Abstract
In all biological photoreceptors (energy converters and signal sensors) light excitation generates sequences of several transients of lifetimes in the pico- to milliseconds range before eventually signal transduction takes place. The signal event is thus determined by the protein conformational changes concomitant with and subsequent to the primary event in the chromophore (e.g., isomerization or electron transfer). Due to the overlapping absorption spectra of the transients and the parent system in most photoreceptors, the study of the transient properties (such as yield and decay lifetimes) by optical methods is difficult if not impossible. Time-resolved evolution of the lighttriggered temperature and pression changes in solution, as studied by photothermal and photobaric methods (e.g., laser-induced optoacoustic spectroscopy, LIOAS), yields information on the lifetimes of these intermediates as well as on the enthalpic and structural changes (related to entropic changes) taking place upon formation of the intermediates, without recoursing to their optical properties [1, 2].
Keywords
- Protein Conformational Change
- Protonated Schiff Base
- Sensory Rhodopsin
- Halobacterium Halobium
- Photothermal Method
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Braslavsky, S.E. and Heibel, G.E., Chem. Rev. 92 (1992) 1381.
Schulenberg, P. and Braslavsky, S.E. In Progress Phototherm. Photoacoustic. Sc. Technol. III: Life and Earth Sciences, Mandelis, A., Hess, P. (Eds) SPIE Press, 1997, pp. 58–81.
Crippa, P.R, Vecli, A., Viappiani, C. J., Photochem. Photobiol. B: Biol. 24 (1994) 3.
Schulenberg, P.J., Gärtner, W., Braslavsky, S. E., J. Phys. Chem. 99 (1995) 9617.
Gensch, T., Hellingwerf, K.J., Braslavsky, S.E., and Schaffner, K., J. Phys. Chem. A, 102 (1998) 5398.
Gensch, T., Strassburger, J.M., Gärtner, W., and Braslavsky, S.E., Isr. J. Chem. (1999) in press.
Losi, A., Braslavsky, S., Gärtner, W., Spudich, J., Biophys. J. (1999) in press.
Churio, M.S., Angermund, K.P., and Braslavsky, S.E., J. Phys. Chem. 98 (1994) 1776.
Borsarelli, CD., Braslavsky, S.E., J. Phys. Chem. B 102 (1998) 6231.
Wegewijs, B., Verhoeven, J., Braslavsky, S.E., J. Phys. Chem. 100 (1996) 8890.
Wegewijs, B., Paddon-Row, M.N., Braslavsky, S.E., J. Phys. Chem. A 102 (1998) 8812.
Borsarelli, C.D., Braslavsky, S.E., J. Phys. Chem. B 103 (1999) in press.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Braslavsky, S.E. (1999). Biological photosensors studied by laser-induced optoacoustic spectroscopy. In: Greve, J., Puppels, G.J., Otto, C. (eds) Spectroscopy of Biological Molecules: New Directions. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4479-7_44
Download citation
DOI: https://doi.org/10.1007/978-94-011-4479-7_44
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5919-0
Online ISBN: 978-94-011-4479-7
eBook Packages: Springer Book Archive