Abstract
Calcium has been studied for some time in animal cells because of its action as an intracellular second messenger. In recent years a similarly exciting role for calcium has been emerging in plant cells. To begin to understand the role of calcium in cellular processes it is essential to be able to measure fluctuations in the concentration of this ion, and for this reason developments in the technology of calcium measurement have increased along with the developing interest in calcium as a second messenger. Not all techniques that are useful for measuring cytosolic calcium in animal cells are suitable for use with plants. The use of calcium-sensitive fluorescent dyes has met with a number of problems in plants and fungi [1, 2]. Recombinant aequorin technology described in this chapter was developed in order to counter some of these problems. It also has advantages of its own [3].
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Knight, H., Trewavas, A.J., Knight, M.R. (1997). Recombinant aequorin methods for measurement of intracellular calcium in plants. In: Gelvin, S.B., Schilperoort, R.A. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5400-0_1
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DOI: https://doi.org/10.1007/978-94-011-5400-0_1
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