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Protein-Protein Interactions in Ethylene Signal Transduction in Arabidopsis

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Biology and Biotechnology of the Plant Hormone Ethylene II

Abstract

We are using molecular genetic approaches in Arabidopsis thaliana in order to elucidate the mechanisms of ethylene signal transduction. In particular, we are interested in understanding how the ETR1 ethylene receptor and its homologs signal to the downstream protein kinase, CTR1. A starting point for addressing this question is to examine protein-protein interactions involving the receptors and CTRl. To detect protein interactions, we are utilizing the yeast two-hybrid assay as well as in vitro protein association assays. Using these methods, we are analyzing the association between the putative regulatory domain of CTR1 and the predicted cytoplasmic portions of both ETR1 and ERS1. In addition, we have been screening yeast two-hybrid libraries in order to identify proteins that interact with the CTR1 amino-terminal domain and/or the ETR1, ETR2 and ERS1 ethylene receptors.

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© 1999 Springer Science+Business Media Dordrecht

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Chang, C. et al. (1999). Protein-Protein Interactions in Ethylene Signal Transduction in Arabidopsis. In: Kanellis, A.K., Chang, C., Klee, H., Bleecker, A.B., Pech, J.C., Grierson, D. (eds) Biology and Biotechnology of the Plant Hormone Ethylene II. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4453-7_13

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  • DOI: https://doi.org/10.1007/978-94-011-4453-7_13

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5910-7

  • Online ISBN: 978-94-011-4453-7

  • eBook Packages: Springer Book Archive

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