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Preparation of extended DNA fibres for high resolution mapping by fluorescence in situ hybridization (FISH)

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Plant Molecular Biology Manual

Abstract

One of the objectives in genome research is the integration of genetic, molecular and cytogenetic maps. A major step towards the realization of this challenging goal involves the localization of the physical positions of DNA sequences within an entire set of chromosomes by fluorescence in situ hybridization (FISH). This technique permits the identification of entire genomes, individual chromosomes, subchromosomal regions and single copy genes and is, therefore, a highly versatile tool in studying a variety of genomic issues, e.g. interspecific hybrids, chromosome rearrangements, distribution of repetitive DNA sequences and gene mapping.

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Author information

Authors and Affiliations

  1. Department of Molecular Biology, Wageningen Agricultural University, Dreijenlaan 3, Wageningen, The Netherlands

    Paul Fransz & Pim Zabel

  2. Department of Genetics, Wageningen Agricultural University, Dreijenlaan 2, Wageningen, The Netherlands

    Hans de Jong

Authors
  1. Paul Fransz
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  2. Hans de Jong
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  3. Pim Zabel
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Editor information

Editors and Affiliations

  1. Purdue University, West Lafayette, Indiana, USA

    Stanton B. Gelvin

  2. Leiden University, Leiden, The Netherlands

    Robbert A. Schilperoort

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© 1998 Springer Science+Business Media Dordrecht

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Fransz, P., de Jong, H., Zabel, P. (1998). Preparation of extended DNA fibres for high resolution mapping by fluorescence in situ hybridization (FISH). In: Gelvin, S.B., Schilperoort, R.A. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5242-6_4

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  • DOI: https://doi.org/10.1007/978-94-011-5242-6_4

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-011-7657-6

  • Online ISBN: 978-94-011-5242-6

  • eBook Packages: Springer Book Archive

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