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Floral Homeotic Genes for Genetic Engineering of Reproductive Sterility in Poplars

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Book cover Somatic Cell Genetics and Molecular Genetics of Trees

Part of the book series: Forestry Sciences ((FOSC,volume 49))

Abstract

Many floral homeotic genes are expressed early in floral development and exhibit floral-specific expression, making them excellent candidates for use in engineering reproductively sterile plants. We have cloned and partially characterized Populus trichocarpa cDNAs and genes homologous to the Arabidopsis/Antirrhinum genes LEAFY (LFY)/ FLORICAULA (FLO), APETALA3 (AP3)/DEFICIENS (DEF), and AGAMOUS (AG)I PLENA (PLE). The P. trichocarpa LFY homolog, PtFL, shares 75% amino acid identity with LFY. PtD shows 81% amino acid similarity with DEF, and PtAG shows 85% homology withAG. PtFL is expressed in immature inflorescences on which floral primordia are developing. PtD is expressed strongly in stamen primordia from the onset of organogenesis, and is expressed at low levels in carpel primordia.

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References

  1. Strauss, S.H., Rottmann, W.H., Brunner, A.M., Sheppard, L.A.: Genetic engineering of reproductive sterility in forest trees, Molecular Breeding 1 (1995), 5–26.

    Article  CAS  Google Scholar 

  2. Rottmann, W.H., Sheppard, L.S., Brunner. A.M., Strauss, S.H. (manuscript in preparation)

    Google Scholar 

  3. Ma, H.: The unfolding drama of flower development: recent results from genetic and molecular analyses, Genes & Development 8(1994), 745–756.

    Article  CAS  Google Scholar 

  4. Pneuli, L., Haraven, D., Rounsley, S.D., Yanofsky, M.F., Lifschitz, E.: Isolation of the tomato AGAMOUS gene TAG1 and analysis of its homeotic role in transgenic plants, The Plant Cell 6 (1994), 163–173.

    Google Scholar 

  5. Kempin, S.A., Mandel, M. A., Yanofsky, M.F.: Conversion of perianth into reproductive organs by ectopic expression of the tobacco floral homeotic gene NAG1, Plant Physiol. 103 (1993), 1041–1046.

    Article  PubMed  CAS  Google Scholar 

  6. Tsuchirnoto, S., van der Krol, A.R., Chua, N-H.: Ectopic expression of pMADS3 in transgenic petunia phenocopies the petunia blind mutant, The Plant Cell 5 (1993) 843–853.

    Google Scholar 

  7. Lu, Z-H., Wu, M., Lon, C-S., Yeong, C-Y., Goh, C-J.: Nucleotide sequence of a flower-specific MADS box cDNA clone from orchid, Plant Mol. Bio. 23 (1993), 901–904.

    Article  CAS  Google Scholar 

  8. Schmidt, R. J., Velt, B., Mandel, M.A., Mena, M., Hake, S., Yanofsky, M.F.: Identification and molecular characterization of ZAG1, the maize homolog of the Arabidopsis homeotic gene AGAMOUS, The Plant Cell 5 (1993), 729–737.

    PubMed  CAS  Google Scholar 

  9. Tandre, K., Albert, V.A., Sundâs, A., Engström, P.: Conifer homologs to genes that control floral development in angiosperms, Plant Molecular Biology 27 (1995), 69–78.

    Article  PubMed  CAS  Google Scholar 

  10. Weigel, D., Alvarez, X, Smyth, D.R., Yanofsky, M.F., Meyeriwitz, E.M.: LEAFY controls floral meristem identity in Arabidopsis, Cell 69 (1992) 843–859.

    Article  PubMed  CAS  Google Scholar 

  11. Coen, E.S., Romero, J.M., Doyle, S., Elliot, R., Murphy, G., Carpenter, R.: FLORICAULA: ahomeotic gene required for flower development inAntirrhinum majus, Cell 63 (1990), 1311–1322.

    Article  PubMed  CAS  Google Scholar 

  12. Weigel, D., Nilsson, O.: A developmental switch sufficient for flower initiation in diverse plants, Nature 12 (1995), 495–500.

    Article  Google Scholar 

  13. Meyerowitz, E.M., Coen, E.S.: The war of the whorls: genetic interactions controlling flower development, Nature 353 (1991), 31–37.

    Article  PubMed  Google Scholar 

  14. Yanofsky, M.F., Ma, H., Bowman, J.L., Drews, G.N., Feldmann, K.A., Meyerowitz, E.M.: The protein encoded bythe Arabidopsis homeotic gene agamous resembles transcription factors, Nature 346 (1990), 35–39.

    Article  PubMed  CAS  Google Scholar 

  15. Davies, B., Schwarz-Sommer, Z.: Control of floral organ identity by homeotic MADS-box transcription factors, Results and Problems in Cell Differentiation 20, L. Nover (ed.), Plant Promoters and Transcription Factors, Springer-Verlag, Berlin (1994).

    Google Scholar 

  16. Schwarz-Sommer, Z., Huijser, P., Nacken, W., Saedler, H., Sommer, H.: Genetic control of flower development by homeotic genes inAntirrhinum majus, Science 250 (1990) 931-936.

    Google Scholar 

  17. Sambrook, J., Fritsch, E.F., Maniatis, T.: Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, N.Y., 1989.

    Google Scholar 

  18. Kelly, A.I, Bonnlander, M.B., Meeks-Wagner, D.R.: NFL, the tobacco homolog of FLORICAULA and LEAFY, istranscriptionally expressed in both vegetative and floral meristems, The Plant Cell 7 (1995), 225–234.

    PubMed  CAS  Google Scholar 

  19. Rottmann, W.H., Boes, T.K., and Strauss, S.H.: Structure and expression of a leafy homolog from Populus. J. Cell. Biochem. Suppl 17B 23 (1993).

    Google Scholar 

  20. Sheppard, L.S. Rottmann, W.H., Strauss, S.H.: Floral homeotic genes for engineering sterility in poplars: cDNA cloning of a Populus homolog of DEFICIENS, International Union of Forestry Research Organizations molecular genetics workshop, Prout’s Neck, Maine. May 20-23,1994.

    Google Scholar 

  21. Rottmann, W.H., Sheppard, L.S., Brunner. A.M., Strauss, S.H. (manuscript in preparation)

    Google Scholar 

  22. Sheppard, L.S., Rottmann, W.H., Brunner, A.M., Strauss, S.H. (manuscript in preparation)

    Google Scholar 

  23. Kelly, A. J. et al, ibid.

    Google Scholar 

  24. Schwarz-Sommer, Z., Hue, I., Huijser, P., Flor, P.J, Hansen, R., Tetens, F., Lonnig, W.-E., Saedler, H., and Sommer, H.: Characterization of theAntirrhinum floral homeotic MADS-box gene deficiens: evidence for DNA binding and autoregulation of its persistent expression throughout flower development. EMBO 11 (1992), 251–263.

    CAS  Google Scholar 

  25. Pnueli, L., Abu-Abeid, M., Zamir, D., Nacken, W., Schwarz-Sommer, Z., Lifschitz, E.: The MADS box gene family in tomato: temporal expression during floral development, conserved secondary structures and homology with homeotic genes fromAntirrhinum and Arabidopsis, The Plant Journal 1 (1991), 255–266.

    Article  PubMed  CAS  Google Scholar 

  26. Hardenack, A., Ye, D., Saudler, H., Grant, S.: Comparison of MADS box gene expression in developing male and female flowers of the dioecious plant white campion, The Plant Cell 6 (1994), 1775–1787.

    PubMed  CAS  Google Scholar 

  27. Schwarz-Sommer et al 1992. ibid.

    Google Scholar 

  28. Trobner, W., Ramirez, L., Motte, P., Hue, I, Huijser, P., Lonnig, W.-E., Saedler, H., Sommer, H., and Schwarz-Sommer, Z.: Globosa: a homeotic gene which interacts with deficiens in the control of Antirrhinum floral organogenesis. EMBO 11 (1992), 693–4704.

    Google Scholar 

  29. van der Krol, A.R., Brunelle, A, Tsuchimoto, S., Chua, N.: Functional analysis of petunia floral MADS box genepMADS1, Genes & Development 7 (1993), 1214–1228.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Forest Science, Oregon State University, Corvallis, Oregon, 97331-7501, USA

    L. A. Sheppard, A. M. Brunner, W. H. Rottmann, R. Meilan & S. H. Strauss

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  1. L. A. Sheppard
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  2. A. M. Brunner
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  3. W. H. Rottmann
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  4. R. Meilan
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  5. S. H. Strauss
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Editor information

Editors and Affiliations

  1. Federal Research Centre for Forestry and Forest Products, Institute of Forest Genetics, Grosshansdorf, Germany

    M. Raj Ahuja

  2. Laboratorium voor Genetica, Flanders Interuniversity Institute for Biotechnology, Gent, Belgium

    Wout Boerjan

  3. USDA Forest Service, Institute of Forest Genetics, Berkeley, CA, USA

    David B. Neale

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© 1996 Springer Science+Business Media Dordrecht

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Sheppard, L.A., Brunner, A.M., Rottmann, W.H., Meilan, R., Strauss, S.H. (1996). Floral Homeotic Genes for Genetic Engineering of Reproductive Sterility in Poplars. In: Ahuja, M.R., Boerjan, W., Neale, D.B. (eds) Somatic Cell Genetics and Molecular Genetics of Trees. Forestry Sciences, vol 49. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3983-0_23

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  • DOI: https://doi.org/10.1007/978-94-011-3983-0_23

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5761-5

  • Online ISBN: 978-94-011-3983-0

  • eBook Packages: Springer Book Archive

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