Abstract
CGTase positive alkalophilic microorganisms were selected by parallel application of two screening techniques based on colorimetric distinction of the colonies at the cultivation plate level. One of the techniques uses Congo red and xylene cyanole dyes which form a clear halo only in the presence of γ-cyclodextrin (γ-CD), whereas the other uses phenolphthalein that is specific for β-cyclodextrin (β-CD). The CGTase produced by strains selected up to now yields, however, mainly β-CD. The production of β-CD by the CGTase of strain 37 is, nevertheless, very interesting since β-cyclodextrin concentration reached 16 mM at 50°C, pH 8.0, 10% w/v initial substrate concentration.
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© 1996 Springer Science+Business Media Dordrecht
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Matioli, G., Zanin, G.M., Guimarães, M.F., De Moraes, F.F. (1996). Screening For Gamma-Cgtase. In: Szejtli, J., Szente, L. (eds) Proceedings of the Eighth International Symposium on Cyclodextrins. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5448-2_11
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DOI: https://doi.org/10.1007/978-94-011-5448-2_11
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