Detection of antinuclear antibodies by immunofluorescence

  • R. L. Humbel


The indirect immunofluorescence assay (IFA) is the most commonly used technique for the detection of antinuclear antibodies (ANA)[1]. It is a sensitive, reproducible and simple assay that can be performed in almost every laboratory. Because IFA enables the detection of a wide range of autoantibodies, it is generally used as a primary screening test for ANA[2].

Key words

Immunofluorescence autoantibodies autoantigens antinuclear antibodies 



antinuclear antibodies




heterogeneous nuclear RNP


indirect immunofluorescence assay


juvenile chronic arthritis


rheumatoid arthritis




systemic lupus erythematosus


small nuclear RNP


Sjögren’s syndrome


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Copyright information

© Springer Science+Business Media Dordrecht 1993

Authors and Affiliations

  • R. L. Humbel
    • 1
  1. 1.Centre Hospitalier de LuxembourgLuxembourg

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