Abstract
We review progress in the isolation and characterization of a avirulence genes from Pseudomonas syringae pv. pisi, which are responsible for the pattern of racecuitivar interactions with pea (Pisum sativum). The avirulence gene, avrPpiA1.R2 was obtained from the race 2 isolate 203 and was found to be chromosomally located in all race 2 strains examined, whereas homologues in races 5 and 7 were on plasmids. The gene, avrPpiB1.R3 was obtained from the race 3 isolate 870A and together with homologues from races 1 and 7 was shown to be plasmid-borne. Both genes have been sequenced and their products shown to share many features of other P. syringae avr genes. The recent isolation of a cosmid clone apparently harbouring the race 5 gene, avrPpiD, has confirmed the existence of the fifth avirulence gene postulated in the gene-for-gene scheme.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bavage, A.D., Vivian, A., Atherton, G.T., Taylor, J.D., and Malik, A.N. 1991. Molecular genetics of Pseudomonas syringae pv. pisi: plasmtd involvement in cultivar-specific incompatibiiity. J. Gen. Microbioi. 137: 2231–2239.
Bevan, J.R., Taylor, J.D., Crute, I.R., Hunter, P.J., and Vivian, A. 1995. Genetics of specific resistance in pea (Pisum sativum) cultivars to seven races of Pseudomonas syringae pv. pisi. Plant Pathol, 44: 98–108.
Cournoyer, B., Sharp, J.D., Astuto, A., Gibbon, M.J., Taylor, J.D., and Vivian, A. 1995 Moi. Plant-Microbe interact. 8: 700–708.
Dangt, J.D., Ritter, C., Gibbon, M.J., Mur, L.A.J., Wood, J.R., Goss, S., Mansfield, J., Taylor, J.D., and Vivian, A. 1992. Functional homologs of the Arabldopsis RPM1 disease resistance gene in bean and pea. Plant Cell 4: 1359–1369.
FillIingham, A.J., Wood, J., Bevan, J.R., Crute, I.R., Mansfield, J.W., Taylor, J.D., and Vivian, A. 1992. Avirulence genes from Pseudomonas syringae pathovars phaseolicola and pisi confer specificity towards both host and non-host species. Physioi. Mol. Plant Pathol. 40: 1–15.
Gibbon, M.J. 1994. Molecular characterization of an aviruience gene from race 2 of Pseudomonas syringae pv. pisi. Ph.D. thesis, UWE-Bristol, UK.
Innes, R.W., Bent, A.F., Kunkel, B.N., Sisgrove, S.R. and Staskawicz, B.J. 1993, Molecular analysis of avirufence gene avrRpt2 and identification of a putative regulatory sequence common to all known Pseudomonas syringae avirulence genes. J. Bacteriol. 175: 4859–4869.
Malik, A.N., Vivian, A., and Tayior, J.D. 1987. Isolation and partial characterization of three classes of mutant in Pseudomonas syringae pathovar pisi with altered behaviour towards their host, Pisum sativum. J. Gen. Microbiol. 133: 2393–2399.
Mansfield J., Jenner, C., Hockenhull, R., Bennett, M.A. and Stewart, R. (1994) Characterization of avrPphE, a gene for culttvar-specific aviruience from Pseudomonas syringae pv. phaseolicola which is physically linked to hrpY, a new hrp gene identified in the halo-blight bacterium. Mol. Plant-Microbe Interact. 7: 726–739.
Ritter, C. & Dangl, J.L. 1995. Trie avrRpm1 gene of Pseudomonas syringae pv. maculicola is required for virulence on Arabidopsis. Mol. Piant-Microbe Interact. 8: 444–453.
Staskawicz, B.J., Dahlbeck, D. and Keen, N. 1984. Cloned avirulence gene of Pseudomonas syringae pv. glycinea determines race-specific incompatibility on Glycine max (L.) Merr. Proc. Nati. Acad. Sci. USA 81: 6024–6028.
Vivian, A., and Mansfield, J. 1993. A proposal for a uniform genetic nomenclature for avirulence genes in phytopathogenic Pseudomonads. Mol. Plant-Microbe Interact. 6: 9–10.
Vivian, A., Atherton, G.T., Bevan, J.R., Crute, I.R., Mur, L.A.J., and Taylor, J.D. 1989. Isolation and characterization of cloned DNA conferring specific avirulence in Pseudomonas syringae pv. pisi to pea (Pisum sativum) cultivars, which possess the resistance allele, R2. Physiol. Mol. Plant Pathol. 34: 335–344.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Vivian, A., Cournoyer, B., Gibbon, M., Taylor, J. (1997). The Molecular Basis of Specificity in the Interaction Between Pseudomonas Syringae pv. pisi and Cultivars of Pisum sativum.. In: Rudolph, K., Burr, T.J., Mansfield, J.W., Stead, D., Vivian, A., von Kietzell, J. (eds) Pseudomonas Syringae Pathovars and Related Pathogens. Developments in Plant Pathology, vol 9. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5472-7_74
Download citation
DOI: https://doi.org/10.1007/978-94-011-5472-7_74
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6301-2
Online ISBN: 978-94-011-5472-7
eBook Packages: Springer Book Archive