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Near-Infrared Dyes for High Technology Applications pp 183–190Cite as

NIR Fluorophores in Practical Analytical Chemistry

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Part of the book series: NATO ASI Series ((ASHT,volume 52))

Abstract

Fluorescence spectroscopy is a widely used spectroscopic tool in clinical and analytical chemistry applications. The near-infrared (NIR) region (650-1100 nm) of spectrum offers many advantages over the conventional UV-visible region, especially when background interference is a primary concern. We describe the development of practical applications using NIR fluorophores for immunodiagnostic assays. The procedure for covalent coupling of antibodies(Ab) to NIR fluorophores was systemically optimized in terms of pH, time, temperature and molar ratio. The optimum conditions for producing Ab with highest specific activity is described. We also describe the development of NIR fluorescence for detection of metal ions like aluminium and beryllium.

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Author information

Authors and Affiliations

  1. Department of Chemistry, Georgia State University, Atlanta, GA, 30303, USA

    Anand R. Swamy, Abraham George, Leila A. Tarazi, Gabor Patonay & Lucjan Strekowski

Authors
  1. Anand R. Swamy
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  2. Abraham George
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  3. Leila A. Tarazi
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  4. Gabor Patonay
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  5. Lucjan Strekowski
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Editor information

Editors and Affiliations

  1. Federal Institute for Materials Research and Testing, Berlin, Germany

    Siegfried Daehne  & Ute Resch-Genger  & 

  2. Institute of Analytical Chemistry, Chemo-and Biosensors, University of Regensburg, Regensburg, Germany

    Otto S. Wolfbeis

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© 1998 Springer Science+Business Media Dordrecht

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Swamy, A.R., George, A., Tarazi, L.A., Patonay, G., Strekowski, L. (1998). NIR Fluorophores in Practical Analytical Chemistry. In: Daehne, S., Resch-Genger, U., Wolfbeis, O.S. (eds) Near-Infrared Dyes for High Technology Applications. NATO ASI Series, vol 52. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5102-3_9

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  • DOI: https://doi.org/10.1007/978-94-011-5102-3_9

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6143-8

  • Online ISBN: 978-94-011-5102-3

  • eBook Packages: Springer Book Archive

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