Abstract
The aim of the investigations was to clarify whether cells adapted to the normal background radiation show enhanced resistance to the damaging effects of ionizing radiation. The underground laboratory for dosimetry and spectrometry (UDO) of the Physikalisch-Technische Bundesanstalt (PTB) at a depth of 925 m allowed to grow cells at a dose rate of 2.2 nSv/h compared with about 110 nSv/h of the normal background radiation. The yeast mutant rad54-3 which is temperature conditional for the repair of DNA double-strand breaks (dsb) was cultivated at 2.2 and 110 nSv/h, respectively, and irradiated in UDO using electrons (90Sr/90Y). This mutant rad54-3 allows to determine separately induction and repair of dsb, solely by cultivating irradiated cells at 36 °C (no dsb repair) or at 23 °C (with dsb repair). The results show that the normal background radiation does not adapt yeast cells for reduced initial dsb and elevated repair of dsb.
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References
Olivieri, G., Bodycote, J., Wolff, S. (1984) Adaptive response of human lymphocytes to low concentrations of radioactive thymidine. Science 223, 594–597.
Shadley, J.D., Wolff S. (1987) Very low doses of x-rays can cause human lymphocytes to become less susceptible to ionizing radiation. Mutagenesis 2, 95–96.
Cai, L., Liu, S.-Z. (1990) Induction of cytogenetic adaptive response of somatic and germ cells in vivo and in vitro by low doses of x-irradiation. Int. J. Radiation Biology 58, 187–194.
Shadley, J.D., Wiencke, J.-K. (1989) Induction of the adaptive response by x-rays is dependent on radiation intensity.Int. J. Radiation Biology 56, 107–118.
Shadley, J.D., Afzal, V., Wolff S. (1987) Characterization of the adaptive response to ionizing radiation induced by low doses of x-rays to human lymphocytes. Radiation Research 111, 511–517.
Wolff S., Wiencke, J.-K., Afzal, V., Joungblom, J., Cods, F. (1989) The adaptive response of human lymphocytes to very low doses of ionizing radiation: A case of induced chromosomal repair with the induction of specific proteins. In K.F. Baverstock and J.W. Stather (eds.) Low Dose-Radiation: Biological Bases of Risk Assessments. Taylor and Francis, London, pp. 446–454.
Wieneke, J.K., Afzal, V., Olivieri, G., Wol S. (1986) Evidence that the [3H] thymidine-induced adaptive response of human lymphocytes to subsequent doses of x-rays involves the induction of a chromosomal repair mechanism. Mutagenesis 1, 375–380.
Kelsey, K.T., Memisoglu, A., Frenkel, D., Liber, H.L. (1991) Human lymphocytes exposed to low doses of x-rays are less susceptible to radiation-induced mutagenesis. Mutation Research 263, 197–201.
Shadley, J.D., Dai, G. (1992) Cytogenetic and survival responses in G1 phase human lymphocytes. Mutation Research 265, 273–281.
Azzam, E.I., de Toledo, S.M., Raaphorst, G.P., Mitchel, R.E.J. (1992) Radiation-induced radioresistance in normal human skin fibroblast cell line. In T. Sugahara, L.A. Sagan, T. Aoyama (eds.) Low Dose Irradiation and Biological Defense Mechanisms, Elsevier Science Publishers, Amsterdam, pp. 291–294.
Azzam, E.I., Raaphorst, G.P., Mitchel, R.E.J. (1994) Radiation-induced adaptive response for protection against micronucleus formation and neoplastic transformation in C3H 10T1/2 mouse embryo cells, Radiation Research Suppl. 138, S28–S31.
Zhou, P.-K., Sun, W.-Z., Liu, X.-Y, Zhang, Y.-P., Wei, K. (1992) Adaptive response of mutagenesis and DNA double-strand break repair in mouse cells induced by low doses of y-rays. In T. Sugahara, L.A. Sagan, T. Aoyama (eds.) Low Dose Irradiation and Biological Defense Mechanisms, Elsevier Science Publishers, Amsterdam, pp. 271–274.
Koch, C.J., Giandomenico, A.R. (1994) Do low doses of radiation induce a response modulating induction or repair of DNA single-strand breaks? Radiation Research Suppl. 138, S89–S92.
Boreham, D.R. and Mitchel, R.E.J. (1991) DNA lesions that signal the induction of radioresistance and DNA repair in yeast, Radiation Research 128, 19–28.
Satta, L., Augusti-Tocco, G., Cundan, E., Esposito, A., Fiore, M., Paggi, P., Poggesi, F., Ricordy, R., Scarsella, G. (1992) Biological response of the yeast Sacch. cerev. to chemical radiomimetic agents: Influence of low background environment. In: see lit. 10. pp. 383–386.
Peßara, W., Lauterbach, U. (1993) Ein extrem strahlungsarmes Untergrundlaboratorium fir Dosimetrie und Spektrometrie (UDO). Tagung des Fachverbandes far Strahlenschutz in Binz auf Rügen, FS-93–67-T, 455–460.
Budd, M., Mortimer, R.K. (1982) Repair of double-strand breaks in a temperature conditional radiation-sensitive mutant of Sacch. cer. Mutation Research 33, 157–164.
Frankenberg, D., Frankenberg-Schwager, M., Harbich, R. (1984) Split-dose recovery is due to the repair of DNA double-strand breaks. Int. J. Radiation Biology 46, 541–553.
Böhm, J., Billion, Ph., Simoen, J.-P. (1976) Intercompanson of the PTB and LMRI standards in beta dosimetry. PTB-Dos-1.
Frankenberg-Schwager, M., Frankenberg, D., Harbich, R. (1987) Potentially lethal damage repair is due to the difference of DNA double-strand break repair under immediate and delayed plating conditions. Radiation Research 111, 192–200.
Frankenberg-Schwager, M., Frankenberg, D., Bloecher, D., Adamczyk, C. (1979) The influence of oxygen on the survival and yield of DNA double-strand breaks in irradiated yeast cells. Int. J. Radiation Biology 36, 261–270.
Ward, J.F. (1994) DNA damage as the cause of ionizing radiation-induced gene activation. Radiation Research Supplement 138, S85–S88.
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Frankenberg, D., Helmstaedter, K., Boehm, J., Pessara, W., Frankenberg-Schwager, M. (1999). Environmental Radiation Does Not Enhance the Tolerance of Yeast Cells to Acute Radiation Exposure. In: Baumstark-Khan, C., Kozubek, S., Horneck, G. (eds) Fundamentals for the Assessment of Risks from Environmental Radiation. NATO Science Series, vol 55. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4585-5_17
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DOI: https://doi.org/10.1007/978-94-011-4585-5_17
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