Abstract
The concept of transposable elements (TEs) as purely selfish elements is being challenged as we have begun to appreciate the extent to which TEs contribute to allelic diversity, genome building, etc. Despite these long-term evolutionary contributions, there are few examples of TEs that make a direct, positive contribution to adaptive fitness. In E. coli cryptic (silent) catabolic operons can be activated by small TEs called insertion sequences (IS elements). Not only do IS elements make a direct contribution to fitness by activating cryptic operons, they do so in a regulated manner, transposing at a higher rate in starving cells than in growing cells. In at least one case, IS elements activate an operon during starvation only if the substrate for that operon is present in the environment. It appears that E. coli has managed to take advantage of IS elements for its own benefit.
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References
Blattner, F.R., G. Plunket III, C.A. Bloch, N.T. Pema, V. Burland, M. Riley, J. Collado-Vides, J.D. Glasner, C.K. Rode, G.F. May-hew, J. Gregor, N.W. Davis, H.A. Kiekpatrick, M.A. Goeden, D.J. Rose, B. Mau & Y. Shao, 1997. The complete genome sequence of Escherichia coli K-12. Science 277: 1453–1462.
Defez, R. & M. deFelice, 1981. Cryptic operon for β-glucoside metabolism in Escherichia coli K12: genetic evidence for a regulatory protein. Genetics 97: 11–25.
DiNardo, S., K.A. Voelkel, R. Sternglanz, A.E. Reynolds & A. Wright, 1982. Escherichia coli DNA topoisomerase I mutants have compensatory mutations in DNA gyrase genes. Cell 31: 43–51.
Doolittle, W.F. & C. Sapienza, 1980. Selfish genes, the phenotype paradigm and genome evolution. Nature 284: 601–603.
Giel, M., M. Desnoyer & J. Lopilato, 1996. A mutation in a new gene, bglJ, activates the bgl operon in Escherichia coli K-12. Genetics 143:627–635.
Hall, B.G., 1978. Regulation of newly evolved enzymes. IV Directed evolution of the ebg repressor. Genetics 90: 673–691.
Hall, B.G., 1981. Changes in the substrate specificities of an enzyme during directed evolution of new functions. Biochemistry 20: 4042–4049.
Hall, B.G., 1983. Evolution of new metabolic functions in the laboratory. pp. 234–257 in Evolution of Genes and Proteins, edited by M. Nei and R. Koehn, Sinauer Associates, Sunderland, Mass.
Hall, B.G., 1995. Evolutionary potential of the ebgA gene. Mol. Biol. Evol. 12:514–517.
Hall, B.G., 1998. Activation of the bgl operon by adaptive mutation. Mol. Biol. Evol. 15: 1–5.
Hall, B.G., 1999. The spectra of spontaneous growth-dependent and adaptive mutations in ebgR. J. Bacteriol. 181:1149–1155.
Hall, B.G. & P.W. Betts, 1987. Cryptic genes for cellobiose utilization in natural isolates of Escherichia coli. Genetics 115: 431–439.
Hall, B.G., P.W. Betts & J.C. Wootton, 1989. DNA sequence analysis of artificially evolved ebg enzyme and ebg repressor genes. Genetics 123: 635–648.
Hall, B.G. & D.L. Hartl, 1974. Regulation of newly evolved enzymes. I. Selection of a novel lactase regulated by lactose in Escherichia coli. Genetics 76: 391–400.
Hall, B.G. & D.L. Hartl, 1975. Regulation of newly evolved enzymes. II. The ebg repressor. Genetics 81: 427–435.
Hall, B.G. & L. Xu, 1992. Nucleotide sequence, function, activation, and evolution of the cryptic asc operon of Escherichia coli K12. Mol. Biol. Evol. 9: 688–702.
Hall, B.G., S. Yokoyama & D. Calhoun, 1983. Role of cryptic genes in microbial evolution. Mol. Biol & Evol. 1: 109–124.
Jurka, J. & V.V. Kapitonov, 1999. Sectorial mutagenesis by transposable elements. Genetica 107: 239–248.
Kricker, M. & B.G. Hall, 1984. Directed evolution of cellobiose utilization in Escherichia coli. Mol. Biol. & Evol. 1: 171–182.
Kricker, M. & B.G. Hall, 1987. Biochemical genetics of the cryptic gene system for cellobiose utilization in Escherichia coli K12. Genetics 115:419–429.
Li, W.-H., 1984. Retention of cryptic genes in microbial populations. Mol. Biol. Evol. 1: 212–218.
Mahadeven, S., A. E. Reynolds & A. Wright, 1987. Positive and negative regulation of the bgl operon of Escherichia coli. J. Baceriol. 169: 2570–2578.
Orgel, L.E. & F. H. Crick, 1980. Selfish DNA: the ultimate parasite. Nature 284: 604–607.
Pardue, M.-L. & P.G. DeBaryshe, 1999. Drosophila telomeres: two transposable elements with important roles in chromosomes. Genetica 107: 189–196.
Parker, L.L. & B. G. Hall, 1988. A fourth E. coli gene system with the potential to evolve β-glucoside utilization. Genetics 119: 485–490.
Parker, L.L. & B.G. Hall, 1990a. Characterization and nucleotide sequence of the cryptic cel operon of E. coli K12. Genetics 124: 455–471.
Parker, L.L. & B.G. Hall, 1990b. Mechanisms of activation of the cryptic cel operon of E. coli K12. Genetics 124: 473–482.
Prasad, I. & S. Schaefler, 1974. Regulation of the β-glucoside system in Escherichia coli K12. J. Bacteriol. 120: 638–650.
Reizer, J., A. Reizer & M.H. Saier, Jr, 1990. The cellobiose permease of Escherichia coli consists of three proteins and is homologous to the lactose permease of Staphylococcus aureus. Res.Microbiol. 141: 1061–1067.
Schnetz, K., 1995. Silencing of Escherichia coli bgl promoter by flanking sequence elements. EMBO J. 14: 2545–2550.
Schnetz, K. & B. Rak, 1988. Regulation of the bgl operon of Escherichia coli by transcriptional anti-termination. EMBO J. 7: 3271–3277.
Schnetz, K. & B. Rak, 1992 IS5: a mobile enhancer of transcription in Escherichia coli. Proc. Natl. Acad. Sci. USA 89: 1244–1248.
Schnetz, K., C. Toloczyki & B. Rak, 1987. β-glucoside (Bgl) operon of Escherichia coli K12: nucleotide sequence, genetic organization, and possible evolutionary relationship to regulatory components of two Bacillus subtilis genes. J. Bacteriol. 169: 2579–2590.
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Hall, B.G. (2000). Transposable elements as activators of cryptic genes in E. coli . In: McDonald, J.F. (eds) Transposable Elements and Genome Evolution. Georgia Genetics Review 1, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4156-7_20
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DOI: https://doi.org/10.1007/978-94-011-4156-7_20
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