Detection of antinuclear antibodies by immunofluorescence

  • R. L. Humbel

Abstract

The indirect immunofluorescence assay (IFA) is the most commonly used technique for the detection of antinuclear antibodies (ANA)[1]. It is a sensitive, reproducible and simple assay that can be performed in almost every laboratory. Because IFA enables the detection of a wide range of autoantibodies, it is generally used as a primary screening test for ANA[2].

Key words

Immunofluorescence autoantibodies autoantigens antinuclear antibodies 

Abbreviations

ANA

antinuclear antibodies

CIE

counter-immunoelectrophoresis

hnRNP

heterogeneous nuclear RNP

IFA

indirect immunofluorescence assay

JCA

juvenile chronic arthritis

RA

rheumatoid arthritis

RNP

ribonucleoprotein

SLE

systemic lupus erythematosus

snRNP

small nuclear RNP

SS

Sjögren’s syndrome

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Copyright information

© Springer Science+Business Media Dordrecht 1993

Authors and Affiliations

  • R. L. Humbel
    • 1
  1. 1.Centre Hospitalier de LuxembourgLuxembourg

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