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Regulatory Phosphorylation of Maize C4-Form PEP Carboxylase (PEPC): Antibody Detection System and Purification of PEPC-protein Kinase

  • Y. Ueno
  • J. Emura
  • K. Y. Kumagaye
  • K. Nakajima
  • K. Inami
  • T. Shiba
  • S. Hata
  • K. Izui
Chapter

Abstract

Activity of phosphoeno/pyruvate carboxylase (PEPC) for C4 photosynthesis is upregulated by light (reviewed in [1, 2]). The regulation is mediated by a specific protein kinase (PEPC-PK) that phosphorylates Ser near N-terminus (Ser-15 in the maize Ca-form PEPC). This phosphorylation is dependent on light intensity in Ca-plants [3]. Hartwell e al [4] reported that PEPC-PK was regulated by the level of translatable mRNA. No evidence, however, has been obtained to indicate how PEPC-PKs or its mRNAs are regulated and there has been no report about the gene for PEPC-PK.

Key words

chemiluminescence circadian rhythm enzymes light regulation signal transduction synthetic oligopeptide 

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References

  1. 1.
    Chollet, R., Vidal, J. and O’Leary, M. H. (1996) Annu. Rev. Plant Physiol. Plant Mol. Biol. 47, 273–298CrossRefPubMedGoogle Scholar
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    Vidal, J. and Chollet, R. (1997) Trends in Plant Sei. 2, 230–237CrossRefGoogle Scholar
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    Bakrim, N., Echevarria, C., Cretin, C., Arno-Dupont, M., Pierre, J. N., Vidal, J., Chollet, R. and Gadal, P (1992) Eur. J. Biochem. 204, 821–830CrossRefPubMedGoogle Scholar
  4. 4.
    Hartwell, J., Smith, L. H., Wilkins, M. B., Jenkins, G. I. and Nimmo, H. G. (1996) Plant J. 10, 1071–1078CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1998

Authors and Affiliations

  • Y. Ueno
    • 1
  • J. Emura
    • 2
  • K. Y. Kumagaye
    • 2
  • K. Nakajima
    • 2
  • K. Inami
    • 2
  • T. Shiba
    • 2
  • S. Hata
    • 1
  • K. Izui
    • 1
  1. 1.Div. Applied Biosciences, Grad. Sch. AgricultureKyoto Univ.KyotoJapan
  2. 2.Peptide Institute, Inc.OsakaJapan

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