625.
Kozak, M. (1987) Effects of intercistronic length on the efficiency of reinitiation by eucaryotic ribosomesMol. Cell. Biol. 7, 3438–3445.
Kozlov, Iu.V., Afanas’ev, B.N., Rupasov, W., Golova, Iu.B., Kulaeva, O.I., Dolia, W., Atabekov, I.G., Baev, A.A. (1989) Primary structure of RNA3 of barley stripe mosaic virus and its variabilityMol. Biol. 23, 1080–1090.
Morozov, S.Yu., Miroshnichenko, N.A., Solovyev, A.G., Fedorkin, O.N., Zelenina, D.A., Lukasheva, L.I., Karasev, A.V., Dolja, V.V., Atabekov, J.G. (1991) Expression strategy of the potato virus X triple gene blockJ. Gen. Virology72, 2039–2042.
Nicolaisen, M., Johansen, E., Poulsen, G.B., Borkhardt, B. (1992) The 5’untranslated region from pea seedbome mosaic potyvirus RNA as a translational enhancer in pea and tobacco protoplastsFEBS Lett. 303, 169–172.
Petty, I.T.D., Edwards, M.C., Jackson, A.O. (1990) Systemic movement of an RNA plant virus determined by a point substitution in a 5’-leader sequenceProc.NatlAcad.Sci USA87, 8894–8897.
Pooggin, M.M., Skryabin, K.G. (1992) The 5’-untranslated leader sequence of potato virus X RNA enhancers the expression of a heterologous genein vivo Mol. Gen. Genet. 234, 329–331.
Skryabin, K.G., Kraev, A.S., Morozov, S.Yu., Rozanov, M.N., Chernov, B.K., Lukasheva, L.I., Atabekov, J.G. (1988) The nucleotide sequence of potato virus X RNANucl. Acids Res. 16, 10929–10930.
Sleat, D.E., Gallie, D.R., Jefferson, R.A., Bevan, M.W., Turner, P.V., Wilson, T.M.A. (1987) Characterization of 5’-leader sequence of tobacco mosaic virus RNA as a general enhancer of translationin vitro Gene 217, 217–225.
Turner, R., Bate, N., Twell, D., Foster, G.D. (1994)In vivocharacterization of a translational enhancer upstream from the coat protein open reading frame of potato virus S,Arch. Virol. 137,123–132.
Zelenina, D.A., Kulaeva, O.I., Smirnyagina, E.V., Solovyev, A.G., Miroshnichenko, N.A., Fedorkin, O.N., Rodionova, N.P., Morozov, S.Yu., Atabekov, J.G. (1992) Translation enhancing properties of the 5’-leader of potato virus X genomic RNAFEBS Lett. 296, 267–270.
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Bagyan, I.L., Kraev, A.S., Pozmogova, G.E., Skryabin, K.G. (1997). The 5’-Untranslated Leaders of BSMV RNAgamma and PVX Coat Protein mRNA as Translational Enhancers in Tobacco Protoplasts. In: Nicolini, C. (eds) Genome Structure and Function. NATO ASI Series, vol 31. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5550-2_15
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DOI: https://doi.org/10.1007/978-94-011-5550-2_15
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