Molecular Evolution of C4-Form Pepc in Maize: Comparison of Primary Sequences and Kinetic Properties with a Newly Cloned Recombinant Root-Form PEPC

  • L.-Y. Dong
  • T. Masuda
  • S. Hata
  • K Izui
Chapter

Abstract

Phosphoenolpyruvate carboxylases (PEPC, EC4.1.1.31) are encoded by a small mutigene family in plants. Each isoform PEPC is thought to play a specific physiological role. Three isoforms have been reported in C4 plants such as maize [1, 2], sorghum [3] and Flaveria trinervia [4]; the C4-form PEPC involved in C4 photosynthesis, the C3-form PEPC for housekeeping and the root-form PEPC prevalent in roots. Only for sorghum full-length cDNAs for the three isoforms were cloned and sequenced [3]. Although we previously cloned and sequenced cDNAs for C4-form [5, 6] and C3-form PEPCs [7] in maize, the third one has remained unknown. We report here the cloning and prokaryotic expression of a full-length cDNA for the root-form PEPC and the comparison of its kinetic and regulatory properties with the C4-form PEPC [8]. Structural features of C4-form PEPCs acquired during evolution of C4 photosynthesis are discussed.

Key words

C metabolism enzymes isoforms cDNA cloning allosteric effectors phosphorylation/dephosphorylation 

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Copyright information

© Springer Science+Business Media Dordrecht 1998

Authors and Affiliations

  • L.-Y. Dong
    • 1
  • T. Masuda
    • 1
  • S. Hata
    • 1
  • K Izui
    • 1
  1. 1.Division of Applied Biosciences, Graduate School of AgricultureKyoto UniversitySakyo-ku, KyotoJapan

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