Abstract
It has been found that 200 micron-diameter spots of covalently immobilized analyte-specific capture reagent can significantly deplete analyte from several hundred microliters of liquid sample. The quantitative ligand binding assays which result sense the total analyte mass in a sample, rather than concentration, and provide high signal to background by concentrating analytes at the solid-liquid interface. Data from an avidin-biotin model shows the detection of 106 analyte molecules per spot. Preliminary results from a simultaneous multianalyte assay of the four human IgG subclasses validate the avidin-biotin results, and indicate sensitivity comparable to conventional ELISA methods requiring 100 times the antibody mass.
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References
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© 1998 Springer Science+Business Media Dordrecht
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Silzel, J.W., Dodson, C., Obremski, R.J., Tsay, T. (1998). Mass-Sensing Multianalyte Micro-Array Based Immunoassay by Direct Nir Fluorescence and Quantitative Image Analysis. In: Harrison, D.J., van den Berg, A. (eds) Micro Total Analysis Systems ’98. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5286-0_15
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DOI: https://doi.org/10.1007/978-94-011-5286-0_15
Publisher Name: Springer, Dordrecht
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