Abstract
Extensive studies about the role of cytokinins on the development of higher plants has been published. Due to its pleiotropic action, the understanding of the physiological function of cytokinins is very difficult. To study the interaction between the cytokinins and the plastids, the response at the level of gene expression and photosynthetic activity was usually analysed by applying exogenous cytokinins. Using such experimental systems in which high, non-physiological concentrations of natural or synthetic cytokinins were applied at leaf discs, wound response and pharmacological effects can not be avoided. Moreover, two tools that have been valuable in determining the physiological functions of some plant hormones, namely, well-defined biosynthetic or perception mutants and chemical inhibitors of hormone action, are not available for cytokinins as stated by Kende and Zeevaart [1]. Therefore, in this work, a different model is being used. Transgenic tobacco plants in which the ipt-gene was introduced using the Agrobacterium tumefaciens transformation system, are studied. The ipt-gene, coding for the isopentenyl transferase, a key enzyme in the biosynthesis of cytokinin, is under the control of different promotors. Physiological effects of this gene were already extensively described by Beinsberger et al. [2], [3] and Estruch et al. [4].
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Kende, H. and Zeevaart, J.A.D. (1997), The five “classical” plant hormones, The Plant Cell 9, 1197–1210.
Beinsberger, S., Valcke, R., Deblaere, R., Clijsters, H., De Greef, J. and Van Onckelen, H. (1991) Effects of the introduction of Agrobacterium tumefaciens T-DNA ipt gene in Nicotiana tabacum L.cv. Petit Havana SR1 plant cells, Plant Cell Physiol. 32, 489–496.
Beinsberger, S., Clijsters, H., Valcke, R. and Van Onckelen, H. (1992) Morphological characteristics and phytohormone content of ipt-transgenic tobacco, in C.M. Karssen, L.C. van Loon and D. Vreugdenhill (eds), Progress in Plant Growth Regulation, Kluwer Acaddemic Publishers, Dordrecht, pp. 738–745.
Estruch, J., Prinsen, E., Van Onckelen, H., Schell, J. and Spena, A. (1991) Viviparous leaves produced by somatic activation of an inactive cytokinin-synthesizing gene, Science 254, 1364–1367.
Walker, D. (1990) The use of oxygen electrode and fluorescence probes in simple measurements of photosynthesis, Oxygraphics Ltd., Sheffield, UK.
van Kooten, O. and Snel, J. (1990) The use of chlorophyll nomenclature in plant stress physiology, Photosynthesis Research 25, 147–150.
Beinsberger, S., Valcke, R., Clijsters, H., De Greef, J. and Van Onckelen, H. (1992) Effects of enhanced cytokinin levels in ipt transgenic tobacco, in M. Kaminek, D.W.S. Mok and E. Zaž ímalová (eds.), Physiology and Biochemistryof cytokinins in Plants, SPB Acad. Publ. bv., The Hague, pp. 77–82.
Van Loven, K., Beinsberger, S., Valcke, R., Van Onckelen, H. and Clijsters, H. (1993) Morphometric analysis of the growth of Phsp-ipt transgenic tobacco plants, J. Exp. Botany 44(268), 1671–1678.
Schreiber, U., Neubauer, C. and Klughammer, C. (1989) Devices and methods for room-temperature fluorescence analysis, Phil. Trans. Roy. Soc. Lond. B323, 241–251.
Lichtenthaler, H. K. and Wellburn, A.R. (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents, Biochem. Soc. Trans. 11, 591–592.
Walker, D.A.and Osmond, C.B. (1986) Measurement of photosynthesis in vivo with a leaf disc electrode: correlations between light dependence of steady-state photosynthetic O2 evolution and chlorophyll a fluorescence transients, Proc. Roy. Soc. Lond. B227, 267–280.
Terashima, I. And Takenaka, A. (1986) Organisation of photosynthetic systems of dorsiventral leaves as adapted to the irradiation from the adaxial side, in R. Marcelle, Clijsters, H. and Van Poucke, M. (eds.), Biological Control of Photosynthesis, Martinus Nijhoff Publ., Dordrecht, pp. 219–299.
Synková, H., Van Loven, K., Pospíšilová, J. and Valcke, R. (1998) Photosynthesis of transgenic Pssu-ipt tobacco, submitted.
Synková, H., Van Loven, K. and Valcke, R. (1997) Increased content of endogenous cytokinins does not delay degradation of photosynthetic apparatus in tobacco, Photosynthetica 33(3–4), 595–608.
Baldewijns, L. and Valcke, R. (1998) Photosystem II electron transport in transgenic tobacco, Proc. Xith Intern. Congress on Photosynthesis, Budapest, XXX.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Valcke, R., Quanten, L. (1999). Structure and Function of the Photosynthetic Apparatus in Transgenic Tobacco. In: Argyroudi-Akoyunoglou, J.H., Senger, H. (eds) The Chloroplast: From Molecular Biology to Biotechnology. NATO Science Series, vol 64. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4788-0_53
Download citation
DOI: https://doi.org/10.1007/978-94-011-4788-0_53
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-5577-9
Online ISBN: 978-94-011-4788-0
eBook Packages: Springer Book Archive