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Isolation and Mode of Action of Ptr Chlorosis Toxin From Pyrenophora Tritici-Repentis

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Book cover Molecular Genetics of Host-Specific Toxins in Plant Disease

Part of the book series: Developments in Plant Pathology ((DIPP,volume 13))

Abstract

Pyrenophora tritici-repentis (Ptr) induces differentially tan necrosis and extensive chlorosis in its hexaploid wheat host. The necrosis symptom was shown to be associated with the Ptr necrosis toxin, a 14 kd protein [3], This toxin has been previously characterized by several independent laboratories and its encoding gene recently cloned [1,2]. A chlorosis inducing host-specific toxin, termed the Ptr chlorosis toxin, was isolated by our group from race 5 of Ptr. This toxin was found to be a hydrophilic protein, stable to exposure to organics [4] The objectives of the present investigations were to (1) develop an effective method to purify Ptr chlorosis toxin, and to (2) elucidate its mode of action.

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References

  1. Bailance, G.M., Lamari L., Kowatsch R. and Bemier C.C. (1996) ‘Cloning, expression and occurrence of the gene encoding the Ptr necrosis toxin from Pyrenophora tritici-repentis.’ Mol. Plant Pathology http://www.bspp.org.uk/mppol/1996/1209ballance

  2. Ciuffetti, L.M., Tuori R.P. and Gaventa J.M., (1997) ‘A single gene encodes a selective toxin causal to the development of tan spot of wheat’, Plant Cell 9, 135–144.

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  3. Lamari, L. and Bernier C.C. (1989) ‘Toxin of Pyrenophora tritici-repentis: host-specificity, significance in disease and inheritance of host-reaction’, Phytopathology 79, 740–744.

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  4. Orolaza, N.P., Lamari L. and Ballance G.M. (1995) ‘Evidence of a host specific toxin from Pyrenophora tritici-repentis, the causal agent of tan spot of wheat’, Phytopathology 85, 1282.

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© 1998 Springer Science+Business Media Dordrecht

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Strelkov, S., Lamari, L., Ballance, G.M., Orolaza, N.P. (1998). Isolation and Mode of Action of Ptr Chlorosis Toxin From Pyrenophora Tritici-Repentis . In: Kohmoto, K., Yoder, O.C. (eds) Molecular Genetics of Host-Specific Toxins in Plant Disease. Developments in Plant Pathology, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5218-1_15

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  • DOI: https://doi.org/10.1007/978-94-011-5218-1_15

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6197-1

  • Online ISBN: 978-94-011-5218-1

  • eBook Packages: Springer Book Archive

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