Abstract
In Phaseolus vulgaris (bean), PGIP is encoded by a gene family. We have isolated cDNA clones corresponding to two different pgip genes (pgip-1 and pgip-2). These genes have been separately expressed in Nicotiana benthamiana using the potato virus X (PVX) as a vector. PGIP-1 and PGIP-2 in crude protein extracts of PVX-infected TV. benthamiana, and a PGIP-1 purified from transgenic tomato plants carrying the pgip-1 coding sequence under the control of the CaMV 35S promoter, have been assayed for specificity of interaction with several fungal PGs. PGIP-1 from both plant sources exhibited a similar specificity, which was different from that of PGIP-2 and of the bulk PGIP purified from bean. Notably, PGIP-1 was unable to interact with homogeneous PG from Fusarium moniliforme, as determined by surface plasmon resonance analysis, while PGIP-2 and the bulk bean PGIP interacted with this enzyme.
Upon expression of a pgip-1-beta-glucuronidase (GUS) gene fusion in Nicotiana tabacum, GUS activity was detected mainly in the stigma and, to a lesser extent, in the anthers and in the conductive vascular tissue. The promoter responded to wounding but not to oligogalacturonides, fungal glucan, salicylic acid, cryptogein or pathogen infection. This expression pattern does not mirror that of the whole pgip gene family. Using specific sense and antisense primers which differentially amplify pgip-1 and pgip-2, RT-PCR experiments have been performed to individually analyze the expression of these genes in response to several treatments and stimuli. Indeed, pgip-1 transcripts accumulated in response to wounding, but not to elicitors, salicylic acid or infection, while pgip-2 transcripts accumulated in response to the stimuli examined. These observations indicate that pgip members have a different specificity and may be differentially regulated in P. vulgaris.
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Cervone, F. et al. (1998). Molecular Analysis of the Polygalacturonase-Inhibiting Protein (PGIP) Gene Family in Phaseolus Vulgaris L.. In: Kohmoto, K., Yoder, O.C. (eds) Molecular Genetics of Host-Specific Toxins in Plant Disease. Developments in Plant Pathology, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5218-1_34
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DOI: https://doi.org/10.1007/978-94-011-5218-1_34
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