Using Various Biomarkers for the Estimation of the Situation after an Accident at Siberian Chemical Plant

  • N. N. Ilyinskikh
  • A. T. Natarajan
  • I. I. Suskov
  • S. N. Kolyubaeva
  • I. I. Danilenko
  • L. N. Smirennii
  • A. Yu. Yurkin
  • E. N. Ilyinskikh
Part of the NATO Science Series book series (ASEN2, volume 54)


On April 6, 1993 near the town of Tomsk (Russia) there was an accident at the Siberian chemical plant (SCP) as a result of which an area of 200 square kilometres was polluted with radionuclides. Especially dangerous was the fallout of the plutonium-239; there were also traces of caesium-137 and cobalt-60. Scientists from Russia, Ukraine and The Netherlands have participated in the investigations of radiation doses. A cytogenetic method and investigation of tooth enamel by the method of electronic spin resonance (ESR), as well as micronuclei test were used to estimate the radioactive doses received by the population. The ESR signal intensity and cytogenetic aberration frequency in lymphocytes of the tooth donors showed a good correlation. The data obtained testify that 15% of the inhabitants of the Samus settlement received a dose of radiation effect exceeding 100 centrigray (cGy). In 87% of these cases there was reasonable agreement between laboratories. The distinctions concerned the results of the examinations of the fishermen where the method of ESR gave high results (80-210 cGy) and chromosomic method and micronuclei gave low ones (8-52 cGy). A large number of cytogenetically aberrated cells were especially observed in the people born between 1961 and 1969. It was found that during these years, serious failures at the Siberian chemical combine occurred causing radiation pollution of the district. The number of cells with cytogenetic aberrations was considerably less in the people arriving in Samus after 1980. Our experience shows the importance of collecting detailed information on the donors from whom samples were taken for radiation dose reconstruction.


Siberian Chemical Plant radionuclides micronucleus test tooth enamel 


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  1. 1.
    Ilyinskikh, N.N. (1995) Radiating ecogenetics of Tomsk area. Tomsk, Siberian Med. Univ., 80pp.Google Scholar
  2. 2.
    Richvanov, L.P. (1994) Environmental state and population health in Siberia Chemical plant zone. State ecological committee of Tomsk region. p.84.Google Scholar
  3. 3.
    Beninson, D., Lloid, D.C. and Natarajan, A.T. (1986) Biological dosimetry: chromosomal aberration analysis for dose assessment. IAEA, Vienna (Technic. Reports Ser. No. 260).Google Scholar
  4. 4.
    Edwards, A.A., Lloid, D.C., Prosser, J.S. (1989) Chromosome aberrations in human lymphocytes - A radiobiological review. In: Low Dose Radiat.: Biol. Bases Risk Assessment. London, 423–432.Google Scholar
  5. 5.
    Lloid, D.C., Edwards, A.A. (1983) Chromosome aberrations in human lymphocytes: effect of radiation quality, dose and dose rate. In: Radiation-induced chromosome damage in man. New York: Alan R. Liss Inc., 23–49.Google Scholar
  6. 6.
    Lloid, D.C., Edward, A.A., Leonard, A. (1988) Frequencies of chromosomal aberrations induced in human blood lymphocytes by low doses of X-rays. Int. J. Radiat. Biol. 53 (1), 49–55.CrossRefGoogle Scholar
  7. 7.
    Piatkin, E.K., Filiushkin, I.V. and Nugia, V. Ju. (1986) The evaluation of evenity of irradiation by man peripheral blood lymphocytes cytogenetic investigation. Therapevtichesky archiv (Russia) 58 30–33.Google Scholar
  8. 8.
    Shevchenko, V.A., Snigiryova, G.P., Suskov, I.I., Akayeva, E.A., Elisova, T.N., Iofa, E.L., Nivola, I.N., Kostina, L.N., Novitskaya, N.N., Sidorova, V.F., Nazins, E.D. (1995) The cytogenetic effects among the Altai region population exposed to ionising radiation resulting from Semipalatinsk nuclear tests. Radiation biology. Radioecology 35 (5), 588–596.Google Scholar
  9. 9.
    Almassy, Z., Krepinsky, A.B., Bianco, A. and Kutles, G.J. (1987) The present state and perspectives of micronucleus assay in radiation protection. A review. Appl. Radiai. Isol. 38 (4), 241–249.CrossRefGoogle Scholar
  10. 10.
    Fenech, M., Morley, A.A., (1986) Cytokinesis-block micronucleus method in human lymphocytes: effect of in vivo ageing and low dose X-irradiation. Mut. Res. 161 193–198.CrossRefGoogle Scholar
  11. 11.
    Kolubaeva, S.N., Miasnikova, L.V., Raketskaja, V.V., Komar, V.V. (1991) Using of micronucleus test for indication of postradioactive effects in man. Recommendations of the Central Scientific Research Institute of Radiology. St Petersburg, p.14.Google Scholar
  12. 12.
    Littlefield, J.G., Sayer, A.M., Frome, E.J. (1989) Comparison of dise-response parameters for radiation-induced acentric fragments and micronuclei observed in cytokinesis-arrested lymphocytes. Mutagenesis 4 (4), 265–270.CrossRefGoogle Scholar
  13. 13.
    Prosser, J.S., Mognet, J.E., Lloid, D.C., Edwards, A.A. (1989) Radiation induction of micronuclei in human lymphocytes. Mut. Res. 199 (1), 37–45.Google Scholar
  14. 14.
    Ikeya, M., Miyajima, J., Okajiama, S. (1984) ESR dosimetry for atomic bomb survivors using shell buttons and tooth enamel. Jap. J. Appl. Rhys. 23 (9), 687–699.Google Scholar
  15. 15.
    Nakamura, N., Iwasaki, M., Miyazawa, C., Niwa, K., Akiyama, M., Sawada, S., Awa, A.A. (1996) Radiation dose assessment by electron spin resonance measurement on tooth enamel from atomic-bomb survivors. Radiation and Risk 7 253–258.Google Scholar
  16. 16.
    Pass, B., Aldrich, J.E. (1985) Dental enamel as in vivo radiation dosimeter. Med. Phys. 12 (3), 305–307.CrossRefGoogle Scholar
  17. 17.
    Skvortzov, V.G., Ivannikov, A.I., Eichhoff, U. (1995) Assessment of individual accumulated irradiation doses using EPR-spectroscopy of tooth enamel. J. Molec. Struc. 347 321–329.CrossRefGoogle Scholar
  18. 18.
    Buckton, K.E., Evans, H.J. (1973) (eds.) Methods for the analysis of human chromosome aberrations. WHO, Geneva.Google Scholar
  19. 19.
    Sevankaev, A.V. (1991) The contemporary condition of quantitative evaluation of cytogenetic effects in the range of minor radiation doses. Radiology 31 (4), 600–605.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1999

Authors and Affiliations

  • N. N. Ilyinskikh
    • 1
  • A. T. Natarajan
    • 2
  • I. I. Suskov
    • 3
  • S. N. Kolyubaeva
    • 4
  • I. I. Danilenko
    • 5
  • L. N. Smirennii
    • 6
  • A. Yu. Yurkin
    • 1
  • E. N. Ilyinskikh
    • 1
  1. 1.Siberian Med. Univ.TomskRussia
  2. 2.Dept. Radial. Genet. & Chem. Mutag., Leiden UnivLeidenNetherlands
  3. 3.lnstitute Gen. GenetMoscowRussia
  4. 4.lnstitute RadiologicSt PetersburgRussia
  5. 5.lnst. EpidemilogicKievUkraine
  6. 6.Research Centre of Spacecraft Radiation SafetyMoscowRussia

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