Abstract
A design of a flexible industrial scale bioreactor, for perfusion of mammalian cells, must meet process specific requirements and general design criteria for a production plant. A new spinfilter design has been developed for perfusion via an external loop of a bioreactor. The filter is operating in a glass cylinder with stainless steel top and bottom plates. Clogging by cell particles and proteins is minimized by the formation of taylor vortices at the surface of the 10 μm filter. During a 1400 hour perfusion experiment with hybridoma cells, various operating parameters were investigated with respect to retention of viable and dead cells. The rotational speed of the spinfilter, the recirculation rate of the cultivation fluid, and the dilution rate were varied. Optimum operating conditions were found under which the retention of viable cells is significantly higher than that of dead cells.
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References
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© 1997 Springer Science+Business Media Dordrecht
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Fraune, E., Meichsner, S., Kamal, M.N. (1997). A New Spinfilter Design. In: Carrondo, M.J.T., Griffiths, B., Moreira, J.L.P. (eds) Animal Cell Technology. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5404-8_44
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DOI: https://doi.org/10.1007/978-94-011-5404-8_44
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6273-2
Online ISBN: 978-94-011-5404-8
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