Laboratory on Chip for Clinical Applications

Abstract

For fast and continuous measurements of metabolic parameters small and easy-to-handle analytic devices with integrated chemo- and biosensors implemented in a fluidic microsystem were developed. Such microsystem was chosen for minimal invasive measurement, to overcome analyte handling problems and to perform continuous measurements.

Integrated sensors for measuring oxygen, CO₂, glucose, lactate, glutamine, glutamate concentrations and enzyme activities were produced using microelectronic technology and electrochemical detection principles. Different enzymes were immobilized in photopatterned pHEMA layers and used as biosensor array. A microfluidic allows continuous steady state measurements as well as performing flow protocols for the determination of enzyme activities.